Biomedical subjects

C H Lo

Publications and source records attributed to C H Lo.

3 recordsLinked to original sources

PubMed1979

Physical properties of human serum hexosaminidases A and B: studies in normal and cancer patients.

The major forms of human serum hexosaminidases A and B (Hex A and Hex B) were isolated from normal subjects and cancer patients using DEAE-cellulose. In normal serum, Hex A was heat-labile and had an apparent KM of 1.13 mM and Vmax of 0.51 mumol/ml/h; Hex B was heat-stable and had an apparent KM of 0.85 mM and Vmax of 0.22 mumol/ml/h. Both forms had the same pH optimum, at 4.3. Hex A and Hex B from the sera of cancer patients resembled their normal counterparts in heat stability, pH optimum, and apparent KM (1.07 mM for Hex A, 0.88 mM for Hex B). In contrast, the Vmax values for the cancer sera isozymes were greater than those of normal sera (0.70 and 0.40 mumol/ml/h for Hex A and Hex B, respectively).

Hexosaminidases↗

PubMed1978

Human serum hexosaminidase: elevated B form isozyme in cancer patients.

The activity of N-acetyl hexosaminidase (beta-2-acetamido-2-deoxy-D-glucoside:N-acetamido-deoxyglucohydrolase, E.C. 3.2.1.30) was significantly greater in sera of patients with solid malignant tumors than in sera of healthy volunteers or of patients with nonmalignant ailments. The increased activity was found in the two major isozymes, hexosaminidase A and hexosaminidase B, but the increase in the latter isozyme was more prominent than in the former. Measurement of the levels of the hexosaminidase isozymes may provide an ancillary diagnostic test for malignancy.

Adolescent↗

PubMedDate not supplied

The effects of metiamide on gastric secretion and stress ulceration in rats.

The effects of metiamide, a histamine H2 blocker, on gastric secretion and ulcer formation in stressed pylorus-occluded rats were investigated. Metiamide, like atropine, significantly reduced the volume of gastric secretion and total acid output in unrestrained pylorus-occluded rats. Both drugs produced greater decreases in the volumes of gastric secretion in stressed rats than in their corresponding unrestrained groups. Stress itself reduced both parameters. Metiamide, like atropine, significantly reduced the incidence of gastric stress ulcers. When given together these two drugs did not provide greater protection. The results obtained with metiamide indicate that histamine plays a role in basal gastric secretion and in the pathogenesis of stress ulcers. As no correlation between gastric acid secretion and ulcer formation was demonstrated in this study, it is suggested that H2 receptors may also be involved in gastric motility. However, the possibility that metiamide could exert its ulcer-protecting effects through other mechanisms cannot yet be excluded.

Animals↗