PubMed Health⌕ Search

Biomedical subjects

C Imai

Publications and source records attributed to C Imai.

At least 37 records · Page 2Linked to original sources
PubMed1989

Cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti.

A genomic library of Acetobacter aceti DNA was constructed by using a broad-host-range cosmid vector. Complementation of a spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation of a plasmid designated pAA701. Subcloning and deletion analysis of pAA701 limited the region that complemented the deficiency in alcohol dehydrogenase activity of the mutant. The nucleotide sequence of this region was determined and showed that this region contained the full structural gene for the 72-kilodalton dehydrogenase subunit of the alcohol dehydrogenase enzyme complex. The predicted amino acid sequence of the gene showed homology with sequences of methanol dehydrogenase structural genes of Paracoccus denitrificans and Methylobacterium organophilum.

Acetobacter↗
PubMed1988

Tissue-type plasminogen activator inhibits aggregation of platelets in vitro.

The effects of tissue-type plasminogen activator (t-PA) on the platelet aggregation were studied using citrated whole blood and platelet-rich plasma (PRP) obtained from human donors. t-PA suppressed adenosine 5'-diphosphate (ADP)- or collagen-induced platelet aggregation in a dose-dependent manner. The 50% inhibitory concentration (IC50) for t-PA was lower by one order of magnitude than that for urokinase (UK) in whole blood and PRP. The suppression of platelet aggregation was not completely inhibited by alpha-2-antiplasmin. t-PA did not cause the degradation of fibrinogen or fibrin in PRP, whereas UK caused the reduction of fibrinogen and fibrin, and the increase of fibrinogen- and fibrin-degradation products (FDP). These results suggest that the mode of action of t-PA in inhibiting platelet aggregation may be different from that of UK.

Adenosine Diphosphate↗
PubMed1988

Egg yolk paste for determining some food poisoning bacteria.

Egg yolk, aseptically prepared from fresh eggs, was partially dehydrated with a 40% high fructose corn syrup solution, and 10% salt was added. This salted yolk paste was added to mannitol salt agar for the detection of Staphylococcus aureus, to NaCl-glycine Kim and Goepfert medium for detection of Bacillus cereus, to Clostridium welchii agar for detection of C. perfringens, and to Gifu anaerobic medium for detection of C. botulinum. These food poisoning bacteria showed the same lecithovitellin (LV) reaction on these media as on the same media prepared with fresh egg yolk. The yolk paste could be stored at -20 C without freezing and did not show any bacterial growth after holding at 25 C for 30 days. The increased salt content resulted from the addition of salted yolk paste to the media did not inhibit the growth of the food poisoning bacteria used in these experiments. For the identification of the food poisoning bacteria used in this work, and which give a LV reaction, salted yolk paste is more convenient to use than yolk separated from fresh shell eggs.

Bacillus cereus↗
PubMed1985

[Analysis of human sera obtained from lung cancer patients by two-dimensional electrophoresis after schizophyllan (SPG) treatment].

Schizophyllan (SPG) was administered to 13 lung cancer patients (i.m. 20mg X 2/week) for 3 weeks without chemo or irradiation therapies, and serum proteins were analyzed by two-dimensional electrophoresis (TDE). Additionally, immunosuppressive acidic protein (IAP) was quantitatively determined by single radial immunodiffusion (SRID). By TDE analysis, human serum proteins were separated into more than 100 spots, and about 14 spots were found to show quantitative changes in cancer patients. Quantitative examination was therefore conducted on changes of 8 components among these spots, including alpha 1-acidic glycoprotein (alpha 1 AG), acidic alpha 2-macroglobulin (acidic alpha 2 M), haptoglobin (Hp) and IAP. The protein which showed the most marked decrease in cancer patients, located between transferrin and IgG on the above TDE patterns, was ascertained to have a molecular weight of about 150,000 using a gel filtration method. This protein was increased in 7 of 13 patients after SPG treatment.

Adenocarcinoma↗
PubMed1980

Ileal uptake of oleic acid: evidence for adaptive response to high fat feeding.

When I-14C oleic acid at 120 micron Eq/hr was infused into the duodenum in normal rats in a micellar solution with mono-olein (60 mu moles/hr) in 15 mM taurocholate over 6 hr uptake was nearly complete (97%). However, when this same solution was infused into the mid small bowel in control animals uptake was incomplete (88.9 +/- 2.6%, mean +/- SEM, P < 0.01). After 4 weeks on a high fat diet, containing 45% vegetable oil by weight, oleic acid uptake increased to 98.1 +/- 0.1% (P < 0.01 compared to controls). The improved uptake of oleic acid was associated with increased dry weight of mucosa in the proximal half of the ileum from 109 +/- 8.8/20 cm in controls to 135.6 +/- 7.3 mg/20 cm in high fat diet fed rats (P < 0.05), while protein increased from 107.4 +/- 6.5 to 124.9 +/- 4.8 mg/20 cm (P < 0.05). There was no increase in DNA expressed as mg/g wet weight of mucosa or in number of cells per villus. Lipid content of the mucosa and degree of esterification of absorbed oleic also were unaltered. These results indicate that the mucosa of the proximal ileum responds to high fat feeding by hypertrophy (increased mass and protein, with no change in DNA content or cell number) and that this change is associated with more complete uptake of oleic acid reaching this part of the small bowel.

Adaptation, Physiological↗
PubMed1980

Behavior of enterococci in egg processing operations.

Since enterococci were detected in dried and frozen egg products (whole egg and egg yolk), the origin of the enterococci and their behavior in the different stages of egg processing were surveyed. The bacterial survey of unwashed and washed eggs, gathered from several parts of Japan, showed the presence of an average of 60 enterococci per egg on the shell surface of unwashed eggs. Smaller numbers of enterococci were detected on the shell surface of washed eggs. Most of the detected enterococci were Streptococcus faecalis and S. faecalis var. liquefaciens. The contents of two eggs were contaminated with enterococci when 120 washed eggs were examined. Enterococci were destroyed to some extent by the disinfectants used for washing the shell eggs, but they seemed to have greater resistance than Escherichia coli and Pseudomonas fluorescens used for comparison. In whole egg and egg yolk, enterococci grew rapidly at 25 C, slowly at 10 C, and very slowly at 2.5 C. The frozen storage of both whole egg and egg yolk at -20 C for 3 months only slightly decreased the number of enterococci. They were decreased only slightly by normal pasteurization but were destroyed in the desugaring process by the glucose oxidase method. Enterococci numbers were only slightly decreased by spray-drying whole egg and yolk. Considering these characteristics of enterococci, it appears to be difficult to produce enterococci-free egg products in a normal production line.

Animals↗