Biomedical subjects

C Gris

Publications and source records attributed to C Gris.

3 recordsLinked to original sources

PubMed1989

Complexity, polymorphism, and recombination of mouse T-cell receptor alpha gene families.

Genomic DNA from a large panel of inbred strains of mice were hybridized sequentially with 15 V alpha, 2 V delta, 1 C alpha, and 1 C delta probes. Most of the V alpha probes detected a high degree of polymorphism and have allowed the definition of five mouse T-cell receptor alpha (Tcr alpha) haplotypes. One of these haplotypes (Tcre alpha) appears to arise from a recombination between the Tcrb alpha and Tcra alpha haplotypes, the latter being the most frequently found in the conventional inbred strains. This recombination event clearly indicates that the members of at least 11 V alpha sub-families are not closely linked but highly interspersed with one another on chromosome 14.

Animals↗

PubMed1986

Serologic variations in kappa light chains of various Mus species correlate with differences in gene structure.

Kappa light chains were investigated in sera of several groups of wild derived mouse strains by radioimmunological analysis using xenogeneic anti-mouse kappa light chain antibodies. Although the majority of strains tested expressed serologically indistinguishable kappa chains, several significant exceptions were observed. Anti-kappa chain reactivity was not detectable in normal sera of Mus caroli and Mus cervicolor species using specific antiserum. These strains express a variant of the kappa light chain. Some serologic variation was also observed in kappa chains from members of the subgenus Nannomys. Southern blot analyses using C kappa and J kappa probes indicated different patterns of hybridization in strains that were serologically distinguishable. Mus caroli and Mus cervicolor DNAs digested with the enzymes EcoRI or BamHI show variation from BALB/c and most other mouse strains tested in the size of the hybridizing fragments. This correlation between serologic and blotting analyses is no longer found in SJL and SPE strains, which show variation in BamHI cleavage but not at the serological level. The majority of laboratory and wild derived mice analyzed have the same pattern of hybridization. Taken together our results may also have implications for the phylogenetic classification of wild type mouse strains.

Animals↗

PubMed1985

Lambda light chain constant and variable gene complements in wild-derived inbred mouse strains.

Previous investigations of lambda light (L) chains in wild-derived inbred (WDI) mouse strains revealed large variations in serum levels of this L chain type as well as differences in antibody responses in which lambda chains predominate. In the present study a diverse group of WDI strains was analyzed by Southern blot analysis using probes for V lambda, C lambda 1 and C lambda 2 genes in an attempt to correlate the complement of lambda genes present in these strains with their lambda expression and with their taxonomic classification. All strains studied had two or three DNA sequences that strongly hybridized with a V lambda probe derived from BALB/c; most strains had V lambda sequences on restriction fragments similar in size to V lambda 1 and V lambda 2 sequences from BALB/c. Comparison of C lambda 1 and C lambda 2 genes in WDI mice to those of BALB/c revealed extensive variation both in number of hybridizing fragments and in their sizes. There were no obvious correlations observed between C lambda and V lambda patterns obtained for mouse strains of any phylogenetic group suggesting that constant and variable regions of lambda have evolved independently. In contrast to variations found for C lambda, a single kappa constant region gene appears to be conserved throughout the various mouse species.

Animals↗