[Prospect of modern understanding and research of eight principal syndromes].
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Animals↗
Biomedical subjects
C Hou
Publications and source records attributed to C Hou.
[Study on platelet-derived growth factor mRNA and copper-zinc superoxide dismutase mRNA expression changes of aortic artery endothelium with restenosis after aortic artery dilatation treated with buyang huanwu decoction].
OBJECTIVE: To study on dynamic mechanism of restenosis after PTCA treated with Buyang Huanwu Decoction (BYHWD). METHODS: Twenty-eight male New Zealand rabbits with mimic restenosis after aortic artery dilatation were detected by prime in situ labeling in aortic endothelium. The items included gene expression of copper zinc superoxide dismutase (SOD-1) and platelet-derived growth factor receptor (PDGFR) mRNA to analyse dynamic mechanism of restenosis. RESULTS: Seven days after aortic artery dilatation, PDGFR mRNA of aortic endothelium decreased clearly. The comparison between experimental and the control group showed significant difference (P < 0.05). By the same condition, the gene expression of SOD-1 was increasing, which showed a significant difference with the control group (P < 0.05). CONCLUSIONS: BYHWD increased SOD-1 gene expression of artery endothelium, to improve oxygen feed for ischemia reperfusion in the same way, BYHWD suppressed PDGFR mRNA expression to control cellular pathological proliferation.
Angioplasty, Balloon, Coronary↗
[Chemical constituents of the roots of Glycyrrhiza eurycarpa P.C.Li].
Four compounds were isolated from the roots of Glycyrrhiza eurycarpa. They were identified as licochalcone A, glabrol, betulic acid and beta-sitosterol by chemical and physical properties and spectral analysis.
Chalcone↗
[In vitro experimental study of adriamycin-loaded chitosan drug delivery system].
Chitosan is a kind of biological material with good histocompatibility and gradual biodegradability in vivo. It has no toxicity or side-effect. For its gradual degradation, chitosan and adriamycin were mixed and formed drug delivery system (DDS). The release test of DDS and exudant of DDS in inhibiting OS-116 were examined in vitro. The results were as following: the DDS could release adriamycin in slow and stable way. The SO-116 inhidition rate of the exudant of the DDS on the 1st, 20th, 40th and 60th day was 58.11%, 36.48%, 24.32% and 21.62% respectively. It was concluded that the drug delivery system was a slow release system. It could maintain the concentration of adriamycin in a certain level. It was also suggested that the chitosan was a good carrier for slow release of chemotherapeutic drug in local therapy for postoperative treatment of bone tumor.
Antibiotics, Antineoplastic↗
[Advance in the study of restoration of sensation in skin flaps].
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Animals↗
[A preliminary report on clinical application of biopoly ester ligament].
Sixty-one cases of fracture or dislocation were treated with France-made biopolyester ligament. They consisted of 18 cases of fracture of patella, 13 cases of fracture of olecranon, 8 cases of fracture of distal clavicle, 10 cases of dislocation of acromino-clavicular joint, 6 cases of separation of lower tibio-fibular joint, 3 cases of rapture of cruciate ligament and 3 cases of fracture of upper third of ulna with dislolcation of radial head. The follow-up period lasted from 3 to 12 months with an average of 7.3 months. The result showed that of the 56 follow-up cases, 49 were excellent and 7 were satisfactory. There was no redisplacement occured in this group. The Biopolyester ligament was believed to be a good and safe material for fractures or dislocations and was of good strength of extension and easy to use.
Adolescent↗
The role of conserved histidine residues in the pyridine nucleotide transhydrogenase of Escherichia coli.
The pyridine nucleotide transhydrogenase of Escherichia coli catalyzes the reversible transfer of hydride ion equivalents between NAD+ and NADP+, coupled to translocation of protons across the cytoplasmic membrane. The role of histidine residues in catalysis was investigated by chemical modification with diethylpyrocarbonate and by site-directed mutagenesis. Diethylpyrocarbonate inhibited both hydride ion transfer and coupled proton translocation. Histidine residues were modified as shown spectroscopically and by the ability of hydroxylamine to cause reversal of inhibition. Complete inhibition of hydride ion transfer occurred following modification of 10 residues/enzyme molecule. Site-directed mutagenesis of single conserved histidine residues or the presence of substrates did not provide resistance to inhibition by diethylpyrocarbonate. It is concluded that diethylpyrocarbonate inhibition was a consequence of the structural changes brought about by modification of many histidine residues. With the exception of beta-subunit residue His91 (beta His91), in which mutation can result in specific loss of proton translocation activity [Glavas, N. A., Hou, C. & Bragg, P. D. (1995) Biochemistry 34, 7694-7702], site-directed mutation of the remaining conserved residues alpha His450, beta His161, beta His345 and beta His354 did not demonstrate a direct role for these residues in catalysis. Mutation of beta His161 had relatively little effect on the properties of the enzyme. By contrast, mutation of alpha His450, beta His345 and beta His354 caused major loss of enzyme activities which was probably due to alterations in the structure of the enzyme. These alterations were reflected in changes in the K(m) values for transhydrogenation.
Amino Acid Sequence↗
Localization of the origin of the atrioventricular junctional rhythm induced during selective ablation of slow-pathway conduction in patients with atrioventricular node reentrant tachycardia.
During radiofrequency catheter ablation of slow atrioventricular node pathway conduction in patients with atrioventricular node reentrant tachycardia, an atrioventricular junction rhythm is frequently observed. The origin and relation to ablation success of this junctional rhythm was examined in this study. By using standard intracardiac electrophysiology techniques, we studied the radiofrequency energy-induced atrioventricular junctional rhythm in 43 consecutive patients with atrioventricular node reentrant tachycardia undergoing selective ablation of slow-pathway conduction. The frequency of atrioventricular junctional activity was correlated with successful and unsuccessful attempts at ablation of slow-pathway conduction. Also, we compared the sequence of retrograde atrial activation of radiofrequency energy-induced atrioventricular junctional beats in a subgroup of 22 patients with the retrograde activation sequence observed during pacing from the right ventricular apex and the site of successful ablation of slow-pathway conduction. A total of 201 radiofrequency-energy applications was delivered in 43 patients with > or = 5 atrioventricular junctional beat(s) induced during 110 (55%) of 201 ablation attempts. Atrioventricular junctional activity was noted during 98% of successful ablations but only 43% of the unsuccessful attempts (sensitivity, 98%; specificity, 57%; negative predictive value, 99%). The mean time to appearance of atrioventricular junctional beats was 8.8 +/- 4.1 sec (mean +/- SD) after the onset of radiofrequency-energy application. In 22 (100%) of 22 patients in whom detailed atrial mapping was performed, the retrograde atrial activation sequence of the radiofrequency-induced atrioventricular junctional beats was earliest in the anterior atrial septum, identical to that seen during pacing from the right ventricular apex. Earliest retrograde atrial activation was at the posterior septum in all patients during pacing from the successful ablation site, a markedly different activation pattern compared with that seen during either radiofrequency ablation or ventricular pacing. Whereas the occurrence of atrioventricular junctional activity during radiofrequency ablation does not necessarily herald a successful ablation of slow atrioventricular node pathway conduction, its absence strongly suggests that the energy is being applied in an unsuccessful fashion. Furthermore, it appears that radiofrequency energy-induced atrioventricular junctional beats originate not from the endocardium in contact with the ablating catheter tip but instead appear to exit remotely from the anterior atrial septal region. This finding supports the existence of specialized tissues in the atrioventricular junction that preferentially transmit the effects of radiofrequency energy to an anterior exit site, possibly identical to the atrial exit site of the retrograde fast atrioventricular node conduction pathway.
Atrioventricular Node↗
[Chemical constituents of Swertia pubescens Franch].
Four compounds were isolated from Swertia pubescens. They were identified as isoorientin, gentiopicroside, glucose and oleanolic acid by chemical and physical properties and spectral analysis.
Drugs, Chinese Herbal↗
[Congenital anotia with hemifacial atrophy (a report of 12 cases)].
Twelve cases of congenital anotia with hemifacial atrophy were examined and analyzed with electromyogram and roentgenography. The results revealed that the development of the deformed side of the face was 30% less than the other side. Because both the soft tissue and the skeleton of the deformed side are undeveloped, they should be dealt with simultaneously.
Abnormalities, Multiple↗
Organization in the membrane of the N-terminal proton-translocating domain of the beta subunit of the pyridine nucleotide transhydrogenase of Escherichia coli.
The proton-translocating transmembrane pyridine nucleotide transhydrogenase of Escherichia coli is composed of two types of subunits, alpha and beta. The beta subunit has several membrane-spanning segments in the N-terminal region followed by a cytosolic C-terminal domain bearing a binding site for NADP(H). The N-terminal region contains at least one residue involved in the process of transmembrane proton translocation. Using site-directed mutagenesis cysteine residues were introduced at selected sites into the N-terminal region of the beta subunit. The pattern of labelling of these residues with 3-(N-maleimidyl propionyl)biocytin and other sulfhydryl reagents has shown that a model in which the N-terminal region of the beta subunit spans the membrane in eight segments is more likely than a previously proposed six segment model (Holmberg et al. (1994) Biochemistry 33, 7691-7700). The preferred model accounts for the site of labelling of a glutamate residue (Glu124) in the N-terminal domain by N,N-dicyclohexylcarbodiimide.
Amino Acid Sequence↗
Involvement of histidine-91 of the beta subunit in proton translocation by the pyridine nucleotide transhydrogenase of Escherichia coli.
The pyridine nucleotide transhydrogenase (EC 1.6.1.1) carries out transmembrane proton translocation coupled to transfer of a hydride equivalent between NAD+ and NADP+. Mutations were made in histidine-91 of the beta subunit of the pyridine nucleotide transhydrogenase of Escherichia coli. This amino acid is the only conserved charged residue in the transmembrane domains of this enzyme and thus potentially is involved in proton translocation by the transhydrogenase. The mutant beta H91N retained 80% of the hydride transfer activity while proton translocation was reduced to 7%. This behavior is consistent with a role for beta His91 in the proton translocation pathway. Other mutations at this residue affected the conformation of the enzyme. Thus, the enzyme in mutants beta H91C, beta H91T, and beta H91S was unable to undergo the conformational change that occurred on binding of the substrates NADP+ or NADPH. By contrast, the enzyme in the beta H91K mutant was present in the NADP(H)-induced conformation even in the absence of these substrates. Further evidence for the linkage between beta His91 and the conformation of the beta subunit was obtained by labeling the transmembrane domain of the beta subunit with [14C]N,N'-dicyclohexylcarbodiimide (DCCD). Labeling occurred most readily with the enzyme of beta H91K. It is concluded that beta His91 is a component of the proton translocation pathway of the transhydrogenase and that its state of protonation is probably linked to conformational changes induced by binding/debinding of substrates during the catalytic cycle of the enzyme.
Amino Acid Sequence↗
Double cortical line in the acetabular roof of paraplegic patients.
The pelvic radiographs of 60 patients with paraplegia or quadriplegia were reviewed and double cortical line in the acetabular roofs on both sides was found in 35 patients. It was concluded that the double cortical line was a radiologic sign of disuse osteoporosis after spinal cord injury. It was noticed that the acetabular double cortical line in incomplete paralysis was not so prominent as that in complete paralysis (P < 0.05), suggesting that the degree of acetabular osteoporosis may be related to the severity of spinal cord injuries. The etiology of double cortical line and related factors were discussed.
Acetabulum↗
[Isolation, purification and characterization of human proteinase inhibitor, alpha 2-macroglobulin].
A study of the isolation, purification and characterization of human proteinase inhibitor, alpha 2-macroglobulin (alpha 2-M) is presented. The alpha 2-M, with a purity of more than 90% and subunit molecular weight of 185kDa, was isolated by ammonium sulfate fractionated precipitation from human blood plasma and purified by DEAE cellulose DE52 column chromatography. It was found to be the same as the standard counterpart provided by sigma company. This purification procedure of alpha 2-M can be done with low cost, high efficiency and in large scale preparation.
Chromatography↗
[Anti-lipid peroxidation effects of sini decoction and its components on ischemic myocardium and the dose- and time-effects].
The complete Sini Decoction can significantly reduce the content of MDA in ischemic myocardium. Single ingredients of the decoction, such as Radix Aconoti Praeparata or Radix Glycyrrhizae, except Rhizoma Zingiberis, also have similar effect but inferior to that of the complete decoction. Under the present conditions of experiment, anti-lipid peroxidation of Sini Decoction is significant at doses ranging from 0.05 to 0.2ml/20g administered for 2-7 days at 0.1ml/(20g.d).
Animals↗
[Experimental observations of the quantitative changes of proteoglycan in homogenous cartilage graft in the guinea pig].
The present work was to study the quantitative change of proteoglycan (PG) in homogenous cartilage graft in the guinea pig. Histological, histochemical and biochemical studies were undertaken at different times after grafting. The results revealed that there was a marked reducing trend of matrix PG within four weeks after grafting. Four weeks afterwards all indexes except hexuronic acid restored slowly and approached the level of the control group. By the 20th week, the activity of neutromucopolysaccharide, hexosamine, acid-mucopolysaccharide and alkaline phosphatase restored to 73.6% -93.7% of the control group (P > 0.05). The changes probably were caused by immune reaction and operative trauma. PG in the cartilage matrix forms a barrier for the chondrocytes, protecting the host's immune system from contacting and reacting with the chondrocytes of the graft. Though the core protein of PG has antigenicity, it is surrounded by acidmucopolysaccharide that has no antigenicity. We concluded that it is very important to maintain a high level of PG in order to prolong the survival of a cartilage graft.
Animals↗
The analysis of disparity evoked potentials by a new form of static random-dot stereograms.
PURPOSE: A new form of static random-dot stereograms free of monocular clues was designed as stimulus to elicit disparity evoked potentials. METHODS: Disparity evoked potentials were recorded in 40 stereo-normal subjects. The stimulator was a white-black static random-dot stereograms generated by a computer and had no monocular clues. Every subject was tested in disparity stimulus, zero disparity stimulus, monocular stimulus and wearing prism condition. RESULTS: A characteristic wide positive wave at about 250 ms was consistently recorded in disparity stimulus, which may be regarded as evidence of the presence of stereopsis. In contrast, recordings for zero disparity stimulus, monocular vision, stimulus and wearing prism condition all demonstrated a markedly difference from recording for disparity stimulus.
Adolescent↗
[Progress on research in preventing and treating the myocardial ischemia/reperfussion injury with Chinese herb medicine].
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Animals↗