Biomedical subjects

C G Kelly

Publications and source records attributed to C G Kelly.

38 records · Page 3Linked to original sources

PubMedDate not supplied

Dissociation of antibody responses during human schistosomiasis and evidence for enhancement of granuloma size by anti-carbohydrate IgM.

Immunoglobulin (Ig) G and IgM antibody levels to soluble egg antigens (SEA), adult worm glycoproteins (AWGP), carbohydrate antigens (CHO) and cationic exchange fraction 6 (CEF6) were measured in serum specimens taken from Brazilian patients with acute, intestinal, hepato-intestinal and hepatosplenic schistosomiasis mansoni. The antibody levels varied among the groups, with the highest anti-egg antigen responses in the acute patients and the highest anti-adult worm responses in patients with chronic disease. The responses to the component parts of the egg antigens were dissociated, with anti-carbohydrate IgG and IgM responses being highest in the acute infection group and anti-CEF6 IgG responses being uniform among the clinical groups. The possibility of a direct role for anti-CHO antibody responses in egg-induced pathology was investigated using the mouse lung model. The anti-carbohydrate monoclonal antibody NIMP/M45 significantly enhanced granuloma formation. Mice given NIMP/M45 produced granulomas larger than those of naive mice or mice given an unrelated monoclonal antibody, and as large as those produced by mice which had been presensitized to egg antigens. The independent regulation of responses to egg antigens may indicate that such responses are minimized to reduce the pathological consequences of infection whilst allowing the development of protective anti-worm responses.

Acute Disease↗

PubMedDate not supplied

Effect of route of immunisation and adjuvant on T and B cell epitope recognition within a streptococcal antigen.

Oral immunisation may elicit both systemic and mucosal immunity. Antibodies directed to a portion (residues 816-1213) of a cell surface adhesin termed streptococcal antigen I/II (SA I/II) of Streptococcus mutans prevent colonisation of this bacterium in vivo. This polypeptide is highly immunogenic in mice and is immunodominant in naturally sensitised humans. In this study, the effects of immunisation by different routes and of adjuvant on T and B cell epitope recognition were investigated. The recombinant polypeptide comprising residues 816-1213 of SA I/II was administered to groups of SJL mice intraperitoneally, subcutaneously or orally. For systemic immunisation, incomplete Freund's adjuvant was used, whereas for oral immunisation the antigen was coupled to the cholera toxin B subunit. The hierarchy of T and B cell epitope recognition differed significantly following different routes of immunisation. These differences in T and B cell responses may be accounted for by extracellular protease activity and processing by antigen presenting cells at the sites of immunisation. Furthermore, epitope recognition may be critical if the immune response elicited by a vaccine must be directed specifically to functional determinants within an antigen. This study emphasises the importance of route of immunisation in vaccine development.

Adjuvants, Immunologic↗