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Biomedical subjects

C F Simpson

Publications and source records attributed to C F Simpson.

At least 163 records · Page 9Linked to original sources
PubMed1965

The nature of bands in parasitized bovine erythrocytes.

Anaplasma marginale is the etiological agent of a hemolytic disease of cattle, known as anaplasmosis. The organism appears as a marginal inclusion in parasitized erythrocytes, but certain isolates also have bands associated with the inclusion. Inclusions and associated bands in parasitized erythrocytes in the liver and peripheral circulation were studied by light microscope cytochemistry and electron microscopy. Bands were comet- and dumbbell-shaped by light microscopy and were stained by techniques used to demonstrate protein and fibrin. The same forms, as well as other shapes, were seen in infected erythrocytes which were sectioned and examined by electron microscopy. Bands had longitudinal and transverse periodicity. They did not appear to have a crystalline structure. Their appearance was collated with that of bovine fibrin. Bands were well differentiated in erythrocytes that were entensively hemolyzed by natural or artificial means, but poorly differentiated in mildly hemolyzed erythrocytes. Hemolysis methods appeared to influence the morphology of bands and their demonstration.

Anaplasma↗
PubMedDate not supplied

Applications of capillary electrophoresis in nephrology.

Capillary electrophoresis has recently emerged as a powerful technique for separating components in biological samples. A family of separation methods capable of handling a diverse range of samples has been developed, the sample volumes required are very small and a wide range of specialised detectors can be employed. This review examines some methods with particular application to the analysis of urine and tubular fluid samples and references relevant applications.

Amino Acids↗
PubMedDate not supplied

Propranolol effects on hypertension and the arterial wall beyond the treatment period in turkeys.

To determine if the effects of propranolol on hypertension and the arterial wall persisted for a significant time after the medication was discontinued, various parameters were compared at 16 weeks in control turkeys (CC), in birds on the medication from 3 days to 16 weeks (PP), and in others that were treated only from 3 days to 10 weeks (PC). At 16 weeks, arterial blood pressure, maximum rate of pressure increase (dp/dt max), and aortic intimal hyperplasia were lowest in the PP group, intermediate in the PC birds, and highest in the CC turkeys. Likewise, the vascular wall constituted 61% of the radius of the coronary arteries in the CC group, but only 52% and 45% in the PC and PP groups, respectively. At 16 weeks, heart rate was lowest and aortic tensile strength highest in the group that was treated for the entire period, at the end of which the average plasma propranolol level was 97 ng/ml. At the same age, heart rate and aortic tensile strength were approximately the same in the CC and PC groups, and propranolol was not detectable in the plasma. It is concluded that the administration of propranolol to hypertensive turkeys early in life reduced blood pressure, aortic intimal hyperplasia, and arterial wall thickness and that these effects persisted to a significant degree for at least 6 weeks after the medication was discontinued. The higher aortic tensile strength that was produced by propranolol did not persist.

Animals↗
PubMedDate not supplied

Pacheco's parrot disease of psittacine birds.

Pacheco's parrot disease was identified as the cause of death of 3 psittacine birds at an aviary. Confirming a previous report, a herpesvirus was found to be the etiologic agent. The virus induced mortality in embryonated chicken eggs and budgerigars. The virus was characterized by electron microscopy, filtration experiments, and ether-sensitivity. Hepatic lesions in chick embryos and budgerigars were similar by light- and electron-microscope studies. Naked and enveloped nucleocapsids were seen in the nucleus and cytoplasm of infected hepatocytes, and naked virions were present in the sinusoids of the liver. Multinucleated cells in the liver contained virus in both intranuclear and intracytoplasmic inclusions; the latter inclusions were both membrane-bound and not. Vaccination of budgerigars with a commercial lyophilized Marek's disease vaccine did not prevent infection with the Pacheco's disease herpesvirus.

Animals↗