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Biomedical subjects

C Finlay

Publications and source records attributed to C Finlay.

9 recordsLinked to original sources
PubMed1992

[A comparison of 3 single-dose plans for mebendazole in the treatment of trichuriasis].

People infected by Trichuris trichiura were selected in a community by the Kato-Katz technique. The study included a universe of 376 persons, male and female, positive and asymptomatic, monoparasitic and multiparasitic. They were divided in treatment groups with not less than 50 people. Monoparasitic patients were treated with 500, 400 and 300 mg of mebendazole, and multiparasitic patients with 500 and 400 mg of mebendazole. In all the cases the drug was used in single doses and its administration was supervised by the physician. Therapeutical response in monoparasitic and multiparasitic patients is shown. It is suggested to use mebendazole single doses of 300 mg in monoparasitic and of 400 mg in multiparasitic patients with an intensiveness of less than 5,000 h/g, as an alternative for treating asymptomatic populations with T. trichiura.

Adolescent↗
PubMed1991

Group membership, strength of group identification and perceptions of violence for locations in Northern Ireland.

118 undergraduate students, all of Northern Irish origin, were asked to rate 60 locations in Northern Ireland for violence and denominational composition. As expected from Social Identity Theory, subjects perceived ingroup locations as less violent than outgroup ones. Contrary to expectations, however, there was no increased differentiation of violence judgements with increasing strength of identity, for which two possible explanations were considered.

Humans↗
PubMed1989

Mutation is required to activate the p53 gene for cooperation with the ras oncogene and transformation.

Previous experiments have brought into question which amino acid sequence of the p53 oncogene product should be considered wild type and whether the normal protein is capable of cooperating with the ras oncogene to transform cells in culture. To address these questions, a series of p53 cDNA-genomic hybrid clones have been compared for the ability to cooperate with the ras oncogene in transformation assays. From these experiments, it has become clear that the amino acid alanine at position 135, in either the genomic clone or the cDNA clone, failed to produce a p53 protein that cooperated with the ras oncogene and transformed cells. Replacing alanine with valine at this position in either the genomic or the cDNA clone activated for transformation in this assay. Using restriction enzyme polymorphisms in the p53 gene, it was shown that normal mouse DNA encodes alanine at position 135 in the p53 protein. Thus, mutation is required to activate the p53 protein for cooperation with the ras oncogene. After cotransfection with the activated ras gene, the genomic p53 DNA clone always produced more transformed cell foci (1.7-fold) than similar cDNA clones and these foci were more readily cloned (3.6-fold) into permanent cell lines. A series of deletion mutants of the genomic p53 clone were employed to show that the presence of intron 4 in the p53 gene was sufficient to provide much enhanced clonability of transformed foci from culture dishes. The presence of introns in the p53 gene constructions also resulted in elevated levels of p53 protein in the p53-plus-ras-transformed cell lines. Thus, qualitative changes in the p53 protein are required to activate p53 for transformation with the oncogene ras. Quantitative improvements of transformation frequencies are associated with the higher expression levels of altered p53 protein that are provided by having one of the p53 introns in the transforming plasmid.

Amino Acid Sequence↗
PubMed1987

Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice.

A line of transgenic mice containing the simian virus 40 (SV40) large tumor antigen gene under the control of the viral enhancer-promoter expressed this viral protein in the brains of these mice within the first 2 weeks after birth. Multiple foci of anaplastic cells formed in the choroid plexuses of these mice at 36 to 41 days after birth, and normal tissue coexisted with these transformed foci. Immunoperoxidase staining to detect the SV40 T antigen showed tumor-specific expression of nuclear T antigen at late times in tumor development, approximately 90 to 100 days and thereafter. The level of SV40 T antigen, on a per cell basis, appeared to be lower in the great majority of choroid plexus cells at earlier times in tumor development. These results suggest that low levels of tumor antigen (14 to 36 days) are present before detectable pathology (36 to 41 days) and the level of T antigen per cell is higher in rapidly growing late-stage tumors (older than 90 days).

Animals↗
PubMed1975

Changes in cellular enzyme levels and the inhibition of selective release of lysosomal hydrolases from macrophages by indomethacin.

Indomethacin increases the cellular levels of several lysosomal enzymes in cultures of mouse peritoneal macrophages exposed to the drug for periods of time ranging from one day to four weeks. This increase can be blocked by puromycin, an inhibitor of protein synthesis. Pretreatment of macrophages with indomethacin inhibits the selective release of lysosomal enzymes induced by a C-mucopolysaccharide peptidoglycan complex purified from the cell walls of Group A streptococci.

Animals↗