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Biomedical subjects

C Hipler

Publications and source records attributed to C Hipler.

At least 37 records · Page 2Linked to original sources
PubMed1989

[X-ray irradiation of the human epidermis in vitro. II. Comparison of a single 44 kV and 200 kV x-ray irradiation].

On example of the reduction of epidermal binding of FITC-wheat germ agglutinin (WGA) the direct membrane effect of a single roentgen irradiation (44 kV and 220 kV) was analysed in vitro. Human normal skin and psoriasis centre were compared. Normal skin showed no alteration of light-microscopically visible FITC-WGA-binding on epidermal cells over the whole doses range. Psoriatic lesions responded to doses of greater than or equal to 5 Gy (44 and 220 kV) with a drastic reduction of epidermal lectin adhesion to lower and middle cell layers. The maximum of efficacy was with 5 Gy (44 kV) or 10 Gy (220 kV). A dose elevation up to 20 Gy did not result in an increase of efficacy. Topographically the radiosensitive FITC-WGA-binding could be seen in the rete ridges above all. The findings support the impression of an increased radiosensitivity of the lesional psoriasis epidermis compared with normal skin. The causes for this are seen in an abnormal differentiation of keratinocytes in psoriasis.

Epidermis↗
PubMed1988

[Lectin histochemical study of epidermal nevi].

3 epidermal nevi were investigated by means of FITC-labeled lectins (LCA, RCA II, ConA, WGA). In comparison with normal skin, we observed selective modifications of the lectin-binding patterns. These findings suggest an increased presence of GlcNAc residues.

Adult↗
PubMed1988

Alterations of epidermal lectin binding sites in acute contact dermatitis.

We investigated alterations of epidermal lectin binding sites, as well as of pemphigus and bullous pemphigoid antigens, in 28 human patch test reactions, both allergic (nickel, formaldehyde, N,N'-1,3-dimethylbutyl-N'-phenylenediamine) and irritant (sodium lauryl sulfate). The epidermal reactivity to a panel of lectins and human antisera to pemphigus vulgaris and bullous pemphigoid antigens was compared with samples obtained from normal skin and from skin under tape occlusion. We observed selective perturbations of lectin and antibody binding in acute contact dermatitis, whether allergic or irritant. The main findings were a loss of terminal sialic acids and longer bi- and triantennary mannosyl residues as well as a loss of pemphigus vulgaris antigen. The only difference between allergic and irritant patch test reactions was in topography of loss of WGA binding sites: in the former, it was most pronounced in the lower and middle epidermis, whereas in the latter it was seen in the uppermost subcorneal layers. Our findings support a common pathway of cell membrane alterations of keratinocytes in acute contact dermatitis.

Adolescent↗
PubMed1988

[UV-A exposure of the human epidermis enhances the binding of antibodies to SSA/Ro in vitro].

Normal and psoriatic skin specimens (n = 15) were processed to give 4-micron-thick frozen sections and exposed to a single UV irradiation in vitro. We used monochromatic UV-B (313 nm) and UV-A (365 nm). The doses were as follows: 10(3)/10(4) J/m2 (UV-B) or 4.10(3)/8.10(4) J/m2 (UV-A). We investigated the effect on epidermal binding of antibodies to SSA/Ro using the indirect immunofluorescence technique. Untreated and UV-B-treated human skin failed to bind anti-SSA/Ro. UV-A exposure disclosed reticular or granular staining of epidermal nuclei and a perinuclear halo. The effect was nearly the same throughout the dosage range. The combination of UV-A and methoxsalen increased the intensity of staining. Normal and psoriatic skin behaved in the same way.

Autoantibodies↗
PubMed1987

[PUVA irradiation decreases the binding of membrane and basement membrane markers to human epidermis in vitro].

PUVA effects on frozen sections of human epidermis were investigated (20 micrograms 8-methoxypsoralen/ml; UVA wavelength 365 +/- 5 nm; radiation dose between 2 X 10(2) and 2 X 10(2) J/m2). The quality of the glycocalyx was characterized by lectins labeled with FITC (HPA, PHA, LCA, PNA, SBA, RCA, GCA I, UEA I). Serum antibodies of bullous pemphigoid (BP) served as membrane markers for the basement membrane. Fixed BP antibodies were detected by FITC-antihuman IgG. The fluorescence intensity was markedly decreased at a dose of 2 X 10(5) J/m2; 2 X 10(4) J/m2 were less effective. At 2 X 10(3) J/m2, there was nearly no alteration of the fluorescence intensity observed. Changes were seen in the case of BP antibodies and lectins, except UEA I. The relatively high intensity (radiation dose) and the effects immediately observable suggest direct membrane alterations by PUVA in vitro.

Antibodies↗
PubMed1987

[Effect of dithranol therapy on membrane, basement membrane and nuclear markers in psoriasis lesions].

We investigated the effects of anti-psoriatic therapy with dithranol (1/20-1%) in salicylic acid (0.5%) in white petrolatum on lesional skin. FITC-labeled lectins and pemphigus vulgaris antibodies (PV) served as analytical means to study the glycocalyx. Antibodies of bullous pemphigoid (BP) were used as basal membrane markers. Nuclear antigens were recorded according to the binding of speckled, anti-nuclear antibodies (ANA) as well as antibodies to dsDNA. With some lectins, dithranol therapy resulted in pronounced fluorescence of the lower parts of the basal cells. ConA was fixed by the basal cell layer. To a lesser degree, ANA were fixed by nuclei of keratinocytes. PV antibodies were not fixed at all.

Adult↗
PubMed1987

[Characterization of lectin binding by psoriatic epidermis--dependence on pH].

The binding of five different lectins by normal as well as psoriatic lesional epidermis was investigated with regard to its dependence on the pH value. As we studied the reactions within the pH range of 3.0 to 9.66, we obtained the following results: lectin binding almost independent from pH (FITC-Con A, -PHA, HPA), fluorescence intensity dependent on pH (TRITC-Con A, FITC-LCA, -GCA), binding pattern dependent on pH (FITC-LCA, GCA). In contrast to normal epidermis, there was no pH dependence of the FITC-GCA binding pattern in psoriatic lesional epidermis.

Humans↗
PubMed1986

[Effect of neutral solvents on lectin binding of healthy and psoriatic epidermis].

We studied the influence of neutral solvents on epidermal lectin binding in normal and lesional psoriatic skin. 1% solution of Tween 80 or Triton X-100 had no effect on the binding of the following lectins: PHA, ConA, and LCA. The HPA staining of the str. spinosum in normal skin, however, was completely removed by neutral solvents. In psoriatic skin, str. spinosum staining with HPA was resistant. Our results are discussed in the light of an altered keratinocyte maturation in psoriasis.

Humans↗
PubMed1986

[Lectin binding of psoriatic skin].

The aim of our study was the characterization of epidermal lectin binding pattern in psoriatic vs. non-psoriatic skin in order to reveal possible alterations of the glycocalyx composition of psoriatic keratinocytes. We used fluoroisothiocyanate-labeled lectins of Canavalia ensiformis (ConA), Phaseolus vulgaris (PHA), Lens culinaris (LCA), and Helix pomatia (HPA). The binding pattern of psoriatic (involved and non-lesional) skin did not differ from the control samples in ConA, PHA, and LCA. In psoriasis, there was a prominent HPA binding to the dermo-epidermal junction and to a lesser degree, intercellular epidermal near the uppermost cell layers. In seborrheic keratosis, in contrast, there was no fluorescence of the dermo-epidermal junction or the first layers of the epidermis. The most pronounced binding was observed perinuclear in the upper epidermis. The results are discussed in the light of an altered keratinocyte maturation in psoriasis.

Humans↗