Biomedical subjects

C H Wang

Publications and source records attributed to C H Wang.

At least 127 records · Page 7Linked to original sources

PubMed1999

Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single-nucleocapsid baculovirus.

The baculovirus late expression factor 2 (LEF-2) is involved in DNA replication, and most likely function as a primase processivity factor. Lef-2 genes have been found in multinucleocapsid nucleopolyhedroviruses (MNPVs) and in granuloviruses (GVs), but not yet in single-nucleocapsid NPV (SNPV). Here, a lef-2 gene homolog was identified from SNPV of Helicoverpa armigera (HearNPV). The open reading frame of the HearNPV lef-2 gene is 696 nucleotides long, encoding a putative protein of 232 amino acids with an M(r) of about 26 kDa. The 5'-noncoding region contains two early (CAGT) consensus motifs for transcription initiation and three TATA boxes. Lef-2 transcripts started at a C, 29 nucleotides upstream of a putative translational start. A putative polyA signal, AATAAA, was found 76 nucleotides downstream of the translation stop codon. The HearNPV lef-2 gene has a low but significant degree of amino acid sequence identity (30%) to the lef-2 genes of 15 other baculoviruses of which nine were newly determined. The N-terminal half of the LEF-2 proteins contains one (I) and the C-terminal half two (II and III) conserved domains. Sixteen amino acids are absolutely conserved in those LEF-2 investigated and are probably critical for LEF-2 function. A phylogenetic tree of 16 baculovirus LEF-2 proteins was constructed by using maximum parsimony analysis and appeared to be comparable to a tree for ecdysteroid UDP-glucosyl transferases (Chen et al., 1997a). The genomic location of the lef-2 genes relative to polyhedrin/granulin and the clade structure of the gene trees suggest that genome organization and gene phylogeny are useful parameters to study the evolutionary history of baculoviruses. These two independent approaches also give a more complete picture of the ancestral relationship among baculovirus.

Amino Acid Sequence↗

PubMed1999

Left stellate stimulation increases left ventricular ejection fraction in patients with essential palmar hyperhidrosis.

Left stellate stimulation increases cardiac contractility, heart rate, systolic blood pressure, and QT interval in experimental animals. To see if these changes occur in humans, we stimulated the left stellate ganglia with a monopolar coagulation power of 5 W in 10 patients with palmar hyperhidrosis, axillar hyperhidrosis, or both. We also stimulated the right stellate ganglia of the other 10 patients. The mean left ventricular ejection fraction (LVEF, measured with M-mode echocardiography), QT interval, heart rate and systolic blood pressure of the baseline were 54.72%, 403 ms, 65/min, and 115 mmHg, whereas those after 45 s of left stellate stimulation were 62.84%, 434 ms, 73/min, and 123 mmHg respectively. We compared these data with those of the baseline and the two-tailed P values were 0.005 for both LVEF and QT interval, 0.052 for heart rate, and 0.050 for systolic blood pressure respectively (Wilcoxon Matched-Pairs Signed-Ranks Test). The corresponding P-values for those of the right stellate stimulation were 0.721, 0.203, 0.260, and 0.326 respectively. All these suggest that the left stellate ganglia predominate the right ones in affecting LVEF, QT interval, heart rate and systolic blood pressure in humans, that left stellate stimulation increases LVEF and prolongs QT interval significantly, and that left stellate stimulation accelerates heart rate and elevates systolic blood pressure marginally.

Adult↗

PubMed1999

Rapid anti-helminthic response of B lymphocytes in the intestinal mucosal tissues of rats.

B cell response to Trichinella spiralis (Ts) adult antigen (Ag) was studied in rats 1-20 days postinfection. B cell recoveries from the mesenteric lymph node (MLN), Peyer's patches (PP), thoracic duct lymph (TDL), and the spleen were determined by FACS analysis and Ag-specific antibody-producing cells (Ab-pc) in these tissues were enumerated using the immunoplaque assay. Total B cell numbers increased 2-70 times from day 3 postinfection in the MLN and TDL obtained from MLN-resected rats (MX) and such proliferation was not found in the PP or the spleen. Ab-pc of all isotypes increased from day 3 in the MLN and from day 2 in the MX-TDL. Among all isotypes, IgE- and IgG1-pc showed the strongest response. Immunofluorescence study revealed that these B cells were activated in the non-PP region of the small intestine. These results indicate an early isotype switch to IgG1 and IgE production in Ts-infected small intestine.

Animals↗

PubMed1999

Simian virus 40 T antigen induces p53-independent apoptosis but does not suppress erbB2/neu gene expression in immortalized human epithelial cells.

Previously, we have shown that simian virus 40 (SV40) T antigen can directly cause apoptosis in immortalized human epithelial cells under normal growth conditions. In this study, we further characterized the mechanism of T-antigen-mediated apoptosis involving p53 and whether T antigen can suppress erbB2/neu gene expression. Our results show the differential regulation of erbB2/neu gene expression in different cell clones in response to T antigen transgene, indicating that the regression of erbB2/neu gene by SV40 T is cell-type dependent. Our previous study reported T-antigen-induced apoptosis in p53 mutant cells; however, in this study we find increased levels of p53 protein in T-antigen-containing cells. Therefore, we examined the transactivation function of p53 in these cells. Our data show the failure to transactivate p53, suggesting that increased p53 protein in T antigen expressing cells is functionless at least for transcriptional activation.

Antigens, Viral, Tumor↗

PubMed1999

Dobutamine-induced hypotension is an independent predictor for mortality in patients with left ventricular dysfunction following myocardial infarction.

Dobutamine echocardiography was performed on 297 patients after acute myocardial infarction to assess the prognostic value of dobutamine-induced hypotension in patients with left ventricular dysfunction. Patients were divided into two groups according to ejection fraction (group I, ejection fraction <0.45, n = 123; group II, ejection fraction > or =0.45, n = 174) and were followed for 20+/-8 months. Hypotension was defined as a decrease in systolic blood pressure > or =20 mm Hg, compared with baseline values. The incidence of hypotension was similar in groups I and II (23.6% vs. 18.4%, P = 0.28), and the hypotension was not related to positive dobutamine echocardiography. Univariate analysis showed that the development of hypotension was associated with a higher incidence of cardiac death in group I but not in group II. Multivariate analysis showed that dobutamine-induced hypotension was an independent predictor only for cardiac death in group I and was not related to any other cardiac events in either group. In conclusion, the development of hypotension during dobutamine stress can identify a subgroup with poor ventricular functional reserve and at high risk for cardiac death among patients complicated with left ventricular dysfunction.

Adrenergic beta-Agonists↗

PubMed1999

Primary central nervous system non-Hodgkin's lymphoma (PCNSL): survival advantages with combined initial therapy? A final report of the North Central Cancer Treatment Group (NCCTG) Study 86-72-52.

PURPOSE: We herein report updated survival and toxicity data on the entire cohort of 53 eligible patients treated on North Central Cancer Treatment Group (NCCTG) protocol 86-72-52, which is now closed. METHODS AND MATERIALS: An initial report was published in this journal in 1995. No substantive changes in the conclusions of that report were identified in this analysis. Median survival was 9.6 months for the entire cohort; median survival for the 20 patients who completed the prescribed protocol treatment was 20.7 months. The hematologic and non-hematologic toxicity distributions are virtually the same as those reported in the original paper. RESULTS: Results are given for the entire group and for subsets defined by age < or = 60 versus > 60 years, and < 70 versus > or = 70 years of age. CONCLUSIONS: No significant differences were observed in any of the outcome variables by age group. There was, however, a nonsignificant suggestion of poorer outcome in those who were > 60 years of age.

Adult↗

PubMed1999

Gender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin gene.

A sensitive technique for the sexing of bovine embryos was developed using polymerase chain reaction (PCR) amplification of the bovine amelogenin (bAML) gene on the X- and Y-chromosomes of Holstein dairy cattle. Cloning and DNA sequencing showed a 45.1% homology between the fifth intron of the bAML-X and bAML-Y gene with multiple deletions. A pair of sex-specific primers was designed to allow amplification of a single fragment of 467-bp from the X-chromosome of female cattle and two fragments of 467-bp and 341-bp from the X- and Y-chromosomes of male cattle. The primers were successfully applied to bovine sexing from single blastomeres isolated from day-6 to day-7 cow embryos by direct cell lysis and PCR. Our protocol of embryo sexing should be applicable to the diagnosis of defective genes in vitro in human embryos and in other domestic or recreational animals.

Amelogenin↗

PubMed1999

Transient apoptosis elicited by insulin in serum-starved glioma cells involves Fas/Fas-L and Bcl-2.

The expression of fas gene in glioma cells varies with growth stage. When insulin-elicited transient apoptosis of glioma cells was in progress, the expression of fas gene increased at both transcriptional and translational levels. In contrast, the expression of fas-L gene in glioma cells remained constant. Apoptosis occurred in the cells having high level of surface Fas protein. When the expression of Fas-L in U-373MG cells was suppressed by ribozyme, the insulin-elicited transient apoptosis vanished. Overexpression of Bcl-2 in U-373MG cells did not alter significantly the cell cycle progression and the expression of fas gene. However, these cells were resistant to insulin-trigged death. Therefore, insulin-elicited apoptosis involved Fas-related death signal, and which could be prevented by the protective effect of Bcl-2.

Antigens, Surface↗

PubMed1999

A new picorna-like virus, PnPV, isolated from ficus transparent wing moth, Perina nuda (Fabricius).

Two viruses, Perina nuda nucleopolyhedrovirus and a new picorna-like virus, were previously isolated from P. nuda larvae with flacherie. In this study the new picorna-like virus was characterized using physical and biochemical methods. This small virus appears to belong to the family Picornaviridae and we propose the name PnPV. PnPV can be propagated in its homogenous cell line, NTU-PN-HH. PnPV purified from the cell line resembles PnPV isolated from insects: under electron microscopy, it exhibits icosahedral symmetry, measures 30 nm in diameter, and has no envelope and no distinct surface structure in negatively stained preparations. In addition, we show here that PnPV has a buoyant density of 1.381 g/ml in cesium chloride, the viral genome was composed of one single-stranded RNA molecule with a length of 10 kb, and poly(A) tract and polyacrylamide gel electrophoresis of purified viral particles revealed three major (31.5, 29.7, and 28.4 kDa) and three minor (27. 0, 24.5, and 4.0 kDa) structural proteins.

Animals↗

PubMed1999

Stretching and strengthening exercises: their effect on three-dimensional scapular kinematics.

OBJECTIVE: To quantitatively evaluate the effects of commonly used shoulder exercises on shoulder kinematics and resting posture. STUDY DESIGN: A repeated-measures design was used with measurements performed before and after a 6-week exercise program. METHOD: Twenty asymptomatic subjects with forward shoulder posture were recruited. Stretching exercises for the pectoral muscles and resisted strengthening exercises for the scapular retractors and elevators and the glenohumeral abductors and external rotators were performed three times per week for 6 weeks. A three-dimensional electromechanical digitizer was used to measure thoracic inclination and scapular orientation and position. These measurements were taken with the arm (1) at the side, (2) abducted to 90 degrees, and (3) at maximal abduction. The isometric force of glenohumeral external and internal rotation and horizontal abduction and adduction were measured with a hand-held dynamometer. All subjects were tested before and after the 6-week exercise program. Hotelling's T2 and paired t tests were used for data analysis. RESULTS: The strength of horizontal abduction and internal and external rotation increased after exercise (p < .01). The anterior inclination of the thoracic spine decreased, and the glenohumeral contribution to arm elevation increased (p < .01). Resting scapular posture did not change. As the arm was abducted to 90 degrees, the scapula showed less upward rotation and less superior translation after the exercise program (p < .01). CONCLUSION: The exercise program improved muscle strength, produced a more erect upper trunk posture, increased scapular stability, and altered scapulohumeral rhythm.

Adult↗

PubMed1999

Anterior ankle-foot orthosis effects on postural stability in hemiplegic patients.

OBJECTIVES: To evaluate the effects of an anterior ankle-foot orthosis (AFO) on static and dynamic postural stability in hemiplegic patients. DESIGN: A cross-sectional assessment of hemiplegic subjects with and without an AFO. SETTING: Outpatient department of a rehabilitation hospital. PATIENTS: A convenience sample of 24 subjects who had been prescribed an anterior AFO. OUTCOME MEASURES: Postural sway index and postural symmetry (body weight distribution through the affected leg) when standing were measured as static postural stability. Maximal balance range in anterior-posterior and lateral directions and the affected leg's weight bearing after weight shift to affected side were measured as dynamic postural stability. RESULTS: When wearing the anterior AFO, there was no significant difference and small effect size (r<0.3) in postural sway index (p = .35), postural symmetry (p = .21), and maximal balance range in anterior-posterior direction (p = .46). There was a significant improvement and large effect size (r>0.5) in lateral weight shifting (p<.01) and weight bearing through the affected leg after weight shifted to the affected side (p<.01). CONCLUSIONS: The significant effects of the anterior AFO in long-term hemiplegic patients were on lateral weight shifting and weight bearing through affected leg after weight shifted to the affected side. Postural sway, postural symmetry, and anterior-posterior weight shifting were not significantly affected.

Adult↗

PubMed1999

Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: the role of nitric oxide.

BACKGROUND: Asthma exacerbation is associated with increased numbers of circulating CD34(+) progenitor cells, which may migrate to airways and develop into mature cells under the effects of cytokines and hematopoietic factors. Nitric oxide (NO) generation is enhanced in asthma and is known to suppress human hematopoiesis. OBJECTIVES: We studied circulating progenitor cells in the blood of patients with varying severity of asthma and examined the contribution of NO to their proliferation into eosinophil-forming colonies ex vivo. METHODS: With use of multiparameter flow cytometric analyses, the cell numbers and intracellular inducible NO synthase (iNOS) immunoreactivity of circulating CD34(+) cells in peripheral blood was measured. The serum level of GM-CSF or IL-5 was also determined. The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors, including GM-CSF, stem cell factor, and IL-3. RESULTS: A significantly greater number of circulating CD34(+) cells increased together with higher intracellular iNOS immunoreactivity in moderate asthmatics compared with mild intermittent asthmatics and healthy subjects. There was no significant difference in iNOS immunoreactivities or CD34(+) progenitor cell numbers between healthy subjects and those with mild intermittent asthma. Serum levels of GM-CSF or IL-5 were significantly higher in all asthmatics compared with healthy subjects and correlated with circulating CD34(+) cells. A greater number of colonies was grown either in the presence or absence of growth factors with a higher percentage of cells of eosinophil lineage in asthmatics than in health subjects. N(G)-nitro-L-arginine methyl ester potentiated and sodium nitroprusside inhibited the colony growth in both asthmatic and healthy subjects without a significant change in the percentage of eosinophil lineage. CONCLUSIONS: The production of NO from progenitor cells or other circulating cells may act in an autocrine or paracrine fashion to regulate progenitor cell growth and colony formation. However, this is not sufficient to control the increased proliferation of progenitor cells observed in asthma.

Adult↗

PubMed1999

Assessment of the function and effect of diabetes education programs in Taiwan.

A multi-center prospective study was conducted to assess the function and impact of diabetic education programs on diabetic control. A total of 208 subjects with type 2 diabetes were recruited. Diabetes self-care, assessed by questionnaire, was evaluated before, and 4 months after attending a diabetes education course. A total of 121 subjects who received advanced diabetes education courses were designated as the experimental group. A second group of 87 cases receiving a basic course served as controls. In addition to basic knowledge, the advanced education programs included dietary control, blood glucose monitoring, management of hypoglycemia, medication compliance, foot care and exercise. Diabetes self-care techniques were significantly improved in the experimental group. The overall score for diabetes self-care techniques improved in both groups at the 4th month over baseline values. The change was significant with the controls' (P < 0.001). Multiple regression analysis confirmed the intensity of diabetic education was the only significant variable correlated with the decrease of fasting blood glucose and systolic blood pressure. In conclusion, integrated and intensive diabetes education program in diabetes education centers provides an effective method for improving diabetes self-care techniques and metabolic outcome.

Aged↗

PubMed1999

Automatic finite element mesh generation for maxillary second premolar.

Developing three dimensional finite element mesh models for irregular geometric objects requires a large amount of manual efforts, hence limiting the three dimensional approach for dental structure analyses. An automatic procedure which can be used to generate a three dimensional finite element mesh for the maxillary second premolar was developed in this study. Firstly, a embedded second premolar was sliced and scanned parallel to the occlusal surface. A self-developed image processing system was employed to detect the boundaries of different materials within each section. An automatic mesh generation program was used on these boundaries to create tetrahedral elements based on moving nodes of uniform cube approach. Six mesh models of the second premolar with different element sizes using linear and quadratic elements were analyzed. Strain energy and von Mises stresses were reviewed for convergence in the crown regions.

Algorithms↗

PubMed1999

Controlled release of human immunoglobulin G. 1. Release kinetics studies.

The release of human immunoglobulin G (IgG) using ethylene-vinyl acetate copolymer (EVAc) as a polymer carrier was studied by fabricating them into two commercially available dosage forms: slab and microsphere. A first-order flux decay model and two hierarchical models concerned with the mass transfer coefficient on the slab surface were used to describe the mechanism of release kinetics and the results compared. These models gave insight to some of the important physical parameters of drug release such as the diffusion coefficient, time constant of release, and initial flux. It was found that the release mechanism varies with time, and hence no single model can be used to predict the release profile for the entire period of study. A controlled release study by matrix coating was also done. The results obtained were utilized to examine the suitability of a particular dosage form (matrix geometry) of IgG for clinical applications. The release data compared with the standard methods of IgG therapy proves localized drug delivery to be a major boon for immunodeficient patients.

Algorithms↗

PubMed1999

Controlled release of human immunoglobulin G. 2. Morphological characterization.

Human immunoglobulin G (IgG) serves as an important chemotherapeutic agent for a number of immunological ailments and as a carrier in the targeted delivery of other therapeutic agents. This paper deals with the characterization of IgG-dispersed monolithic matrixes of different geometries, prepared using a nonbiodegradable polymer carrier EVAc. The morphological changes associated with the matrix during drug release was studied using scanning electron microscopy, polarizing microscopy, atomic force microscopy, and X-ray photoelectron microscopy, and the results were compared. The study answered the burst effect problem significantly and illustrated the potential of these techniques in understanding the morphological structure of matrixes and mode of release kinetics.

Delayed-Action Preparations↗

PubMed1999

Monoclonal anti-androgen receptor antibodies: production, characterization and potential diagnostic applications.

Several monoclonal antibodies (mAbs) and novel mAb-based assays for the androgen receptors (AR) have been developed. Large amounts of the recombinant human AR protein produced by a baculovirus expression system were used as an antigen to produce mAbs. Twenty-nine AR-specific mAbs were first confirmed by Western blot analysis and were then characterized for their immunoglobulin isotypes, epitopes, and epitope localization in AR. Novel assays using flow cytometry and sandwich enzyme-linked immunosorbent assays (ELISA) were established to detect AR-expressing cells and to quantify soluble AR protein, respectively. Using immunostaining, we identified several anti-AR mAbs exclusively recognizing AR within the nuclei of the prostate cancer cell line LNCaP and of prostate tissues in both frozen and paraffin-embedded sections, whereas other mAbs could detect AR in both nuclear and cytoplasmic compartments. Interestingly, certain mAbs, such as G122-25 and G122-77, could distinguish the androgen-bound AR from the unoccupied AR. In sum, many purified AR protein and anti-AR mAbs, together with the assays developed, could be powerful tools for the study of functional AR and for the diagnosis of prostatic cancers.

Animals↗

PubMed1999

Extramedullary relapse after all-trans retinoic acid treatment in acute promyelocytic leukemia--the occurrence of retinoic acid syndrome is a risk factor.

All-trans retinoic acid (ATRA) is now a standard agent for remission induction of acute promyelocytic leukemia (APL). Recently, extramedullary relapse, which was a rare condition in APL patients after chemotherapy alone, was reported with an increased frequency after ATRA treatment. However, it is not yet clear whether ATRA truly increases the risk of extramedullary recurrence and what are the risk factors. In this study, three of 13 patients with recurrent APL after prior treatment of ATRA were found to have extramedullary involvement, compared with none in 11 recurrent patients previously treated with chemotherapy alone (estimated relative risk 2.100, 95% confidence interval 1.341-3.289). Furthermore, in the former group of patients, the development of retinoic acid (RA) syndrome during prior induction treatment was significantly associated with extramedullary involvement at relapse (three in five patients with RA syndrome vs none in eight without the syndrome, estimated relative risk 5.000, 95% confidence interval 1.448-17.271). In conclusion, ATRA may predispose APL patients to extramedullary involvement at relapse and the occurrence of RA syndrome is a risk factor for it. Further studies are needed to confirm these findings. It also remains to be clarified whether treatment modification is necessary in patients who develop RA syndrome during ATRA treatment.

Adolescent↗