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Biomedical subjects

C Hecht

Publications and source records attributed to C Hecht.

7 recordsLinked to original sources
PubMed2005

Liquid chromatographic-mass spectrometric-analyses of anaerobe protein degradation products.

A liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) method and a liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry (LC-APCI-MS) method was developed to identify metabolites from the anaerobe protein degradation to biogas. As consequence of a process failure the biogas production breaks down with increasing substrate loading, whereas different metabolites accumulate in the fermentation media. These compounds were identified as metabolites from the anaerobe degradation of the aromatic amino acids phenylalanine, tyrosine and tryptophan and accumulate in concentrations up to 300 mg/L when casein was used as model substrate.

Anaerobiosis↗
PubMed1996

Variants within the 5'-flanking region and the intron I of the bovine growth hormone gene.

In order to identify DNA variants potentially involved in the regulation of the expression of the bovine growth hormone gene, the 5'-flanking, the 5'-untranslated and the signal peptide coding regions of 15 cows (four German Friesian, two German Simmental, two Jersey, two Galloway, two Scottish Highland, two Charolais and one Ceylon Dwarf Zebu) were analysed. For each animal a region of about 1.2 kb was amplified by polymerase chain reaction (PCR) and sequenced. Six sites of variable nucleotides were identified in the 5'-flanking region of the gene and one in the intron I. Some of these variable sites are also potential binding sites for trans-acting factors (CAAT/enhancer binding protein (C/EBP), polyoma virus enhancer A binding protein 3 (PEA3), thyroid hormone response element (TRE)) and therefore possibly involved in the expression of the growth hormone gene. PCR amplification of specific alleles (PASA) tests for two of the sites (G2, G3) were established and the genotypes for these variants were analysed in 190 cows. The frequencies of the C alleles of the sites G2 and G3 were 0.63 and 0.76, respectively.

Animals↗
PubMed1992

Surface sensibility of the floor of the mouth and tongue in healthy controls and in radiated patients.

Increased areas of anesthesia in the oral cavity have been shown to significantly impair oral function in normal individuals. In patients who undergo oral cavity reconstruction, loss of sensation plays a major role in producing disturbances in postoperative oral function. Free tissue transfer techniques have permitted the problem of sensory loss to be addressed through the use of sensate cutaneous free flaps, in which microneural anastomoses are performed between a sensory nerve supplying the flap and a recipient nerve in the head and neck. To critically assess the results of such reconstructions, the effect of sensory restoration on oral cavity rehabilitation must be studied. As a first step toward this goal, normal values for sensory discrimination of the floor of mouth and tongue are needed. Previous studies of oral sensation failed to examine the ventral tongue and floor of mouth. The purpose of this study is to determine the surface sensibility of these regions in healthy patients and in patients who received radiation therapy to the oral cavity. Sensation was evaluated using static and moving two-point discrimination in 90 healthy subjects divided equally into three age groups: 20 to 40 years, 41 to 60 years, and 61 to 80 years. In addition, 20 patients who received radiation therapy were studied. The mucosa of the dorsal and ventral aspects of the lateral tongue, tongue tip, and floor of mouth was examined. The tongue tip is the most sensitive area, followed by the dorsal lateral tongue, ventral lateral tongue, and floor of mouth. The effects of age and radiation therapy on sensory discrimination are discussed.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1979

Surgical decision-making in the treatment of pancreatic pseudocysts. Internal versus external drainage.

One hundred patients with documented pancreatic pseudocysts who underwent surgical drainage are reported on. Accurate assessment of the size and location of the pseudocyst using ultrasonography, endoscopic retrograde cholangiopancreatography, angiography, and upper gastrointestinal roentgenography is essential. After diagnosis, the next 4 to 7 weeks is a critical period, after which surgical intervention becomes mandatory if the cyst has not resolved. This period between diagnosis and operation is hazardous and demands close clinical follow-up. When oepration is required, the preferred procedure continues to be internal drainage because it is associated with less morbidity and a lower mortality. However, external drainage appears to be a suitable second choice. Fewer than 10 per cent of the patients in this series who underwent external drainage developed fistulas, and the overall rate of late morbidity was acceptable.

Abscess↗
PubMed1978

Villous adenomas of the duodenum.

A patient with multiple villous adenomas of the duodenum is described. Endoscopy plays an essential role in the management of these neoplasms. If no evidence of invasive malignancy is found on multiple endoscopic biopsies, wide local excision is the initial procedure of choice. Invasive malignancy found in either the endoscopic biopsy or the surgical specimen is indication for pancreaticoduodenectomy.

Adenoma↗
PubMedDate not supplied

Association of generalized joint hypermobility with history, signs, and symptoms of temporomandibular joint dysfunction in children.

This study determined the prevalence of signs and symptoms of temporomandibular joint dysfunction (TMD) in children with and without generalized joint hypermobility (GJH). Twenty children with GJH, ages 4-19, and 20 age- and sex-matched control children completed a TMD signs/symptoms history and underwent an examination consisting of palpation of the joints and associated musculature for tenderness, clicking, or crepitation. Children with historical or clinical findings were designated positive for TMD signs/symptoms. Maximum vertical opening, expressed as a percentage of lower facial height, and maximum mandibular lateral excursion (in mm) were recorded. Fifteen (75%) of the GJH participants and ten (50%) of the controls were positive for TMD findings. There were statistically significant differences between the groups for the presence of total positive findings (P < 0.001) and for responses to palpation of muscle or joint (P = 0.03). There were no significant differences in positive responses to the history alone, joint palpation alone, or muscle palpation alone. There were no significant differences between the groups in jaw excursions. This study suggests that children with GJH may be more likely to demonstrate some signs and symptoms of TMD than children with normal joint mobility.

Adolescent↗