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Biomedical subjects

C G Culbertson

Publications and source records attributed to C G Culbertson.

18 recordsLinked to original sources
PubMed1992

Identification of Acanthamoeba in bronchoalveolar lavage specimens.

Acanthamoeba species infect humans occasionally and act as opportunistic pathogens in immunocompromised individuals. This study demonstrates the application of cytocentrifugation as an aid to identification of Acanthamoebae. In addition, certain staining procedures clearly optimized visualization of characteristic amoebic features. This was demonstrated by adding amoebae from laboratory cultures to bronchoalveolar lavage specimens. In preparations stained by the Papanicolaou, trichrome, or hematoxylin-eosin (H&E) procedures, the discrete deeper staining nucleolus was the most distinctive feature. The vacuolated cytoplasm also aided in the identification of amoebae. These features were less apparent and often distorted following staining of Acanthamoeba species with the Hema III and Giemsa procedures.

Acanthamoeba↗
PubMed1984

Pathogenic free-living amebae. Immunocytologic demonstration and species identification.

This report describes methods for preparation, immunologic marking, and staining of amebae in formalin-fixed brain tissue, and in exudates, discharges and body fluids. The suggested procedures provide slide preparations of unknown specimens together with known amebae from cultures as controls, all on a single slide for each antiserum. This allows for cytologic studies of host cells, morphology of the amebae present, and specific immune marking of the amebae by use of immune sera and protein A Staphylococcus. Such preparations may also be used for immunoperoxidase and immunofluorescence. The need for the utilization of direct microscopic examination by the methods suggested here, or comparable ones, is discussed.

Agglutination Tests↗
PubMed1981

Amebic meningoencephalitis.

As the result of research by many workers, information as to the accurate diagnosis and possibly effective treatment has accumulated. While much more definitive academic investigation will aid in improving the results of medical management, the next objective should be the serious study of patients with suggestive histories and with acute and chronic respiratory ailments for the possible role of free-living amebas therein. The state of the art has reached the point where the two free-living amebic infections can probably be diagnosed by direct examination of exudates from the respiratory tract before the diseases become serious and beyond treatment. At present, the lack of effort to do this is as if one were trying to diagnose amebic dysentery histolytica in the diagnosis of entamebiasis. There is need for increased effort by local health officials, parents, clinicians and laboratory scientists to attempt to prevent the tragic consequences of naegleramebiasis by all possible means, at facilities near the patient's home. While the prospects of effectively handling the problem of acanthamoebiasis are less promising, earlier recognition of it in patients now known likely to develop such an infection might lead to better understanding the approaches to its treatment.

Amebiasis↗
PubMed1980

Surface coagglutination with formalinized, stained protein A staphylococci in the immunologic study of three pathogenic amebae.

The indirect immunofluorescence and, to a lesser extent, the immunoperoxidase methods have become the principal means of conducting immunological studies upon amebae. These procedures require the conjugation of fluorescein or enzyme with antiglobulin reagents and fixation of the amebae. The present report describes a somewhat similar methodology wherein formalinized, stained protein A staphylococci combined with specific antibody have been found to coagglutinate upon a portion of the amebic surface of living trophozoites in a fashion similar to direct immunofluorescence. The prepared staphylococci without attached antibody have also been found to coagglutinate in an identical fashion after the specific antibody has been deposited upon the amebic surface. This is comparable to indirect immunofluorescence reaction. A comparison of this procedure with the immunofluorescence method of identification and with measurement of titer of serum antibody is presented, which indicates that the new technique compares favorably with the immunofluorescence results.

Agglutination Tests↗
PubMed1975

Soil ameba infection. Specific indirect immunoenzymatic (peroxidase) staining of formalin-fixed paraffin sections.

This report describes the use of the immunoenzymatic (peroxidase) method to identify the species and to stain distinctively the amebas in formalin-fixed paraffin-mounted sections. This permits the use of hematoxylin and eosin counterstaining. The method, now well developed by others for many purposes, is an alternative to immunofluorescence and seems to offer a number of advantages and a lesser number of disadvantages.

Amebiasis↗
PubMed1965

Oncogenicity of the simian adenoviruses.

Five of 17 adenoviruses of rhesus or cynomolgus monkey origin induced tumors in newborn hamsters. The tumors appeared between 42 and 280 days after subcutaneous inoculation and had the general characteristics of lymphomas. The tumors were specific by cross-complement fixation tests. An adenovirus recovered from Cercopithecus monkeys appeared to be highly oncogenic; all 23 inoculated hamsters developed tumors within 30 to 40 days.

Adenoviridae↗