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Biomedical subjects

C Henning

Publications and source records attributed to C Henning.

At least 37 records · Page 2Linked to original sources
PubMed1984

Static and dynamic properties of tissue cage fluid in rabbits.

The properties of tissue cage fluid in a steel net tissue cage model in rabbits were compared to those of serum by determination of the protein profile, the cell contents, and the pharmacokinetics of 125I albumin, 3H sucrose and 3H fusidic acid. The dominating serum proteins demonstrated by crossed immunoelectrophoresis were also detected in tissue cage fluid but at lower levels and at various ratios. The cell pattern gradually changed from an initial dominance of polymorphonuclear cells to lymphocytes during the five weeks following the subcutaneous implantation of the cages. The distribution of the highly protein-bound fusidic acid was markedly slower and the maximal tissue cage fluid level significantly lower than that of sucrose. Equilibrium of 125I albumin between serum and tissue cage fluid was slowly achieved during the following two weeks. The advantages and disadvantages of tissue cage models for studies of drug pharmacokinetics are discussed. The properties of tissue cage fluid and the possibility of repeated sampling make the model suitable for studies of experimental local infections. The influence of therapeutic agents and the host's response to the infectious process may also be elucidated.

Albumins↗
PubMed1984

Comparative study of netilmicin/tinidazole versus netilmicin/clindamycin in the treatment of severe abdominal infections.

The efficacy of netilmicin combined with tinidazole (N + T) or clindamycin (N + C) in the treatment of severe abdominal infections was evaluated in a prospective randomized study with 20 patients in the N + T group and 21 patients in the N + C group. Normally the maintaining dose for netilmicin was 2.25 mg/kg every 12 h, for tinidazole 400 mg every 12 h and for clindamycin 300-600 mg every 6-8 h. The mean duration time of treatment was 8 days in the N + T group and 10 days in the N + C group respectively. In the N + T group 18 patients were cured and in the N + C group 17 patients. Among aerobic bacteria Escherichia coli was most frequently isolated and among anaerobes Bacteroides sp. All aerobic bacteria with 2 exceptions were susceptible to netilmicin and all anaerobic bacteria but 2 to tinidazole or clindamycin. Adequate serum levels were obtained for each antibiotic during therapy. In this study with a small number of patients the combination of netilmicin and tinidazole was as effective as netilmicin and clindamycin.

Adolescent↗
PubMed1983

Pivmecillinam in treatment of Staphylococcus saprophyticus urinary tract infections.

In a non-randomised trial 15 women with Staphylococcus saprophyticus bacteriuria were treated with pivmecillinam 200 mg t.i.d. for 1 week. At follow-up 2-4 weeks post treatment only 11 patients were cured. As a complement to the study the occurrence of Staph. saprophyticus bacteriuria was analysed according to age, sex and season. Mecillinam MIC's and viability curves of Staph. saprophyticus were investigated. It is concluded that mecillinam should only be used to treat urinary tract infections caused by bacteria sensitive in vitro.

Age Factors↗
PubMed1982

Serum assays of netilmicin by enzyme multiplied immunoassay technique and bioassay: a comparative study.

Serum concentration determinations of netilmicin obtained with bioassay and the enzyme multiplied immunoassay technique were compared. There was no statistically significant difference between the precision of the two techniques (P greater than 0.05). When clinical serum was used, a systematic error was demonstrated between the enzyme multiplied immunoassay technique and bioassay.

Biological Assay↗
PubMed1982

Penetration of alafosfalin and ampicillin into tissue cage fluid in rabbits.

The pharmacokinetics of alafosfalin and ampicillin were studied in rabbits. Serum concentrations were compared to levels in subcutaneous tissue fluid obtained from implanted tissue cages. The concentrations of alafosfalin exceeded those of ampicillin and the drugs were eliminated at similar rates from serum and tissue fluid.

Alanine↗
PubMed1982

Rapid technique for detection of pneumococcal pneumonia.

Tracheal aspirates from patients with diarrhoea and pneumonia were tested for the presence of pneumococci by both quantitative culture and counter immunoelectrophoresis (CIE). Antigen was demonstrated by CIE in 33/48 of these confirmed cases of pneumonia. The technique is easy to use and gives rapid results.

Adolescent↗
PubMed1981

Penetration of ampicillin and dicloxacillin into tissue cage fluid in rabbits: relation to serum and tissue protein binding.

The pharmacokinetics of ampicillin and dicloxacillin following intravenous injection was studied in a tissue cage model in rabbits. Serum protein binding was 98.6% for dicloxacillin and 46.7% for ampicillin, and there were no major differences between the degree of binding in tissue case fluid. The highly bound dicloxacillin gave lower but more sustained levels in tissue cage fluid than ampicillin, and showed a very long elimination half-life. The findings suggest that the flow of albumin into the interstitial space could be of importance for the tissue penetration of highly protein bound antibiotics. The results also indicate dose-dependent pharmacokinetics for dicloxacillin.

Ampicillin↗
PubMed1981

Penetration of 14C-Ro 13-9904 into tissue cage fluid in rabbits.

The pharmacokinetics of Ro 13-9904/001 following i.v. injection of 40 mg/kg was studied in a tissue cage model in rabbits. Binding to rabbit serum proteins was determined by ultrafiltration and found to be 98% at a concentration of 100 microgram/ml. In the early post-injection period concentrations of the drug in rabbit serum reached 500-900 microgram/ml, the antibiotic binding capacity of serum proteins was exceeded and the outflow of drug into the tissues was high. Tissue cage fluid (TCF) concentrations increased during 4h, such as to reach a maximum level of about 30 microgram/ml. After 8 h a t1/2 of 15 h was calculated both in serum and TCF. From 36 h onwards the t1/2 was 75 h. After a single injection of 14C-Ro 13-9904/001 radiological activity could be followed in serum and TCF for 7-10 days and in urine for more than 2 weeks. The results are discussed and compared to those from similar experiments with benzylpenicillin and flucloxacillin.

Animals↗
PubMed1979

Comparison of Staphylococcus aureus carriage and skin infection rates in hospital and office employees.

The incidence of Staphylococcus aureus in the nose, throat and superficial wound infections of 99 office staff, 129 psychiatry staff and 115 surgical staff was studied over a 4-week period with the purpose of assessing the potential risk to hospital personnel of staphylococcal infection. Incidence rates, both average and cumulative, were essentially similar in the three groups but certain differences in the ecology of the staphylococcal phage groups were observed. Surgical staff appeared to have a more labile pattern of carriage. As in other Scandinavian studies throat carriage rates were high. Staphylococcal carriage seems largely to depend on individual characteristics rather than environmental factors.

Adult↗
PubMed1975

Bacterial contamination of urine, collected in fractions from different phases of micturition. A study in healthy women.

With a special technique 4-13 consecutive fractions of urine were collected during micturition from 4 female volunteers at together 12 occasions. Quantitative bacterial cultures were performed in 78 urine fractions. Most fractions contained one or several different bacterial strains-generally in small amounts, wheras only 14% were sterile. Fractions from the end of micturition showed on average less bacterial contamination than the earlier portions. No difference was observed between the bacterial counts after cleaning the area around the urethra as compared with the counts without preparation before sampling.

Adult↗