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C J Drake

Publications and source records attributed to C J Drake.

23 records · Page 2Linked to original sources
PubMed1990

Avian vasculogenesis and the distribution of collagens I, IV, laminin, and fibronectin in the heart primordia.

The heart-forming regions of the early embryo are composed of splanchnic mesoderm, endoderm, and the associated ECM. The ECM of the heart-forming regions in stage 7-9 chicken embryos was examined using immunofluorescence. Affinity purified antibodies to chicken collagens type I and IV, chicken fibronectin, and mouse laminin were used as probes. We report that (1) the basement membrane of the endoderm contains immunoreactive laminin and collagen IV; (2) the nascent basement membrane of the heart splanchnic mesoderm contains immunoreactive laminin, but not type IV collagen, and (3) the prominent ECM between the splanchnic mesoderm and the endoderm (the primitive-heart ECM) contains collagen IV, collagen I, fibronectin, but not laminin. In addition, we describe microscopic observations on the spatial relationship of cardiogenic cells to the primitive-heart ECM and the endodermal basement membrane.

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PubMed1989

Distribution of laminin, collagen type IV, collagen type I, and fibronectin in chicken cardiac jelly/basement membrane.

Light microscopic immunolabeling studies were designed to identify and locate structural components within the cell-free extracellular matrix which lies between the embryonic endocardial and myocardial tubes. Affinity-purified antibodies were used to examine stage 15-22 embryonic chicken hearts. Specimens were immunolabeled by using three different methodologies: 1) postembedding labeling of 10 microns cryostat sections, 2) preembedding labeling (en bloc) of whole hearts, and 3) postembedding labeling of ethanol/acetic acid-fixed paraffin sections. Our results establish the spatial distribution of collagen type I and demonstrate for the first time the presence of collagen type IV and laminin in the myocardial-basement-membrane/cardiac jelly.

Animals↗
PubMed1988

A survey by scanning electron microscopy of the extracellular matrix and endothelial components of the primordial chick heart.

Scanning electron microscopy was used to study the morphogenesis of the primitive embryonic chick heart (stage 5 late primitive streak through stage 9+). Components of the developing heart (myocardium, endocardial endothelium, and extracellular matrix) were viewed from the ventral surface after removal of the endoderm. The myocardial component of the heart can first be seen by light microscopy at stage 5 as two darker oval-shaped areas located on either side of the embryonic axis in the cranial region of the embryo. Scanning electron microscopy demonstrates that as early as stage 6 an area of extracellular matrix, enriched in comparison to more lateral and medial splanchnic mesoderm, can be identified ventral to the myocardial primordium. As heart formation progressed we observed primordial endothelial elements in the splanchnic mesoderm lateral to the myocardial primordia. By late stage 7 these lateral primordial elements had anastomosed into small, loose plexuses. This process of anastomosis progressed rapidly, and by stage 8 the entire cranial surface of the myocardial primordium was covered with vascular plexuses. By late stage 8 the progressive fusion of these plexuses resulted in the formation of large multiple tubular elements near the midline. More medially the fusion of tubular elements resulted in a continuous endothelial sheet at the midline.

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