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Biomedical subjects

C Hertenstein

Publications and source records attributed to C Hertenstein.

14 recordsLinked to original sources
PubMed1983

Results with a modified human myeloma stem cell assay.

The human tumor stem cell assay (HTSCA) introduced by Hamburger and Salmon [2] was modified in several details. It was found that a short period of treatment with deoxyribonuclease (DNase) of the material aspirated from the patients bone marrow greatly enhances the production of single cell suspensions and thereby may improve the assay. Instead of the dextran sedimentation method, we used the density gradient centrifugation method according to Bøyum [1] for the isolation of mononuclear cells from the bone marrow. Another methodical modification introduced by us is the use of the same medium for culturing the cells before plating them and in the agar after plating. Under the conditions reported here the formation of colonies was observed in 8 of 11 samples from individual myeloma patients. The average plating efficiency was 0.024% with a range of 0.009% to 0.039% showing that possibly an improvement was achieved when compared to the results obtained with the original method showing an average plating efficiency of 0.014% [2].

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PubMed1982

Extramedullary multiple myeloma. A qualitative and quantitative electron microscopic study.

A case of multiple myeloma with a mediastinal tumor and other unusual findings is described. The findings included the presence of extramedullary tumor masses at multiple sites in addition to the medullary tumor and, more interestingly, the occurrence of pleural and peritoneal effusions. The plasma cells from these effusions were analyzed using stereological morphometric methods. The plasma cells were also cultured and established as a continuous cell line and were studied using the same methods. Our results indicate that there are qualitative and quantitative morphologic differences between the pleural and peritoneal plasma cells. Comparison between plasma cells and cultured cells from the intraperitoneal exudate showed marked morphologic differences. The analysis of these differences indicated that mature plasma cells, when subjected to culture, were transformed into immature lymphoid cells.

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PubMed1980

[In vitro studies on two cell lines from patients with Hodgkin's disease (author's transl)].

Two cell lines (Ma,Bi) were established in vitro from pleura effusions from two patients with Hodgkin's disease. After some months the monolayer line Ma spontaneously became nonadherent and proliferated in suspension. Immunological properties, enzyme activities, surface characteristics and chromosomal analyses suggested that the culture Ma is composed of lymphoid cells and macrophages. Line Bi propagated attached to the glass and had properties typical for macrophages only.

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