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Biomedical subjects

C Hagengruber

Publications and source records attributed to C Hagengruber.

8 recordsLinked to original sources
PubMed1990

Characterization of common variable immunodeficiency: identification of a subset of patients with distinctive immunophenotypic and clinical features.

The peripheral blood lymphocyte surface markers and clinical features of 38 patients with common variable immunodeficiency (CVID) were assessed. These studies identified a subset of CVID consisting of 14 of the 38 patients with a distinctive T-cell immunophenotype and clinical findings. The phenotypic changes were characterized by an abnormally low CD4/CD8 ratio (less than or equal to 0.9) due primarily to a significant increase in CD8 T cells. In addition, there was an expansion in CD8 T cells coexpressing CD57 and increased expression of the activation markers HLA-DR and interleukin-2 receptor (IL-2R) by these cells. This group of immunophenotypically abnormal CVID patients also had characteristic clinical features, including splenomegaly (P less than .02) and in vivo T-cell dysfunction based on the evaluation of delayed-type hypersensitivity (P less than .05). Approximately 71% of these patients had splenomegaly and 42% were anergic in contrast to the remaining group of CVID patients, in which 29% had splenomegaly and 7% were anergic. These findings define a subgroup of CVID patients that have specific immunophenotypic features and functional T-cell abnormalities.

Adult↗
PubMed1988

Two-color flow cytometric analysis of monocyte depleted human blood lymphocyte subsets.

Peripheral blood mononuclear cells (PBMC) from normal individuals were examined using 16 pairs of FITC and phycoerythrin (PE) directly conjugated monoclonal antibodies. Each pair of reagents was used to evaluate a conventional lymphocyte gate as well as open (non) gate of monocyte depleted PBMC. Parallel studies using the same panel of monoclonal antibodies were carried out on selected, nonmonocyte depleted samples. The major findings of this analysis were that 1,000-1,200 lymphocytes in a 10,000 cell analysis are found outside the lymphocyte gate and of these approximately 2/5 are CD16 positive LGL/NK cells, 2/5 are CD3 positive T cells, and 1/5 are CD19/CD20 positive B cells. Thus, it appears that 10-15% of the lymphoid cells fall outside of the conventional lymphocyte gate, and in certain settings monocyte depletion may be useful to perform more complete evaluation of the total lymphoid cell population obtained after ficoll-hypaque separation.

Antibodies, Monoclonal↗
PubMed1986

Automated determination of urinary creatinine without sample dilution: theory and practice.

The rate of the Jaffé reaction depends on the concentration of sodium hydroxide; the pseudo-first-order rate constant of the reaction, at 37 degrees C in 10 mmol/L picrate solution, is 0.004 mmol/L. We formulated an automated method to determine urinary creatinine directly without manual sample dilution. The conditions are as follows: 10 mmol/L picrate and 60 mmol/L sodium hydroxide (final concentrations); ratio of sample to final volume, 1:41; temperature, 37 degrees C; wavelengths of measurement, 500 or 510 nm; interval of measurement, 30 to 90 s; and mode of measurement, kinetic. Determinations of creatinine in patients' samples by the new method compared favorably with those obtained with the AutoAnalyzer and aca. The run-to-run CVs were 3.6% or less, and the method was accurate for concentrations of creatinine up to 3000 mg/L. We recommend this method as a good replacement for the AutoAnalyzer or aca methods.

Autoanalysis↗
PubMed1985

Comparison of creatinine as determined with the Ames Seralyzer and by three Jaffé-based methods.

We compared results for urinary creatinine, serum creatinine, and creatinine clearance, as determined with the Ames Seralyzer, with results determined with the Beckman ASTRA, the DuPont aca, and Technicon's AutoAnalyzer and SMAC. Results for urinary creatinine from the Seralyzer differed significantly (p less than 0.05) from those obtained with the ASTRA and AutoAnalyzer, but not with the aca. The Seralyzer results for serum creatinine were at least 1.0 mg/L higher (p less than 0.05) than by the other three methods. Results for creatinine clearance from the Seralyzer were 8 to 11 mL/min lower (p less than 0.05) than results by the other three methods. These differences are related to the positive interference by bilirubin in the Seralyzer creatinine method. We also evaluated 23 other compounds for interference with these methods for creatinine.

Autoanalysis↗
PubMed1984

Reaction of picrate with creatinine and cepha antibiotics.

The concentration of creatinine in serum, which is used to estimate glomerular filtration rate, is measured by reaction with alkaline picrate, but this reaction is not specific for creatinine. Although several other cephalosporin antibiotics have been reported not to react with picrate, we reacted picrate with creatinine, cefoxitin, penicillin, and eight different cephalosporins, and found that all compounds reacted with picrate and showed superimposable spectrophotograms with absorption maxima at 485 nm. From these results we conclude that the color-absorbing moiety of the product is the picrate molecule. Further, the structure common to creatinine and the cephalosporins, cefoxitin, or penicillin is the carbonyl group attached to a nitrogen and a carbon atom. We postulate that the carbonyl group with the adjacent carbon and nitrogen atoms is probably the chemical moiety that reacts with picrate to absorb energy at 485 nm.

Cephalosporins↗
PubMed1984

Effect of dialysis on interference by cefoxitin with determination of creatinine.

Interference by cefoxitin with determination of creatinine is less with the Technicon SMAC than with other commercial analytical systems. The SMAC assay involves a single-point kinetic method with dialysis, whereas most other commercial methods are multipoint kinetic without dialysis. The apparent creatinine concentration measured for aqueous solutions of cefoxitin was 73 mmol of creatinine per mole of cefoxitin with the SMAC, 135 mmol/mol with a manual method. Furthermore, we determined for the SMAC that the average fraction of creatinine dialyzed was 0.128 and for cefoxitin, 0.064. Thus, the concentration of and interference by cefoxitin in the reaction mixture for SMAC are reduced by half (i.e., the apparent creatinine concentration for cefoxitin with the manual system multiplied by 0.5 is essentially that noted with the SMAC: 68 vs 73). Thus we conclude that the diminished interference be cefoxitin with determination of creatinine by SMAC is primarily ascribable to the dialysis step.

Autoanalysis↗