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C Fiol

Publications and source records attributed to C Fiol.

35 records · Page 2Linked to original sources
PubMed1994

[Effectiveness of hypercholesterolemia detection in high risk individuals compared to opportunistic detection].

BACKGROUND: The aims of this study were to estimate the number of people who should receive some intervention (pharmacologic and/or dietetic) to reduce cholesterol concentrations and to evaluate selective case finding in comparison to opportunistic detection. METHODS: Six hundred twenty-five individuals participating in a study of cardiovascular risk factors were included in the study. Those with total cholesterol concentrations (TC) greater than 6.2 mmol/l and those with CT concentrations greater than 5.2 mmol/l observed upon lipid profile analysis and history of cardiovascular disease and/or risk factors were considered as candidates. Out of these individuals, those with cholesterol concentrations linked to low density lipoproteins greater than 4.14 mmol/l or greater than 3.37 mmol/l and previous history of cardiovascular disease or two risk factors were considered candidates to undergo intervention to reduce cholesterol concentrations. Moreover, the number of individuals with CT concentrations greater than 6.2 mmol/l which would not be detected if CT was only determined in those who already had another cardiovascular risk factor was estimated. RESULTS: 37.5% (CI 95%; 33.8%-41.5%) of the individuals required lipid profile and out of these 88.8% (CI 95%; 3.8%-92.4%) were candidates to receive intervention. Upon evaluating the efficacy of the strategy of selective case finding it was observed that the sensitivities of the risk factors were low, ranging from 22.6% in those with family history of cardiovascular disease to 34.8% in cases with personal history of high blood pressure. CONCLUSIONS: If selective case finding of high risk subjects was the only strategy applied, many individuals with hypercholesterolemia would remain undetected, therefore the strategy of opportunistic detection is preferable whenever possible.

Adult↗
PubMed1994

An enzyme-linked immunosorbent assay method to measure human apolipoprotein E levels using commercially available reagents: effect of apolipoprotein E polymorphism on serum apolipoprotein E concentration.

A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for human apolipoprotein E (apo E) quantification using commercially available reagents is described. The assay is a noncompetitive, sandwich ELISA in which the wells were coated with a monoclonal EO1 antibody anti-human apo E and detected with a polyclonal antibody-peroxidase conjugate anti-apo E. The mean apo E concentration in 168 middle-aged subjects randomly selected from general population was 51.7 +/- 12.4 mg/liter. Apo E levels were highly correlated with apo E phenotypes. Apo E polymorphism, which shows a modulating effect in the catabolism of apo E containing lipoproteins, may explain a large fraction, 18.5%, of the variability of serum apo E levels in middle-aged population. Isoforms apo E2 and apo E4 have an opposite effect on the regulation of serum apo E concentrations. Individuals that express apo E2 isoform present higher apo E levels (65.5 mg/liter for apo E2/E3), whereas the average of individuals with apo E4 is lower (42.8 mg/liter for apo E4/E3) than general population.

Adult↗
PubMed1991

[Early detection of dyslipidemias. Is the isolated determination of total cholesterol efficient?].

BACKGROUND: The aim of this study was to analyze the percentage of individuals with hypoalphalipoproteinemia and isolated hypertriglyceridemia which would not be detected if only total cholesterol were included in the initial detection of dyslipemia. METHODS: Five hundred forty-one individuals participating in a study concerning factors of cardiovascular risk were included in the present study which consisted in a survey on risk factors and a medical examination. The population studied was divided according to the concentration of total cholesterol (TC) in desirable concentrations (5.2 mmol/l), intermediate (5.2-6.2 mmol/l) and elevated (6.2 mmol/l). The concentrations of cholesterol bound to high density lipoproteins (cHDL) less than 0.9 mmol/l and of triglycerides (TG) greater than 2.3 mmol/l were considered as high risk. RESULTS: Hypoalphalipoproteinemia would not be detected in 2.9% of the population studied (IC 95%: 1.5%-4.3%) and isolated triglyceridemia in 2.4% (IC 95%: 1.1%-3.7%) if the cHDL and the TG were only determined in the individuals who had high or elevated CT concentrations and two or more cardiovascular risk factors. CONCLUSIONS: These data support the efficacy of CT as the only test for initial detection of dyslipemia and question the convenience of initial quantification of cHDL and triglycerides in all cases as some authors request.

Adult↗
PubMed1990

[Factors associated with generalized forms of arteriosclerosis in patients with coronary disease].

We studied the lipidic metabolism and the atherogenic risk factors non related to lipidic metabolism in two groups of patients: group 1 with only coronary disease and group 2 with coronary disease and atherosclerosis in the cerebral and/or peripheric artery territories. Patients of group 2 showed a cholesterol, cLDL, and B-apoprotein titers significantly higher than group 1. Systolic and diastolic blood pressure were higher in group two. Also the incidence of diabetes mellitus in group 2 was higher than in group 1. In a stepwise discriminating analysis the diastolic arterial pressure and cholesterol titers were the parameters with a greatest predictive potential for the presence of localized or generalized ischemic disease. B-apoprotein was the lipidic parameter with the greatest predictive potential. All together these data suggest that in coronary patients, a discrete increase in plasma cholesterol can imply a risk of suffering ischemia in some other arterial territories. Diastolic blood pressure, cholesterol and diabetes mellitus are the factors with the highest predictive potential for the generalization of arteriosclerosis.

Arteriosclerosis↗
PubMed1980

[HDL cholesterol as a risk factor for arteriosclerosis (author's transl)].

Recent studies have demonstrated a negative correlation between the cholesterol content of the HDL fraction of serum lipoproteins and the incidence of arteriosclerosis. In this study the limits of normality of HDL-cholesterol have been determined in a male and female population, followed by an analysis of the HDL-cholesterol in patients with myocardial infarction. Normal values for total cholesterol in males and females were, respectively, 194.7 +/- 61.8 mg/dl and 188 +/- 62.8 mg/dl, and those of HDL-cholesterol, 63.1 +/- 14.6 mg/dl and 70.3 +/- 24.4 mg/dl. Total cholesterol in the patients with myocardial infarction was not significantly different from that of the control group, but HDL-cholesterol was significantly lower (p < 0.05).

Adult↗
PubMed1980

Calcium fluxes in isolated pure cholinergic nerve endings from the electric organ of Torpedo marmorata.

1. Ca fluxes were studied in a pure cholinergic preparation of nerve endings from Torpedo electric organ. In standard physiological saline solution (280 mM Na+, 3 mM K+) the calcium uptake was at the rate of 8 nmole per mg of protein per minute, equivalent to a calcium influx of 0.6 pmole/cm2 x sec. 2. The rate of calcium uptake was enhanced when synaptosomes were depolarized, increasing potassium concentration in the external medium. Maximum stimulation was reached when the potassium concentration was over 50 mM and averaged 20 nmole Ca2+ per mg protein per minute, equivalent to calcium influx of about 1.5 pmole/cm2 x sec. 3. Protoveratrine and tytiustoxin also stimulated calcium uptake into the nerve terminals and their effect was blocked by tetrodotoxin. Tetrodotoxin didn't block calcium uptake in K+-stimulated synaptosomes. Some inhibitors of transmitter release such as Dantrolene, Verapamil, BetaBungarotoxin and adenine nucleotides prevented the stimulatory effect on calcium uptake by depolarizing agents. 4. The calcium entry increased roughly linearly with a slope proportional to external calcium concentrations up to 20 mM. This effect was greatly increased when either 100 mM K+ or protoveratrine were added to the external medium. 5. Most of the loss of isotope from 45Ca-loaded synaptosomes occurred by a sodium-dependent calcium efflux mechanism with half-activation at 13 mM Na+. This may be equivalent to 1.65 pmole/cm2 x sec. 6. In sodium-free and calcium-free solutions, a residual efflux (5 nmole per mg protein per min) is observed. Metabolic inhibitors such as CN- enhanced this residual efflux. 7. Morphological studies using sodium-free fixation solutions and radioautographic detection of 45Ca are consistent with calcium sequestration by intracellular organelles by a similar mechanism to the calcium buffering system described in disrupted synaptosomes. Synaptic vesicles may play an important role in this calcium sequestration.

Adenine Nucleotides↗