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Biomedical subjects

C Glass

Publications and source records attributed to C Glass.

8 recordsLinked to original sources
PubMed1991

Preterm premature rupture of membranes: detection of infection.

This prospective study was designed to determine the value of a daily modified biophysical profile in detecting infection in patients with preterm premature rupture of the membranes who were managed expectantly. Ninety-nine patients received daily nonstress tests and biophysical profile scores. Results of the last predelivery study were related to subsequent development of amnionitis or fetal sepsis. Infection was present in 16 patients. When the biophysical profile score was 0/8, infection was uniformly present. When fetal breathing was absent (biophysical profile score, less than or equal to 4/8) and nonstress test was nonreactive, infection was present in 75% of cases (sensitivity, 75%; specificity, 95%). Because a nonreactive nonstress test could be secondary to prematurity instead of infection, these results were analyzed over time. Those who initially had a reactive nonstress test that subsequently became nonreactive were more likely to be infected. We conclude that a daily biophysical profile score and nonstress test can detect infection and propose delivery of patients with a biophysical profile score of 0/8 and nonreactive nonstress test. Patients with absent fetal breathing and a nonstress test that changes from reactive to nonreactive also should be considered for delivery. Absent fetal breathing with a reactive nonstress test or a consistently nonreactive nonstress test should have further testing to rule out infection.

Bacterial Infections↗
PubMed1988

Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells.

Simple epithelial cells synthesize a different set of keratins than epidermal cells. In experiments reported in this manuscript, we show that the base level of keratin expression in simple epithelial cells is variable for different cell types, and that, in some simple epithelia, this level can be upregulated by increasing the exposure of cells to retinoids, but not glucocorticoids or estradiol. To elucidate the molecular mechanisms underlying simple epithelial keratin gene regulation, we have isolated and characterized a human gene encoding the simple epithelial keratin K7. By examining the possible regulatory elements of this gene and by investigating the behavior of this gene introduced transiently into simple epithelial cells, we have uncovered a possible basis for the differential expression of epidermal and simple epithelial keratin genes.

Amino Acid Sequence↗
PubMed1987

A functional analysis of recidivistic arson.

Evidence from studies of pathological arson and normal fire setting as well as the authors' clinical experience with arsonists in a maximum security hospital is employed to generate a model of recidivistic arson within the conceptual framework of functional analysis. It is contended that certain psychosocial stimuli, in the context of major setting conditions, predispose the individual towards incendiarism which is initially positively and negatively reinforced. However, the longer-term consequences of arson are considered to maintain and exacerbate the antecedent problems encountered by arsonists. Specifically, arson is viewed as an attempt to exert a change in the arsonist's life conditions where alternative behaviours have proved, or are perceived to be, ineffective. The model incorporates adaptations of the displaced aggression and arousal hypotheses of arson, and examines possible developmental aspects of pathological arson from normal childhood fire play, and suggests that a transition from fire setting in the company of others to incendiarism alone constitutes a major factor in the pathological process. The social, clinical and theoretical implications of the model are discussed with reference to current management and treatment strategies as well as future research.

Arousal↗
PubMed1986

Synchronized synthesis and intracellular transport of serum albumin and apolipoprotein B in cultured rat hepatocytes as studied by double immunofluorescence.

Synthesis and intracellular transport of two secretory proteins, serum albumin (SA) and apolipoprotein B (apo B) have been synchronized in primary cultures of normal rat hepatocytes to make possible immunocytochemical study of the transport pathway. Under appropriate conditions of cycloheximide treatment, synthesis of new protein was inhibited and, by double immunofluorescent labeling, the cells were found to be largely depleted of the SA and apo B previously synthesized. Re-initiation of protein synthesis led to sequential appearance of SA and apo B, first in the endoplasmic reticulum, then in the Golgi complex, and finally at the cell surface. These results indicate that it should be feasible to use this cell system for high-resolution investigation of the sequence of structures involved in intracellular transport of SA and apo B by corresponding immunolabeling experiments as observed by electron microscopy.

Animals↗
PubMed1985

Uptake of high-density lipoprotein-associated apoprotein A-I and cholesterol esters by 16 tissues of the rat in vivo and by adrenal cells and hepatocytes in vitro.

The uptake of high-density lipoprotein (HDL)-associated apolipoprotein A-I and cholesterol esters was estimated in 16 tissues of the rat using rat HDL doubly labeled with nondegradable tracers; covalently attached 125I-tyramine-cellobiose traced apo-A-I, and [3H]cholesteryl linoleyl ether traced cholesterol esters. Both labels remained associated with the HDL fraction in the plasma, adequately traced their unlabeled counterparts, and were well trapped at their sites of uptake. Cholesteryl ether was taken up at a greater fractional rate than apo-A-I by adrenal, ovary, and liver: 7-fold, 4-fold, and 2-fold greater, respectively. The rates of uptake of cholesteryl ether and apo-A-I were about equal in the other tissues (except kidney). The disproportionate uptake of HDL cholesteryl ether relative to HDL apo-A-I was also observed in primary cultures of rat adrenal cells and hepatocytes. Uptake of both moieties in both cell types showed saturability. Both the absolute rate of uptake of [3H]cholesteryl ether and the ratio of ether uptake to apo-A-I uptake were greater in adrenal cells than in hepatocytes, consonant with the in vivo observations. Very similar results were obtained using HDL biologically labeled with [3H]cholesterol esters. The disproportionate uptake of [3H]cholesteryl ether was not significantly decreased by depletion of apo-E from the HDL nor by reductive methylation of the apo-E to block its recognition by receptors. However, apo-A-I uptake was decreased, suggesting that apo-E mediates the uptake of particles containing apo-A-I but does not contribute to the disproportionate uptake of [3H]cholesteryl ether.

Adrenal Glands↗
PubMed1985

Familiarity, spatial frequency and task determinants in processing laterally presented representations of faces.

Laterally presented portraits were judged as familiar or unfamiliar (Experiments 1 and 2), or were processed in a target matching task (Experiment 3). Exposure durations (100 or 190 msec.) were varied between (Experiments 1 and 2) or within (Experiment 3) the experiments. Clear and degraded faces appeared either randomly intermingled (Experiment 1) or in blocked sequences (Experiments 2 and 3). In Experiment 3 familiar and unfamiliar stimuli also appeared in blocks. Stimulus degradation was achieved by coarse quantization of the image into blocks, so replacing relevant high-spatial-frequencies (the features) by spurious information, preserving only the original lower-spatial-frequency components. Left hemisphere mediation was strongest and most consistent with long exposures and random sequences of clear and degraded stimuli. Right hemisphere mediation tended to appear with shorter exposures and degraded stimuli presented in blocks. Though the interactions were often complex, the general pattern of results was consistent with the analytic-holistic processing dichotomy.

Discrimination Learning↗
PubMed1985

Sequence and expression of a human type II mesothelial keratin.

Using mRNA from cultured human mesothelial cells, we constructed bacterial plasmids and lambda phage vectors that contained cDNA sequences specific for the keratins expressed in these cells. A cloned cDNA encoding keratin K7 (55 kD) was identified by positive hybrid selection. Southern Blot analysis indicated that this sequence is represented only once in the human genome, and Northern Blot analysis demonstrated that the gene encoding K7 is expressed in abundance in cultured bronchial and mesothelial cells, but only weakly in cultured epidermal cells and not at all in liver, colon, or exocervical tissue. The predicted amino acid sequence of this keratin has revealed a striking difference between this keratin and the type II keratins expressed in epidermal cells: whereas all of the epidermal type II keratins thus far sequenced have long nonhelical termini rich in glycine and serine, this mesothelial type II keratin has amino and carboxy terminal regions that are unusually short and lack the inexact repeats of glycine and serine residues.

Amino Acid Sequence↗