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PubMed1984

[Low-dose cytosine arabinoside (ARA-C) in the treatment of acute myeloid leukemia].

Eleven patients over 70 years of age and two patients under 60 years of age with myeloblastic leukemia have been treated with low doses of cytarabine (10 mg per m2 every 12 hours by subcutaneous injection for a median duration of 3 weeks). Four patients of the first group went into complete remission, 2 patients in the older age group and the 2 patients under 60 years of age went into partial remission, while only 3 patients out of 13 (23%) did not respond to this treatment. Sometimes severe cytopenia observed during the induction phase counterbalanced the advantages of treatment (simplicity and absence of systemic toxicity) and suggest an antimitotic effect which is probably associated with differentiation of the malignant clone.

Adult↗

PubMedDate not supplied

T-cell repertoire complexity after allogeneic bone marrow transplantation.

We analyzed the T-cell repertoire in patients transplanted with bone marrow from an HLA identical sibling by determining the TCR diversity through Vbeta-CDR3-size spectratyping with Vbeta/Cbeta- and Vbeta/Jbeta-specific primers. Using the Vbeta/Cbeta primers, we observed limited TCR diversity only in recipients of a T-cell-depleted graft, whereas the TCR diversity of patients transplanted with an unmanipulated graft seemed to be indistinguishable from the one of a normal individual. However, with Vbeta/Jbeta-specific primers, increase of the resolution by approximately 10-fold also allowed the detection of imbalances in the TCR repertoire of recipients of an unmanipulated graft. This demonstrates that when high numbers of T cells are cotransfused with marrow, the TCR repertoire is more complete but still not as complete as in normal individuals, thereby emphasizing the important role of coinfused mature T cells in the restoration of the T-cell compartment after bone marrow transplantation.

Base Sequence↗