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Biomedical subjects

C H Tempelis

Publications and source records attributed to C H Tempelis.

At least 37 records · Page 2Linked to original sources
PubMed1978

The requirement for the Fc portion of antibody in antigen-antibody complex-mediated suppression.

The role of the Fc portion of antibody in immune complex-induced suppression was studied in vivo and in vitro. BSA pheasant anti-BSA complexes, formed in antigen excess (Ag1Ab1), were found to suppress both responses to BSA and SRBC. When complexes were formed with F(ab')2 fragments of pheasant anti-BSA, no suppression was observed, indicating that the Fc piece was indeed essential for the induction of Ag-Ab complex-mediated suppression.

Animals↗
PubMed1978

Immunologic tolerance in the chicken. I. Isolation of two suppressive fractions from serum of chickens tolerant to bovine serum albumin.

Serum from 14-day-old chickens rendered tolerant at hatching with 1250 mg bovine serum albumin (BSA)/KBW was found to suppress an adoptive secondary response to BSA and an in vitro response to sheep red blood cells (SRBC). Chromatographic separation resulted in three fractions: I, II, and III. The suppressive activity was found to be contained in fraction I (m.w. greater than 1 X 10(6)) and fraction III (m.w. less than 2.5 X 10(4)). Analysis of fraction I by affinity chromatographic techniques revealed that it was composed of soluble BSA-antibody complexes and their removal removed the suppressive activity. Analysis of peak III revealed that the suppressive activity could only be removed by BSA and anti-Line 3 antiserum columns.

Animals↗
PubMed1977

The role of antigen-antibody complexes in mediating immunologic unresponsiveness in the chicken.

A significant suppression of an adoptive secondary response to either BSA or sheep red blood cells (SRBC) resulted when chicken spleen cells were incubated with BSA-antibody (Ab) complexes formed in antigen excess (molar ratio Ag1Ab1.1). Serum from 14-day old birds rendered unresponsive at hatching with large doses of BSA also produced suppression. In addition the in vitro response to SRBC was suppressed by the above treatment. Complexes formed at equivalence either had no effect on the subsequent responses of primed cells or produced an enhancement. The fact that both the homologous and heterologous responses were inhibited could indicate that both were suppressed via a common pathway.

Animals↗
PubMed1976

Relation of mosquito density to bird: mammal feeding ratios of Culex tarsalis in stable traps.

Stable traps, each baited with a jackrabbit and either a chicken or a pheasant, collected more than 21,000 mosquitoes in the Sacramento Valley, California, in 1972 and 1973. The focus of interest was the feeding behavior of Culex tarsalis, a primary vector of encephalitis viruses. Generally, feeding success was less and feeding rates on the jackrabbit were greater when larger numbers of mosquitoes were collected, or when a bird was exposed that was less receptive to mosquito feeding. Greater feeding rates on the jackrabbit apparently resulted from decreased feeding on the avian host and from diversion of mosquitoes to the jackrabbit. Figures are given that show how changes in feeding rates on birds affect the probabilities that a mosquito that feeds twice would feel both times on a bird, or on a bird and then on a mammal. Relatively small collections of nine mosquito species other than C. tarsalis revealed distinct species differences in feeding behavior. Aedes malanimon showed a preference for jackrabbit, which is relevant to its role as a vector of western equine encephalitis and California encephalitis viruses.

Aedes↗
PubMed1976

Species identification of blood meals from Culex tarsalis that had fed on passeriform birds.

Blood-engorged Culex tarsalis (4,209) collected during 1960 to 1965 in Kern County, California and previously shown to have fed on passeriform birds by the precipitin test were further tested by the more sensitive passive hemagglutination inhibition test (PHI). It was shown that these mosquitoes fed principally on House Finches and House Sparrows, the most common passeriform birds found in the collection areas. These data demonstrate the usefulness of the PHI test for the identification of arthropod blood meals from closely related species.

Animals↗
PubMed1975

Antigen-antibody complexes produced in chickens tolerant to bovine serum albumin.

A radioimmunoassay for circulating soluble antigen-antibody complexes is described. The assay detects complexes either in antigen or antibody excess. Soluble complexes were found in the sera of chickens tolerant to bovine serum albumin (BSA). The complexes appeared in the serum as soon as 7 days following neonatal induction of tolerance. The amount of complexes reached a peak between 1 to 2 weeks of age and disappeared by 6 weeks when responsiveness returned. The complexes were found in the bottom third of a 10 to 40% sucrose density gradient and by analytical ultracentrifugation indicated a size of 22.8S. If tolerant chickens were challenged with BSA at 2, 4 or 6 weeks, the disappearance of complexes was not accelerated, and a proportion of the previously tolerant chickens exhibited a heightened antibody response.

Animals↗