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C H WANG

Publications and source records attributed to C H WANG.

At least 19 recordsLinked to original sources
PubMed1965

PYRUVATE METABOLISM, CARBON DIOXIDE ASSIMILATION, AND NITROGEN FIXATION BY AN ACHROMOBACTER SPECIES.

Hamilton, I. R. (University of Wisconsin, Madison), R. H. Burris, P. W. Wilson, and C. H. Wang. Pyruvate metabolism and carbon dioxide assimilation by an Achromobacter species. J. Bacteriol. 89:647-653. 1965.-Carbon dioxide fixation by washed whole cells of Achromobacter N4-B has been observed during anaerobic pyruvate metabolism with both nitrogen- and NH(4) (+)-grown cells. Labeled sodium bicarbonate-C(14) was assimilated into cells by a mechanism requiring pyruvate under conditions of nitrogen fixation, nitrogenase induction, and assimilation of NH(4) (+). Of the assimilated radioactivity, 89% appeared in six amino acids and two ninhydrin-positive unknown compounds, with the distribution of the label essentially independent of the nitrogen nutritional state of the organism. Aspartic and glutamic acids were the most highly labeled, with lesser amounts in glycine, alanine, ornithine, arginine, and the unknowns. All of the radioactivity extracted from these cells by ethanol-boiling water appeared in a protein fraction precipitated by 20% trichloroacetic acid. Radiorespirometric experiments with individually labeled pyruvate substrates demonstrated the preferential decarboxylation of the C-1 of pyruvate by this organism in a flowing helium gas phase. This decarboxylation was almost completely inhibited by using flowing nitrogen in place of helium; the addition of 0.5% CO(2) to the flowing nitrogen prevented inhibition and allowed 70% of the expected CO(2) evolution. These results, coupled with those from growth experiments, indicate a carbon dioxide requirement for anaerobic growth and pyruvate metabolism, which appears to be coupled to the formation of protein precursors.

Achromobacter↗
PubMed1962

Dissimilation of glucose and gluconic acid by Pseudomonas natriegens.

Eagon, R. G. (University of Georgia, Athens) and C. H. Wang. Dissimilation of glucose and gluconic acid by Pseudomonas natriegens. J. Bacteriol. 83:879-886. 1962-When glucose dissimilation of a marine pseudomonad, Pseudomonas natriegens, was studied, enzymes of both the glycolytic pathway and of the hexose monophosphate pathway were detected in extracts of glucose-grown cells. Enzymes of the Entner-Doudoroff pathway and phosphoketolase were not detected. Data from radiorespirometric experiments indicated that approximately 92 and 8% of glucose actually catabolized were routed via the glycolytic and the hexose monophosphate pathways, respectively. When P. natriegens was induced to utilize gluconate, it was demonstrated that gluconokinase and enzymes of the Entner-Doudoroff pathway were induced. Radiorespirometric experiments with cells under growing conditions revealed that gluconate was dissimilated predominantly (80%) via the Entner-Doudoroff pathway. This observation was in contrast to the observation that the glycolytic pathway is practically the exclusive catabolic pathway for glucose dissimilation. A minor portion of substrate gluconate was also catabolized by this organism via the hexosemonophosphate pathway. However, the pentose phosphate derived from substrate gluconate is believed not to be catabolized extensively.The important facet uncovered by these experiments was the extensive operation of the glycolytic route of glucose dissimilation. This is in contrast to other pseudomonads studied to date, which have been reported to dissimilate glucose predominantly via the Entner-Doudoroff pathway and which do not utilize the glycolytic pathway.

Gluconates↗