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C Gill

Publications and source records attributed to C Gill.

12 recordsLinked to original sources
PubMed2025

Molecular characterisation of a novel citrus-infecting emaravirus, citrus emaravirus 1.

The application of high-throughput sequencing (HTS) has accelerated the discovery of novel viruses and the genome sequencing of poorly characterised viruses in fruit crops, revealing a greater complexity of plant viromes than previously understood. Here, we report the identification and genomic characterisation of a novel emaravirus, tentatively named "citrus emaravirus 1" (CiEV1), from Citrus limon leaves collected in the North West Province of South Africa. HTS analysis identified contigs corresponding to the four conserved emaravirus genomic segments (RNA1-RNA4), each encoding characteristic proteins: RNA-dependent RNA polymerase, glycoprotein, nucleocapsid, and movement protein. Comparison of terminal sequences and phylogenetic analysis suggest placing CiEV1 within clade D of the genus Emaravirus (family Fimoviridae). To date, no association with symptoms has been identified. This is the first report of an emaravirus detected in citrus, expanding the known host range of the genus and contributing to the growing evidence of viral diversity in citrus. Further investigation is required to determine the epidemiological significance of CiEV1 in citrus orchards.

Citrus↗
PubMed1992

The Cambridge Project for Later Life: design and preliminary results.

The Cambridge Project for Later Life is the follow-up at 2.4 years of the Hughes Hall Project for Later Life, a prevalence study of dementia in Cambridge city in which 40% of the population aged 75 and over were screened for dementia. In the follow-up, 1,173 people were screened a second time, and using Mini-Mental Scale Examination scores were selected for a more intensive interview with CAMDEX. This was followed by detailed neuropsychological testing and magnetic resonance imaging in a smaller proportion of individuals.

Aged↗
PubMed1992

Methodological issues in screening for dementia.

Screening for dementia in populations presents particular difficulties for researchers. In the absence of gold standards for diagnosis, the methods used must be determined by the purposes of the study. In two-stage epidemiological study the screening wave and the diagnostic instrument should be considered together in relation to a third proxy gold standard such as progression of the disorder to moderate and greater severity and neuropathological diagnosis. This provides a measure of the predictive performance of the original screening method and its diagnostic phase. To reduce the variance of estimates of prevalence and incidence it is suggested that the screening interview be a subset of the diagnostic interview.

Aged↗
PubMed1990

Dietary factors and breast-cancer risk in Denmark.

The influence of dietary factors, in particular the intake of fat and beta-carotene, on breast-cancer risk was evaluated in a case-control study including 1,486 breast cancer cases diagnosed over a 1 year period in Denmark. The control group was an age-stratified random sample of 1,336 women from the general population. Data on usual diet prior to the breast cancer diagnosis were collected by self-administered questionnaires of the semi-quantitative food frequency type. A highly significant trend (p less than 0.001) of increasing risk was observed with increasing fat intake, the RR for the highest quartile being 1.45 (95% Cl 1.17-1.80) compared with the lowest. However, information was not available to allow adjustment for the possible confounding effect of energy intake. The risk of breast cancer was not associated with consumption of vegetables rich in beta-carotene, multi-vitamin tablets or other dietary supplements, coffee, tea, sugar or artificial sweeteners.

Adult↗
PubMed1989

Preexcitation syndromes: surgical ablation therapy. The Cleveland Clinic experience.

Thirty-four patients with preexcitation syndrome had surgical ablation of the accessory atrioventricular connection. All presented with sustained symptomatic arrhythmias, which had resulted in syncope in seven patients and aborted sudden cardiac death in four. Arrhythmias induced at electrophysiologic evaluation included orthodromic reciprocating tachycardia in 32 patients, antidromic reciprocating tachycardia in five patients, and atrial fibrillation in 29 patients. A single accessory atrioventricular connection was located in 32 patients and two patients had multiple accessory connections. The accessory connection was located with intraoperative mapping in all patients and the pathway was successfully ablated in 32. Eleven patients underwent a transmural approach and 23 patients underwent an epicardial dissection. Cryotherapy was used in 22 patients. After a mean follow-up of 32 months, 28 patients were free from all arrhythmias without drug therapy. Six patients continued to have symptomatic arrhythmias but only one case was suspected to be secondary to unsuccessful ablation of the accessory connection. One patient with heart block induced at the surgical procedure is dependent upon a pacemaker.

Adolescent↗
PubMed1989

L-658,310, a new injectable cephalosporin. I. In vitro antibacterial properties.

The in vitro antibacterial spectrum of L-658,310, a new semisynthetic cephalosporin, was compared with ceftazidime, aztreonam and piperacillin against a wide variety of randomly selected human clinical isolates. The compound was found to be a broad spectrum bactericidal agent that was more potent than any of the comparison drugs against glucose nonfermenting bacteria. It has especially potent activity against Pseudomonas aeruginosa including multiply-resistant strains. The superior activity of L-658,310 against this group of organisms is attributed to the presence of the dihydroxy substituents on the 2-methylisoindoline moiety of the compound. L-658,310 is not cross-resistant with either imipenem, ceftazidime or piperacillin (representatives of three different classes of beta-lactam compounds) against P. aeruginosa. The lack of cross-resistance with ceftazidime extends to other glucose nonfermenters and several strains of Enterobacteriaceae as well. The compound is active against bacteria known to possess either R-plasmid- or chromosomally-mediated beta-lactamases.

Aerobiosis↗
PubMed1979

Lymphocyte-mediated cytotoxicity microassay by cytofluorography.

A cytofluorometric method for measuring lymphocyte-mediated cytotoxicity is described which employs acridine orange and ethidium bromide stains. These stains permits the rapid (approximately 45 sec per sample) differential enumeration of viable cells and are therefore valuable in the preparation and performance of a cytotoxicity assay. Reproducibility of viable target cell counts is good and, statistically, the same mean result is obtained when the cytofluorometric and an accepted radioisotopic method are compared. It is found that the method employing radioisotopic label ([125I]iododeoxyuridine) non-specifically killed 80% of the target cells, whereas the cytofluorographic method does not result in non-specific kill. Therefore, the cytofluorometric method reflects in vivo conditions more accurately. The cytofluorometric method also provides a more rapid and less technically demanding assay than those previously described.

Animals↗
PubMed1976

Quantitation of "acute-phase proteins" postoperatively. Value in detection and monitoring of complications.

Serial determinations of serum C-reactive protein are helpful in the detection and monitoring of postoperative complications associated with inflammation and/or tissue necrosis. The serum C-reactive protein level begins to increase within sex hours after operation, peaks on the second day, and by the third postoperative day begins to decrease toward the preoperative level. In cases with surgical complications involving inflammation, serum C-reactive protein levels remain elevated and do not show a decline on the third postoperative day. Serum levels of other "acute-phase proteins," such as alpha-1 acid glycoprotein, ceruloplasmin, alpha-1 antitrypsin, and haptoglobin, were found to increase in response to surgical procedures, but subsequent to the increase in C-reactive protein. These other proteins offer no additional information in monitoring the postoperative acutephase response,

Abscess↗
PubMedDate not supplied

Lymphocyte blastoid transformation assay by cytofluorography.

This rapid, automated, micromethod quantitates blastoid transformation using a supra vital stain, acridine orange, in quantitating the ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) before and after cell stimulation. Only 0.2 ml of culture containing 1.0 X 10(5) to 1.5 X 10(5) lymphocytes is required to obtain results every 5 minutes. These results include a total cell count and cell counts of the populations with increased RNA (I-RNA), and increased DNA (I-DNA). The I-RNA and I-DNA lymphocyte populations, as determined by the cytofluorometric method, correlate well with the accepted H3 uridine and C14 thymidine uptake methodology. The phytohemagglutinin dose response measurements by the 2 methods also correlate well. Blast cell counts, by Wright-Giemsa staining, showed near identity to the I-RNA cell population.

Blood Cell Count↗