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Biomedical subjects

C Grassi

Publications and source records attributed to C Grassi.

At least 127 records · Page 7Linked to original sources
PubMed1975

Ethanol-antibiotic interactions at hepatic level.

The authors study the influence of acute and chronic ethanol intoxication on the blood levels of rifampicin and isoniazid as well of aminoglycoside antibiotics in rats. While the acute ethanol intoxication has no influence on blood levels of tested antibiotics, in the chronic inotoxication lower levels of rifampicin and higher levels of isoniazid are observed. A study was performed also to determine the influence of an acute and chronic treatment with alcohol and the avoe quoted antibiotics on the BSP elimination rate. In the acute as well as in the chronic experiments, a constant significant delay in the elimination of BSP was observed in animals treated with rafampicin either alone or associated with alcohol. Isoniazid modified the dye elimination in comparison to controls only in chronic experiments. Ethanol did not seem to have any influence on this behavior. The implications and the possible significance of these findings are discussed.

Acute Disease↗
PubMedDate not supplied

Immunohistochemical distribution of mucinous-like carcinoma associated antigen (MCA) in breast and non-breast cancer: comparison with other biological parameters.

The present study reports the immunohistochemical reactivity of the monoclonal antibody b-12 (MAb b-12) with malignant human tissues. 173 neoplastic tissues were tested: MAb b-12 stained all breast carcinomas independently of their histology, with different patterns within the various type of cancer. Some other carcinomas (stomach, bowel, ovary, lung, endometrium), were also reactive even if the fraction of positive cells was lower. A comparison between the histological localization of MCA and that of CEA was performed; anti-CEA antibodies stained the cancer tissues with different reactivity and showed different percentages of positivity. MCA expression was also compared with other biological parameters such as the presence of estrogen receptors (ER), progesterone receptors (PgR), epithelial growth factor receptors (EGF-R), and oncoprotein p-53 which is encoded by the oncogene N-myc. The proliferative activity was also evaluated by measuring the growth fraction (GF) using the antibody Ki67. Any correlation was demonstrated between MCA and these parameters except for growth fraction as revealed by Ki67 antibody.

Antibodies, Monoclonal↗
PubMedDate not supplied

Demonstration of mucinous-like carcinoma-associated antigen in bone marrow and lymph node biopsies from patients with breast carcinoma.

Two hundred and fifty bone marrow and 140 lymph nodal biopsies were analyzed immunocytochemically, using a mouse monoclonal antibody b-12 (M Ab b-12), which reacts with MCA (mucinous-like carcinoma-associated antigen). The presence of MCA in bone marrow specimens was demonstrated in 102 out of 105 (97.1%) breast cancer metastases, 5 out of 8 (62.5%) gastric cancers, 5 out of 6 (83.3%) colon cancers, 3 out of 5 (60%) prostate cancers, 11 out of 26 (42.3%) lung cancers and 25 out of 30 (83.3%) unknown primary cancers, while no positivity to anti-MCA antibody was found in 30 cases of normal bone marrow biopsies, 5 cases of non epithelial malignancies and 30 cases of hemolymphoproliferative disease. Normal lymph nodes and non-epithelial lymph node metastases did not show any reaction to M Ab b-12; on the contrary MCA positive staining was observed in 75 out of 75 (100%) lymph nodal metastases in breast cancer. These results suggest that application of M Ab b-12 in immunohistochemistry is valid for the detection of bone marrow and lymph nodal micrometastases of epithelial origin.

Antibodies, Monoclonal↗