Previous studies have shown that the BM88 antigen, a neuron-specific molecule, promotes the differentiation of mouse neuroblastoma cells [23] (Mamalaki A., Boutou E., Hurel C., Patsavoudi E., Tzartos S. and Matsas R. (1995) The BM88 antigen, a novel neuron-specific molecule, enhances the differentiation of mouse neuroblastoma cells. J. Biol. Chem. 270, 14201-14208). In particular, stably transfected with the BM88 cDNA, Neuro 2a cells over-expressing the BM88 antigen are morphologically distinct from their non-transfected counterparts; they exhibit enhanced process outgrowth and a slower rate of division. Moreover, they respond differentially to growth factors [10] (Gomez J., Boutou E., Hurel C., Mamalaki A., Kentroti S. , Vernadakis A. and Matsas R. (1998) Overexpression of the neuron-specific molecule BM88 in mouse neuroblastoma cells: Altered responsiveness to growth factors. J. Neurosci. Res. 51, 119-128). In order to further elucidate the role of the BM88 antigen in the differentiation of developing neurons we used the in vitro system of differentiating P19 cells which closely resembles early murine development in vivo. In this study, P19 cells were driven to the neuronal pathway with retinoic acid. We examined by immunofluorescence studies the expression of the BM88 antigen in these cells and we found that it correlates well with the expression of the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) which characterizes early differentiating post-mitotic neurons. In contrast, very few of the BM88 antigen-positive/PSA-NCAM-positive cells expressed neurofilament protein, a marker of more mature neurons. Our findings, in accordance with previously reported data, strongly suggest that the BM88 antigen is involved in the early stages of differentiation of neuronal cells.

Animals↗

PubMedDate not supplied

[The first permanent molars and palatal or mandibular variations].

101 children, each of them with two teleradiographies, were selected. The first X-ray was taken at the time of the mixed dentition, the second one, when the permanent dentition was established. The purpose of the research is to show the oscillations of the palatine and mandibular planes, and their link to the movements of the first permanent molars. The palatine plane is found to swing between -7 degrees and +5 degrees with an average of -0.97 degree. The posterior part of that plane rocks downwards in 51% of the children. The limits are -7 degrees and -1 degree, with an average of -3.2 degrees. In 22%, the plane rocks upwards between +1 degree and +5 degrees with an average of +3.1 degrees. In 27% it moves parallel to itself. The posterior part of the mandibular plane varies between -6 degrees and +5 degrees, with an average of -1.1 degrees. It rocks downwards in 48% of the cases, between -6 degrees and -1 degree with an average of -3.9 degrees. In 22% of the children, this plane rocks upwards between +1 degree and +5 degrees with an average of +2.5 degrees. In 30%, it moves parallel to itself. The overall result is that the more the posterior part of the palate, or of the mandible moves downwards, the more the first permanent molars get straight or move forwards.

Child↗

PubMedDate not supplied

[Movement of the permanent 1st molars in children].

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Adolescent↗