PubMed Health⌕ Search

Biomedical subjects

C Fournel

Publications and source records attributed to C Fournel.

23 records · Page 2Linked to original sources
PubMed1983

Identification of canine T lymphocytes by membrane receptor to peanut agglutinin: T-lymphocyte identification in dogs with lupus-like syndrome.

Canine T lymphocytes were detected, using fluorescent peanut agglutinin (PNA) as a marker. Using a fluorescent technique and cytofluorometry, 70 +/- 11% and 72.4%, respectively, of peripheral blood lymphocytes were bound to PNA. Of thymocytes, 97 +/- 4.5% were detected by fluorescent PNA, but less than 1% were detected for lymphocytes from bone marrow. The T-lymphocyte depletion and enrichment indicated that PNA was bound to lymphocytes recognized by anti-T-lymphocyte heterologous serum. A T-lymphocyte deficiency was detected among 8 dogs with a lupus-like syndrome.

Animals↗
PubMed1980

Clinical and laboratory features of canine lupus syndromes.

Determination of the specificities of antinuclear antibodies in the sera of 20 dogs presenting with symptoms of a lupus-like syndrome permitted their separation into 2 groups. The first group of 14 dogs all had antibody activity to DNA-histone antigen(s), and 4 of them also had antibodies to native DNA (nDNA). The Farr test with standard buffer was found to be unsatisfactory for the measurement of anti-nDNA antibodies in dog sera due to a high incidence of false positive reactions; these could be eliminated by the inclusion of sodium dodecyl sulfate in the buffer system. The second group of 6 dogs was characterized by the presence of antibodies to extractable nuclear antigen. In every serum tested diseased dogs had a diminished level of circulating thymic factor as compared to controls of the same age, suggesting that a diminution of suppressor T cells may be an etiologic factor.

Animals↗
PubMedDate not supplied

Contribution of the feline Langerhans cell to the FIV model.

In order to identify an equivalent of Langerhans cells in cat stratified epithelia, we used a panel of monoclonal antibodies known to be reactive with membrane antigens present on human Langerhans cells. The labelling was carried out by immunoperoxidase staining, for examination by light microscope, and by immunogold labelling, for electron microscopy. Out of 18 antibodies tested, only one, MHM23 antibody, specific against CD18 antigen, presented reactivity with dendritic epithelial cells on either frozen sections, epidermal sheets or cell suspension cytospins. On the ultrastructural level, these clear, dendritic, CD18+ cells showed "zipper-like" shapes similar to Birbeck granules, which are characteristic ultrastructural markers of Langerhans cells. This observation favours the hypothesis that these CD18+ cells in cat stratified epithelia are the equivalent of human Langerhans cells. These labelled cells were found in all epidermal locations and in the mucous membranes (oral, vaginal, rectal and oesophageal membranes). As feline immunodeficiency virus (FIV) transmission may occur through these membranes, the involvement of these feline Langerhans cells was studied in cats seropositive for FIV.

Animals↗
PubMedDate not supplied

Ultrasonographic appearance of intra-abdominal granuloma secondary to retained surgical sponge.

This report describes two animals (one dog and one cat) with a retained surgical sponge. Both had nonspecific clinical signs. Clinical examination, ultrasonography and cytologic examination were used to identify an abdominal mass compatible with a granuloma. The lesions were surgically removed and confirmed histologically as granulomas secondary to a retained sponge. The ultrasonographic appearance was very similar in both animals.

Abdomen↗