Biomedical subjects

C J David

Publications and source records attributed to C J David.

4 recordsLinked to original sources

PubMed2001

Determination of human beta(2)-adrenoceptor haplotypes by denaturation selective amplification and subtractive genotyping.

OBJECTIVE: beta(2)-Adrenoceptor haplotype may be better associated with asthma severity and drug response than a polymorphic variant at any single site. Because present methods of haplotype determination are time consuming and impractical for large population studies, we sought to develop a simple and efficient method of determining haplotype of 3 common polymorphisms at codons -19 (Arg/Cys), 16 (Arg/Gly) and 27 (Glu/Gln). DESIGN: Preliminary studies showed that the C/G base pair of the Arg(-19) allele increases the local melting temperature over the T/A base pair of the Cys(-19) allele by 3.6 degrees C and establishes a new local maximum denaturation temperature. By choosing a suitable denaturation temperature and appropriate primers and coupling them with restriction fragment length polymorphism (RFLP) analysis, we hypothesized that the genotype of one separately amplified allele followed by subtraction from the combined genotype of two alleles would yield the beta(2) haplotype in > 99% of the population. RESULTS: Haplotype determined by our method was in complete agreement with haplotype determined by cloning and sequencing in 29 samples. The frequencies of haplotype pairs in 78 healthy adults, according to our method, were in agreement with published values that were inferred, and were: RGE/CRQ, 26.9%; CRQ/CRQ, 25.6%; RGE/CGQ, 16.7%; CRQ/CGQ, 10.3%; RGE/RGE, 11.5%; CGQ/CGQ, 7.7%. The haplotype pair in one individual was RRE/CRQ (1.3%). CONCLUSION: Our method of determining beta(2)-adrenoceptor haplotype is simple, accurate and cost effective for haplotyping large populations.

DNA Primers↗

PubMed1982

Renal lymphoma: radiologic-pathologic correlation of 21 cases.

The radiologic findings in 21 cases of renal lymphoma were correlated with the gross and microscopic pathologic findings. The gross morphology and consequent radiographic images depend upon the mechanism of renal involvement (hematogenous or direct extension); the pattern of intrarenal growth (interstitial or expansile); the size, number, and distribution of lesions; and the presence of extension beyond the kidney. Initially, the lymphoma grows between nephrons that may continue to function and, because this interstitial proliferation preserves gross morphology, radiographic detection may be difficult. With continued growth, the scaffolding along which the tumor infiltrates is destroyed, growth becomes expansile, and the lymphomatous mass resembles other renal neoplasms that enlarge by appositional growth. Continued growth and coalescence of small foci result in progressive parenchymal replacement and, rarely, in destruction of the entire kidney. Perinephric extension with subsequent vascular and ureteral encasement is common.

Adolescent↗

PubMed1980

The resurrection-of-the-dead syndrome.

The purpose of this article is to illustrate some of the psychological responses of key family members toward a patient who has serious medical illness and who has survived beyond the prognosticated time due to heroic intervention of medical technology or due to a new procedure becoming available. The phenomena described have customarily been under the heading of anticipatory grief but in some instances go beyond grief so that, in the mental representation of the family member, the physically sick member is perceived as 'not being alive' and is treated as though he or she were dead. This may lead to covert or overt rejection or actual sabotage of planned treatment for the patient. Recognition of these attitudes may help prevent such final and total rejection of the patient by his family and has broad ramifications as to the actual process of communication of prognosis of the illness to family members.

Adolescent↗

PubMed1975

Grief, mourning, and pathological mourning.

An individual may present with a multitude of symptoms following an acute loss. Helping the patient go through the essential "grief work" may prevent prolonged and serious alterations in social adjustment as well as potential medical disease.

Adaptation, Psychological↗