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C Granberg

Publications and source records attributed to C Granberg.

10 recordsLinked to original sources
PubMed1988

Determination of cytotoxic T lymphocyte activity by time-resolved fluorometry using europium-labelled concanavalin A-stimulated cells as targets.

Time-resolved fluorometry was used to detect the cytolysis of concanavalin A-stimulated blast cells by employing cells stimulated in a mixed lymphocyte culture as effectors. The target cells were labelled with europium diethylenetriaminopentaacetate (EuDTPA) chelates. The results obtained showed that this method, which has been successfully applied to the measurement of natural killer cell activity, was also applicable to the more specific cytotoxic T lymphocyte reaction. The specific release was higher with EuDTPA-labelled target cells than with 51chromium-labelled target cells. As with 51chromium, EuDTPA can be used to distinguish between the cytolysis of specific and non-specific target cells.

Chromium Radioisotopes↗
PubMed1986

Europium-labelled target cells in an assay of natural killer cell activity. II. A novel non-radioactive method based on time-resolved fluorescence. significance and specificity of the method.

The significance, specificity and high sensitivity of a new method to determine the natural killer cell cytolysis of europium diethylenetriaminepentaacetate (EuDTPA)-labelled target cells has been confirmed. The targets used in this release assay were the NK sensitive cell line K-562 and the resistant cell line Raji. The released EuDTPA was detected by a method based on time-resolved fluorometry. The specific EuDTPA release was higher than specific 51chromium (51Cr) release. Competitive assays, where half of the target cells were labelled with EuDTPA and the other half with 51Cr and the use of double labelled target cells showed that the results were identical with those of single labelled cells. The reliability of the EuDTPA release assay was further confirmed by performing experiments using NK cells from a patient whose complete lack of NK activity had earlier been demonstrated with the 51Cr release assay. Furthermore, our studies show that the amount of incorporated EuDTPA was directly proportional to the concentration of marker used. Due to the proportional incorporation of EuDTPA the labelling conditions can be chosen to obtain a sensitivity which allows even single cells to be detected.

Cells, Cultured↗
PubMed1986

Europium-labelled target cells in an assay of natural killer cell activity. I. A novel non-radioactive method based on time-resolved fluorescence.

The use of a new marker for labelling cells used as targets for natural killer cells is described. The human erythroleukaemic cell line K-562 was used as target. The cells were labelled with europium diethylenetriaminopentaacetate (EuDTPA) chelates. The detection of the released marker is based on time-resolved fluorometry. The results obtained show that the method is sensitive, specific and rapid. The high specific activity of the marker and the sensitivity of the detection apparatus result in numeric values (counts per second) which are 10-20 times higher than the values (counts per minute) obtained with 51chromium. In comparison with 51chromium release assay the labelling of target cells is less time consuming, the marker release more rapid and the detection time of released marker only 1 second per tube. The use of this non-radioactive marker is an alternative way of measuring natural killer cell mediated cytolysis of target cells.

Cell Line↗
PubMed1985

Concanavalin A induced and spontaneous suppressor cell capacity of newborn and fetal lamb lymphocytes.

Concanavalin A induced newborn lamb blood lymphocytes suppress the proliferation of adult lymphocytes to the same magnitude as similarly induced adult suppressor cells. Concanavalin A induced suppressor capacity of fetal spleen cells at term is not as strong as that of newborn peripheral blood lymphocytes. Thymocytes of the same fetuses do not possess any suppressive capacity inducible by this mitogen, but show a spontaneous suppressor capability. Spleen cells exhibit only a low spontaneous activity. Interestingly, when peripheral blood lymphocytes of newborn lambs spontaneously suppress the response of both unrelated and maternal cells, adult lymphocytes enhance the proliferation. These results show that functional suppressor cells are found in both newborn and fetal lambs. Our data also indicate that cells spontaneously suppressive are established in thymus and spleen, whereas cells that can be rendered suppressive by concanavalin A stimulation are found in the spleen. Therefore, the spontaneous thymic suppressor cells may represent a more primitive cell population than those of the spleen.

Animals↗
PubMed1979

Cell-mediated lympholysis by human maternal and neonatal lymphocytes: mother's reactivity against neonatal cells and vice versa.

Maternal reactivity in cell-mediated lympholysis (CML) against cells from her own child is, in average, half of the maternal reactivity against unrelated adult cells. This finding remains the same when cells from a newborn or from an older child are used, suggesting that the reduced maternal reactivity is based rather on the one haplotype identity between the mother and child than on the occurrence of specific maternal tolerance. Consistently, CML-capacity of the child, directed against cells of own mother, is half of the control values, again independently of the child's age.

Adolescent↗
PubMedDate not supplied

Activity of natural killer cells after postoperative amino acid infusion.

Effects of postoperative infusion of a 3% amino acid solution on natural killer (NK) cell activity were studied in 10 patients undergoing major abdominal surgery. Eight additional patients formed the control group. Preoperative NK cell activity against K562 target cells determined in a short-term 51Cr release assay was similar in patients of both groups. After the operation, in general the patients of both groups showed a decrease in NK cell activity (p less than 0.01) but without differences between the groups. There were no changes in the percentages of Leu-7 positive cells (NK cells) between or within the two groups. However, the number of Leu-7 positive cells in blood was lower in the amino acid than in the control group patients during the postoperative days 3-7 (p less than 0.05 and 0.01). In vitro, the amino acid solution proved to be nontoxic both for effector and target cells and no depression of NK cell activity was observed. A 16-hr preincubation of mononuclear cells with the amino acid solution did not affect NK cell activity. The results suggest that postoperative infusion of an isotonic amino acid solution does not routinely offer any advantages over glucose containing electrolyte solution as to its effects on NK cell activity.

Adult↗