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C Hol

Publications and source records attributed to C Hol.

20 records · Page 2Linked to original sources
PubMed1989

Relation between splenic antibody formation and serum antibody levels to sheep erythrocytes as studied at the level of individual mice.

The relation between splenic antibody formation and serum antibody levels after intraperitoneal immunization of mice with sheep erythrocytes was studied in individual animals. A strong correlation between plaque-forming cell numbers in the spleen and haemolysin and haemagglutinin titres in the serum was observed. These results are at variance with recent data on the development of splenic auto-haemolysin-forming cells and corresponding serum levels after injection of mice with Escherichia coli endotoxin. The background behind the discrepancy between the antigen-driven immune response and the more polyclonal stimulation of B cells with endotoxin is being discussed.

Animals↗
PubMed1977

Centrifugation techniques and reagent strips in the assessment of microhaematuria.

During a survey for bladder cancer in a healthy male population a surprisingly high incidence of significant microhaematuria was found and no urinary sample was completely free of blood. A study of the sensitivity of the different techniques for assessing microhaematuria seemed necessary. A semiquantitative centrifugation technique, developed for cytological purposes, was found to be the most sensitive. Blood could be detected in concentrations of less than 50 RBC/ml urine. With conventional analysis, performed carefully, the minimum detectable concentration was about 500 RBC/ml. Reagent strips, based on the orthotholidin peroxidase reaction and developed specifically for urine analysis, gave positive reactions in minimum concentrations of 1 000 000 RBC/ml (original Hemastix), 50 000 RBC/ml (new Hemastix), and 20 000 RBC/ml (Sangur-Test). Positive scores with lower concentrations could be obtained with the Sangur-Test strip when it was read with a magnifying glass. However, the degree of erythrocyturia that may be regarded as physiological and that which is pathological has yet to be defined.

Aged↗