PubMed Health⌕ Search

Biomedical subjects

C Hiramine

Publications and source records attributed to C Hiramine.

52 records · Page 3Linked to original sources
PubMed1977

Dissociation of blastogenic factor production and lymphocyte transformation of guinea pig lymphocytes stimulated with tuberculin PPD.

In vitro lymphocyte transformation of guinea pig lymph node cells induced by tuberculin PPD was essentially eliminated by treatment with 5-bromo-2-deoxyuridine and light. The remaining cell population was still capable of producing blastogenic factor and further, the amount of blastogenic factor released was comparable to that from the intact population in the same interval of culture. This result favors the conception that cells producing blastogenic factor in response to antigen are distinct from the cells responding to the antigen by DNA synthesis and proliferation.

Bromodeoxyuridine↗
PubMed1976

Dissociation of MIF activity and in vitro lymphocyte transformation in the development of experimental allergic thyroiditis in guinea pigs.

Macrophage migration inhibitory factor (MIF) activity and in vitro lymphocyte transformation to homologous thyroid antigen were compared in cultures of lymph node cells obtained from 123 guinea pigs at weekly intervals after a single injection of homologous thyroid extracts in complete Freund's adjuvant (CFA). MIF activity was not detectable at the early stage of immunization but became manifest 2 and 3 weeks after sensitization and a high level was maintained during 4-7 weeks. On the other hand, lymphocyte transformation preceded the appearance of MIF activity and attained its maximum 2 weeks after sensitization before the establishment of thyroiditis. It is indicated that MIF activity and lymphocyte transformation predominate separately in the course of immunization. There was a considerable correlation between intensity of MIF activity and severity of thyroiditis in individual animals during the advanced stage (4-6 weeks after sensitization) of the disease. Also, a lower but significant correlation was seen between lymphocyte transformation and severity of thyroiditis. However, about half of animals in the advanced stage of thyroiditis failed to exhibit antigen-stimulated lymphocyte transformation, despite a positive MIF activity.

Animals↗
PubMed1976

Effects of inhibitors of DNA and protein syntheses on mitogenic factor production by PPD-stimulated guinea pig lymphocytes.

The effects of inhibitors of DNA synthesis and mitosis and of protein synthesis on mitogenic factor production by lymph node cells from guinea pigs sensitized to tuberculin was studied. All inhibitors used in this study were added to the medium at the beginning of culture. Cytosine arabinoside, vinblastine and mitomycin-C reduced markedly the incorporation of tritiated thymidine in DNA, but had no effect on the production of mitogenic factor. Mitomycin-C at higher concentrations, however, induced the partial reduction of mitogenic factor production. On the other hand, puromycin and cycloheximide, inhibitors of protein synthesis, suppressed markedly the production of mitogenic factor. These results indicate that mitogenic factor production requires protein synthesis, but is independent of DNA synthesis.

Animals↗
PubMed1975

In vitro lymphocyte transformation in the development of experimental allergic thyroiditis.

In vitro lymphocyte transformation to homologous thyroid antigen has been studied in cultures of lymph node cells obtained from 97 guinea pigs at weekly intervals after a single injection of homologous thyroid extract in complete Freund's adjuvant, using a method for measurement of 3-H-thymidine incorporation. Lymph node cell cultures from animals 1 week after sensitization showed a significant increase in thymidine incorporation after the addition of antigen. Two weeks after sensitization the stimulation was maximal, and then a lower but significant response continued during the next 5 weeks. There was no correlation between lymphocyte transformation and the level of circulating antibodies throughout the post-sensitization period examined. The early lymphocyte transformation contrasted with the later development of macrophage migration inhibitory factor activity.

Animals↗
PubMedDate not supplied

New experimental model for adoptive transfer of murine autoimmune orchitis.

Previous studies demonstrated that experimental autoimmune orchitis (EAO) was produced in C3H/He mice with very high incidence by subcutaneous (s.c.) injections of viable syngeneic testicular germ cells (TC), without resorting to any adjuvants or immunopotentiators. Using this EAO model, a new and simple protocol was developed for adoptive transfer of EAO. Cell donors were C3H/He mice that received s.c. injections twice with TC alone. Spleen cells from the donors were stimulated in vitro with TC, propagated in interleukin-2 containing medium, then injected i.p. to naive recipient mice. This procedure induced severe orchitis and hypospermatogenesis with or without inflammation in epididymis and vas deferens in the recipients at high incidence. Elimination of all T cells or CD4+ T cells before the transfer produced no histopathological signs in the recipients whereas that of the CD8+ T cells or B cells had no inhibitory effect on the disease transfer, indicating that the effector cells are CD4+ T cells.

Animals↗
PubMedDate not supplied

Antigen non-specific tissue damage in T cell-mediated experimental autoimmune orchitis: preliminary characterization of a testis-specific T-cell line by using dermal tissue and cells.

A previous study demonstrated the establishment of a murine testicular antigen (mTA)-specific CD4+ T-cell line (designated BT.1) which was capable of transferring experimental autoimmune epididymo-orchitis to naive recipient mice. The disease transfer was antigen-specific, because no inflammatory lesion was observed in any other organs and tissues of the recipients. In this study, to investigate the local environment of BT.1 cells, the effect of the cells and their culture supernatant on a local tissue integrity was studied. When BT.1 cells were seeded on cultured fibroblastoid cell monolayers, the cells completely disrupted these monolayers in spite of the absence of the specific antigens. Moreover, the culture supernatant of BT.1 cells induced non-specific dermal inflammation when injected into skin tissue of normal syngeneic mice. Therefore, BT.1 cells were shown to devastate a tissue integrity and cause attraction and activation of inflammatory cells of the recipient origin in a local environment. These results suggest that the transferred BT.1 cells will specifically home to the testis and epididymis of recipients but the following devastation of seminiferous tubules and epididymal ducts might be non-specifically produced by the inflammatory cells of both donor and recipient origin in the lesion.

Animals↗
PubMedDate not supplied

Requirement of B lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) by lymph node cells.

The necessity of T lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) is an established fact. The present study was undertaken to elucidate the participation of B lymphocytes in the local transfer system of EAO. The capacity of purified lymph node T and B cells to transfer EAO was compared. Donor strain 13 guinea pigs were sensitized with testicular antigen and complete Freund's adjuvant. We then injected 1 X 10(8) cells just under the tunica albuginea of the testis of a syngeneic recipient. The recipients were killed 5 days after cell transfer. Nylon-wool nonadherent T cells alone produced only minimal lesions, but could induce more severe mononuclear lesions with the addition of syngeneic, normal macrophages. In contrast, B-cell enriched populations either alone or combined with a small number of macrophages, were able to transfer extensive lesions. In the combined transfer of immune T and immune B cells the severity of transferred lesions was dependent upon the number of B cells. These results suggest that cooperative interactions among T cells, B cells, and macrophages are involved in the generation of transferred testicular lesions, with emphasis on the participation of B cells.

Animals↗
PubMedDate not supplied

Effect of chemotherapy on Tl-201 tumor uptake: experimental study.

The purpose of this study was to investigate the correlation between histopathological changes and 201Tl accumulation in tumor following chemotherapy in the evaluation of chemotherapeutic effect. Walker 256 carcinoma implanted in the right thighs of Wistar rats was treated by the intravenous injection of adriamycin (ADR). 201Tl scintigraphy was performed before and 12, 24, 72, and 96 hours after chemotherapy. The count ratio of tumor to normal muscle (T/N ratio) was measured by gamma camera. Tumor growth rate was measured and histopathological examination of the tumor after 201Tl scintigraphy was performed. The tumors of all rats disappeared within 2 weeks after chemotherapy, but the T/N ratio did not change. Histopathological findings showed a decrement of tumor cells, small necrotic regions, and remarkable lymphocyte infiltration in the tumor bed in the healing stage. After 201Tl injection, lymphocytes isolated from tumor tissue showed noted 201Tl accumulation. These results suggest that the T/N ratio did not decrease after ADR chemotherapy because of the accumulation of 201Tl in lymphocytes. We concluded that the use of 201Tl may underestimate the therapeutic effect of ADR.

Animals↗