Biomedical subjects

C G Edwards

Publications and source records attributed to C G Edwards.

11 recordsLinked to original sources

PubMed2004

Modern diagnostics in chronic myeloproliferative diseases (CMPDs).

According to the new WHO classification a group of chronic myeloproliferative diseases (CMPDs) were defined: chronic myeloid leukemia (CML), chronic neutrophilic leukemia (CNL), chronic eosinophilic leukemia and hypereosinophilic syndrome (CEL/HES), polycythemia vera (PV), chronic idiopathic myelofibrosis (with extramedullary hematopoiesis, CIMF), essential thrombocythemia (ET), and so called CMPD/unclassifiable. As clinical features and laboratory findings differ widely between these diseases several diagnostic approaches are mandatory at diagnosis for classification and are needed also for follow up studies, especially for the measurement of minimal residual disease (MRD). We here outline the laboratory set up at diagnosis and during follow up in CMPDs with specific focus on the respective therapeutical consequences. Only by using a comprehensive diagnostic panel including cytomorphology, cytogenetics, and molecular genetic methods establishing the correct diagnosis, optimizing treatment as well as evaluating treatment response is possible in CMPDs today.

Blood Cell Count↗

PubMed2003

Fermentative activity and production of volatile compounds by Saccharomyces grown in synthetic grape juice media deficient in assimilable nitrogen and/or pantothenic acid.

AIMS: To understand the impact of assimilable nitrogen and pantothenic acid on fermentation rate and synthesis of volatile compounds by Saccharomyces under fermentative conditions. METHODS AND RESULTS: A 2 x 3 factorial experimental design was employed with the concentrations of yeast assimilable nitrogen (YAN) (60 and 250 mg l(-1)) and pantothenic acid (10, 50 and 250 microg l(-1)) as variables. In media containing 250 microg l(-1) pantothenic acid, H2S production by two different species of Saccharomyces decreased when YAN was increased from 60 to 250 mg l(-1). Conversely, H2S production was significantly higher when the concentration of assimilable nitrogen was increased if pantothenic acid was deficient (10 or 50 microg l(-1)). Yeast synthesis of other volatile compounds were impacted by both assimilable nitrogen and pantothenic acid. CONCLUSIONS: While growth and fermentative rate of Saccharomyces was more influenced by nitrogen than by pantothenic acid, complicated interactions exist between these nutrients that affect the synthesis of volatile compounds including H2S. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has important implications for the winemaking industry where a better understanding of the nutritional requirements of Saccharomyces is necessary to reduce fermentation problems and to improve final product quality.

Alcohols↗

PubMed2003

Nonstarter lactic acid bacteria and aging temperature affect calcium lactate crystallization in cheddar cheese.

The occurrence of unappetizing calcium lactate crystals in Cheddar cheese is a challenge and expense to manufacturers, and this research was designed to understand their origin. It was hypothesized that nonstarter lactic acid bacteria (NSLAB) affect calcium lactate crystallization (CLC) by producing D(-)-lactate. This study was designed to understand the effect of NSLAB growth and aging temperature on CLC. Cheeses were made from milk inoculated with Lactococcus lactis starter culture, with or without Lactobacillus curvatus or L. helveticus WSU19 adjunct cultures. Cheeses were aged at 4 or 13 degrees C for 28 d, then half of the cheeses from 4 and 13 degrees C were transferred to 13 and 4 degrees C, respectively, for the remainder of aging. The form of lactate in cheeses without adjunct culture or with L. helveticus WSU19 was predominantly L(+)-lactate (> 95%, wt/wt), and crystals were not observed within 70 d. While initial lactate in cheeses containingL. curvatus was only L(+)-lactate, the concentration of D(-)-lactate increased during aging. After 28 d, a racemic mixture of D/L-lactate was measured in cheeses containing L. curvatus; at the same time, CLC was observed. The earliest and most extensive CLC occurred on cheeses aged at 13 degrees C for 28 d then transferred to 4 degrees C. These results showed that production of D(-)-lactate by NSLAB, and aging temperature affect CLC in maturing Cheddar cheese.

Calcium Compounds↗

PubMed2000

Lactobacillus nagelii sp. nov., an organism isolated from a partially fermented wine.

A Gram-positive rod was isolated from a commercial grape wine undergoing a sluggish/stuck alcoholic fermentation. The organism produced DL-lactic acid from glucose without gas formation, produced dextran from sucrose, hydrolysed aesculin and fermented galactose, D-glucose, D-fructose, D-mannose, L-sorbose, rhamnose, mannitol, sorbitol, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, salicin, cellobiose, maltose, sucrose, trehalose and beta-gentiobiose. 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a distinct subline within the Lactobacillus casei cluster of species. On the basis of phenotypic and phylogenetic evidence, Lactobacillus nagelii sp. nov. ATCC 700692T is proposed as a new species.

Carbohydrate Metabolism↗

PubMed1998

Lactobacillus kunkeei sp. nov.: a spoilage organism associated with grape juice fermentations.

A Gram-positive rod was isolated from a commercial grape wine undergoing a sluggish/stuck alcoholic fermentation. The organism produced L-lactic acid from glucose, possessed weak catalase activity, and fermented relatively few carbohydrates, i.e. glucose, fructose, sucrose, raffinose (weakly) and mannitol. The 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a distinct subline within the Lact. casei cluster of species. Based on phenotypic and phylogenetic evidence, a new species is proposed, Lact. kunkeei. The type strain of Lact. kunkeei is ATCC 700308.

DNA, Bacterial↗

PubMed1990

Thresholds for short-latency auditory-evoked potentials to tones in notched noise in normal-hearing and hearing-impaired subjects.

The thresholds for the short-latency auditory evoked potentials (SLAEPs) to short-duration tones presented in notched-noise masking were evaluated in 20 normal-hearing and 20 hearing-impaired subjects. The differences (dB) between these thresholds (dB nHL) and the pure-tone behavioral thresholds (dB HL) across all 40 subjects were 11.6, 6.1, 6.3 and 0.8 dB for 500, 1,000, 2,000 and 4,000 Hz, respectively. These differences were significantly smaller for the hearing-impaired subjects than for the normal-hearing subjects. Ninety-eight percent of the SLAEP threshold estimations were within 30 dB of the subjects' pure-tone behavioral thresholds and 91% were within 20 dB.

Adolescent↗

PubMed1989

Inducing malolactic fermentation in wines.

Malolactic fermentation (MLF) in wine can be accomplished by relying on the natural microflora or by inducing through inoculation of a specific strain(s) of malolactic bacteria, primarily strains of Leuconostoc oenos. Problems with inducing MLF include intrinsic factors of the grape must such as pH, presence of sulfur dioxide, and ethanol in addition to antagonism of malolactic bacteria by wine yeast. Current methods and new technology to improve the predictability of MLF are discussed.

Journal Article↗

PubMed1987

Brainstem electric-response audiometry in infants of a neonatal intensive care unit.

Brainstem electric-response audiometry was used to assess the auditory function of 600 infants from a neonatal intensive care unit. Two groups of children were tested, one group as inpatients prior to discharge with a mean age at test of 39.4 weeks and one group as outpatients with a mean age at test of 55.4 weeks. Our results indicate that testing infants when they are older will reduce the incidence of failure on the initial evaluation and will reduce the identification of transient hearing losses which resolve spontaneously. Our results also indicate that a threshold of 30 dB nHL or less is probably normal and that some infants with a threshold of 40 dB nHL at first test require otological or audiological management.

Audiometry, Evoked Response↗

PubMed1985

Auditory brainstem responses to clicks in neonates.

Normative data are required when interpreting brainstem electric response audiometry results. An abnormal auditory brainstem response (ABR) cannot be identified without knowledge of the normal ABR and the variability associated with technical and physiological factors. Normative data obtained from babies in a neonatal intensive care unit in response to click stimulation are presented in this paper.

Acoustic Stimulation↗

PubMed1985

Auditory brainstem response audiometry in neonatal hydrocephalus.

Sixteen hydrocephalic NICU babies and 16 age-matched, non-hydrocephalic NICU babies were tested with BERA. The latency and amplitude of waves I and V were compared between groups. Both waves were longer in latency and smaller in amplitude in the hydrocephalic group. The I-V latency interval was within normal limits, but the V/I amplitude ratio was significantly smaller. The reduced amplitude of wave V was the most noticeable ABR abnormality. A total of 11 hydrocephalic babies showed responses that were considered abnormal in amplitude, with all but one having an elevated ABR threshold in at least one ear. These threshold elevations may reflect a neurological condition rather than a peripheral hearing loss.

Audiometry, Evoked Response↗

PubMedDate not supplied

Neonatal auditory brain stem responses from ipsilateral and contralateral recording montages.

Ipsilateral and contralateral auditory brain stem responses were recorded from both ears of 10 neonates between 37 and 43 weeks from conception with normal auditory brain stem function. The most prominent component of the contralateral response was a forehead positive deflection occurring 0.3 millisecond before the ipsilateral wave III followed by two forehead negative waves, the latter present at the same latency as the ipsilateral wave V. The amplitude of the contralateral response was small: at 30 dB nHL only 7 of the 20 ears showed clear responses from this montage. The morphology of the neonatal contralateral response is thus considerably different than the ipsilateral, unlike the adult. A neonate could be mistakenly diagnosed as hearing impaired or neurologically involved if the interpretation is based on the contralateral recording.

Audiometry, Evoked Response↗