C Firmin — Page 2 | PubMed Health

PubMed1978

[Bamifylline. Conditions of its interference in the estimation of urinary 17-oxosteroids. Method of estimation using Zimmermann's reaction (author's transl)].

Bamifylline in therapeutic dosage may markedly disturb the determination of urinary 17-oxosteroids by Zimmermann's reaction, although the conditions of extraction and formation of this chromogenic substance are not always optimal for the metabolites of bamifylline. It is easy to suspect this interference as the chromogenic substance is produced as soon as the reagents are introduced, contrary to 17-oxosteroids, and it presents a more bluish colour. It is possible to separate completely both types of metabolite by washing the chloroform extracts with acid solutions which form salts with bamifylline and thus extract it. From these acid solutions, bamifylline may be recovered and estimated Zimmermann's reaction which is, for this purpose, modified.

17-Ketosteroids↗

PubMedDate not supplied

[Long-term treatment of Cushing's disease using ketoconazole. Possibility of therapeutic escape].

Five women suffering from Cushing's disease were treated with ketoconazole 800 mg per day for 2 to 28 months (mean 12.4 months). Four of them had full clinical and biochemical regression. However, after 8 months of therapy the disease failed to respond in three of these four women. Increasing the ketoconazole dosage up to 1,200 mg per day was ineffective in two patients. Such an escape phenomenon, not described until now, will restrict the use of ketoconazole in the treatment of Cushing's disease, although the drug is easy to administer and well tolerated globally and by the liver in most cases.

Adrenocorticotropic Hormone↗