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Biomedical subjects

C J Meyer

Publications and source records attributed to C J Meyer.

At least 55 records · Page 3Linked to original sources
PubMed1983

Adult T-cell leukemia: a report on two white patients.

Two white European males are reported with adult T-cell leukemia (ATL), a disease first described in Japan, but recently also in the U.K. and U.S.A. Both patients presented with lymphadenopathy, but without a mediastinal mass. In addition, one patient had skin infiltrates and the other had hepatosplenomegaly. Morphologic and ultrastructural examination of the blasts in bone marrow and lymph node biopsy revealed a predominance of polymorphic lymphoid cells with pronounced nuclear irregularities and a semi-mature chromatine pattern. Histopathology of the lymph nodes showed a diffuse infiltration with medium-sized lymphoblasts with irregular nuclei. The blasts in the bone marrow formed E rosettes with sheep erythrocytes, lacked terminal deoxynucleotidyl transferase (Tdt) activity but expressed the Ia-like antigen; although the majority of the cells reacted with a polyclonal anti-T-cell serum, they were negative for OKT3. In one patient a helper/inducer phenotype (OKT4+) was found in the lymphoblasts of bone marrow and lymph node, while in the other only in the lymph node. The difference between bone marrow and lymph node phenotype is discussed. To our knowledge, these are the first two European patients reported with ATL, a disease clearly different from convoluted T-cell acute lymphocytic leukemia.

Adult↗
PubMed1982

The clinical and histological spectrum of lymphomatoid papulosis.

A review of sixty-four biopsies from sixteen patients with lymphomatoid papulosis revealed two characteristic histological types (type A and type B), which also had a different clinical behaviour. In lymphomatoid papulosis type A lesions, four histological patterns corresponding with the age of the lesion could be distinguished. Such a relationship was not found in type B lesions. The findings of transitional forms in some biopsy specimens, showing histological features of both type A and type B, and the presence of both types in different but concurrent lesions, suggests that these two types are not different entities but rather represent the ends of a spectrum. At least two different populations of atypical cells can be distinguished in lymphomatoid papulosis. Apart from the atypical cerebriform mononuclear cells, which are T-lymphocytic in origin and predominant in type B lesions, large atypical cells with vesicular nuclei, prominent nucleoli and abundant cytoplasm are found, particularly in type A lesions. Preliminary immunohistochemical and cytochemical investigations suggest that these cells are not lymphoid in origin, but are related to the Langerhans cell series.

Adult↗
PubMed1982

Ultrastructural localization of the low molecular weight protease inhibitor in human bronchial glands.

Light microscopically the low molecular weight protease inhibitor (LMI) and lysozyme have both been demonstrated in the cytoplasm of serous cells of bronchial glands. By immunoelectron microscopy LMI was demonstrated in secretory granules of these serous cells. Many granules showed a peripheral or bean-shaped staining pattern, other granules were uniformly stained or not stained at all. In contrast to this latter finding, virtually all granules were found positive for the presence of lysozyme, suggesting that there is no association of LMI with lysozyme at the ultrastructural level.

Bronchi↗
PubMed1981

Staging lymphography with respect to lymph node histology, treatment, and follow-up in patients with mycosis fungoides.

Extracutaneous involvement in mycosis fungoides (MF) is associated with a poor prognosis. Therefore, a pretreatment staging procedure is used to determine the extent of the disease. As part of the staging procedure lymphography was performed in 36 patients with MF. The radiographic findings and the correlation of these findings with the histopathologic changes in lymph nodes and the clinical course are described. Lymphography in MF shows a variety of abnormalities. However, these abnormalities correlate in only 61% of the patients with the extent and the clinical course of the disease. It is concluded that lymphography is not a useful clinical tool in the staging of MF.

Adult↗
PubMed1981

Benign lymphoepithelial lesion and malignancy.

Whereas most patients with benign lymphoepithelial lesion suffer from the involvement of a major salivary gland a number evolve into a clinical form of Sjögren's syndrome or Mikulicz's disease. In a small number development of malignant lymphomas, especially non-Hodgkin's lymphomas, have been described. Therefore, regular follow-up and appropriate histological evaluation of suspected areas in all patients with a benign lymphoepithelial lesion is indicated. Histologically, diagnosis of a non-Hodgkin's lymphoma may be difficult. Demonstration of a cell pattern, monoclonal for cytoplasmic Ig by means of the immunoperoxidase technique may facilitate the diagnosis. In this report we present the history of two cases out of our series with benign lymphoepithelial lesion who developed a non-Hodgkin's lymphoma.

Aged↗
PubMed1981

Primary lymphoblastic non-Hodgkin's lymphoma of the skin.

The clinical, histological, and ultrastructural features of a patient with a primary lymphoblastic non-Hodgkin's lymphoma of the skin are described. Four months after the onset of the disease the patient died with extracutaneous involvement of lymph nodes, spleen, liver, kidneys and lungs. The rapidly fatal outcome without the development of leukaemia or involvement of the bone marrow and the mediastinum is uncommon.

Humans↗
PubMed1981

Inhibitory effect of molybdenum and vanadium salts on aflatoxin B1 synthesis by Aspergillus flavus.

The effects of the elements zinc, manganese, iron, copper, molybdenum, and vanadium, added in various salt forms, on mycelial weights and aflatoxin B1 accumulation in the mycelium of Aspergillus flavus were investigated in liquid shake cultures. Ammonium heptamolybdate, when added to a complete medium at concentrations of 50-100 mg/L, appreciably reduced aflatoxin B1 accumulation without affecting growth of the fungus. Sodium molybdate and sodium monovanadate also reduced aflatoxin B1 yields without affecting mycelial growth but to a lesser extent. The addition of zinc sulphate stimulated aflatoxin B1 production in all media used. The influence of the other trace elements on aflatoxin production depended on the level of trace elements present in the basal medium. In general, manganese chloride had a stimulatory effect, whereas copper sulphate depressed yields. Mycelial levels of aflatoxin had peaked and then declined before mycelial dry weights had reached maximum. High yields of aflatoxin B1 were obtained in media having a final pH as low as pH 2.8.

Aflatoxin B1↗
PubMed1980

Field outbreaks of hyperoestrogenism (vulvo-vaginitis) in pigs consuming maize infected by Fusarium graminearum and contaminated with zearalenone.

During the spring and summer of 1979 field outbreaks of porcine hyperoestrogenism characterized by swelling and reddening of the vulva and teats in prepubertal gilts and enlargement of the mammae in young males occurred in the mistbelt of the Natal Midlands. On a farm in the Ixopo district, pigs were fed a mixed ratio containing home-grown yellow maize stored on the cob in a crib and all the young pigs with a mass of 25-85 kg (=a total of 350) were affected. Maize ears from the crib had a high percentage (91%) of kernels infected by Fusarium graminearum and contained 10 mg/kg of zearalenone. The mixed ration contained 0,95 mg/kg zearalenone. After dilution of the contaminated maize with good quality white maize, a dramatic decrease in both the incidence and severity of clinical signs occurred within 3-4 days. On a farm in the Winterton district, approximately 80% of the growing pigs with a mass of more than 40 kg fed a mixed ration containing shelled yellow maize from the silo of the local Agricultural Co-operative were affected. The incidence as well as the severity of the swelling of the vulvas and/or treats were lower than on the farm at Ixopo, thus indicating a lower level of oestrogen intake. A sample of maize from the silo delivered to this farm contained only 0,06 mg/kg zearalenone while the mixed ration did not contain chemically detectable levels of zearalenone. This is the first published record of field outbreaks of porcine hyperoestrogenism associated with the ingestion of F. graminearum infected maize contaminated with zearalenone in the Republic of South Africa.

Animal Feed↗
PubMed1978

A morphometrical analysis of lymph node responses to tumors of different immunogenicity.

An immunogenic tumor and a nonimmunogenic tumor were used in C57BL/Ka and CBA/Rij mice, respectively, in a study in which lymph node morphology was quantitatively measured. Lymph nodes were cut in semiseries, and a morphometrical analysis was done on the paracortical and cortical areas as major parts of the active lymph nodes. These parameters could be expressed with confidence in both absolute and relative numbers, since a strong correlation existed between the weight of lymph nodes and the summated surface areas. To determine whether the regional lymph node plays a major role in this response, we performed similar analyses of two nonregional lymph nodes. It was observed that immunogenicity, defined as the ability to evoke an immune response that significantly influences tumor cell take and tumor cell growth, was not relevant in evoking a morphologically evident response. In absolute numbers, the response evoked was strongest in the regional lymph nodes in both systems. However, the percentages of paracortical and cortical areas in the lymph nodes reacting were not essentially different from one node to another during the growth of both tumors. This observation was confirmed by an analysis of variance. These findings suggest that great caution must be exercised when one is interpreting morphological lymph node changes as evidence of an influence of immunological reactions on tumor cell growth.

Adenocarcinoma↗
PubMed1978

Determination of drugs in plasma by high-performance thin-layer chromatography.

High-performance thin-layer chromatography was used to determine chlorpromazine, amitriptyline, nortriptyline, imipramine, desipramine, phenobarbital, and phenytoin in plasma, to demonstrate the utility of this technique for routine analysis. We quantitated the separated components by use of ultraviolet reflectance spectrometry with detection limits as low as 1 microgram/liter. Regressions of psychoactive agents extracted from plasma were linear over the range of 0 to 300 microgram/liter. The anti-convulsant drugs, phenobarbital and phenytoin, were determined over a range of 0 to 50 mg/liter. Analyses were rapid, reproducible, and well-suited to large-scale programs. Separated components also can be identified in situ by ultraviolet spectrophotometry.

Amitriptyline↗
PubMed1977

High-performance thin-layer chromatographic determination of psychopharmacologic agents in blood serum.

High-performance thin-layer chromatography affords a rapid, sensitive method for determination of psychopharmacologic agents in blood serum samples. Quantitation of the representative drugs chlorpromazine, amitriptyline, nortriptyline, imipramine, and desipramine at levels as low as 5 ng/ml is demonstrated by scanning the developed thin-layer plates with a chromatographic spectrophotometer in the ultraviolet absorption mode. Neither derivatization prior to, nor color development after chromatographic separation is required to achieve sensitivity and reproducibility of determinations.

Amitriptyline↗
PubMed1977

Cerebriform (Sézary like) mononuclear cells in healthy individuals: a morphologically distinct population of T cells. Relationship with mycosis fungoides and Sézary's syndrome.

The ultrastructural and surface marker characteristics of lymphocytes in human cord blood and peripheral blood of healthy donors were studied with respect to the presence of cerebriform mononuclear cells similar to those occurring in the dermal infiltrate of patients with mycosis fungoides (mycosis cells), and the skin infiltrate and peripheral blood of patients with Sézary's syndrome (Sézary cells). Cerebriform monuclear (Sézary-like) cells are characterized by a high nucleus-cytoplasm ratio, deep and narrow nuclear identations, condensed chromatin at the nuclear membrane and cytoplasm poor in organelles. Of the lymphoid cells in human cord blood and peripheral blood of healthy donors 6.7 and 8.7% respectively proved to be cerebriform mononuclear cells. Since these cells invariably form E-rosettes they are part of the T-cell population in healthy individuals. The finding of similar cells in the skin infiltrate of patch test areas of patients allergic to rubber, formalin and peruvian balsam--an expression of cellular immunity mediated by T-cells--suggests that these cells are reactive T cells. Not all (up to 85%) of the cerebriform mononuclear cells in patients with mycosis fungoides and Sézary's syndrome have T-cell membrane characteristics as shown by E-rosette formation. This suggests the presence of two populations of cerebriform mononuclear cells in mycosis fungoides and Sézary's syndrome. The relationship of cerebriform T cells as seen in healthy individuals with cerebriform or atypical mononuclear cells occurring in the Sézary syndrome and mycosis fungoides is discussed.

Adolescent↗
PubMed1977

Improved gas chromatographic analysis of chlorpromazine in blood serum.

Reliable determinations of chlorpromazine levels in blood serum samples obtained from patients were accomplished by electron capture gas chromatography. By using modifications of the procedure to insure stability of the sample, minimal losses during sample preparation and gas chromatography, and by selecting appropriate operating parameters of the electron capture detector, excellent agreement was obtained in replicate analyses with a limit of sensitivity of 1 ng/ml in 1 ml of plasma.

Chlorpromazine↗
PubMed1977

Atypical T cells in rheumatoid synovial membranes.

Atypical mononuclear cells (AMC) with Sézary-cell-like morphology were demonstrated to be present in rheumatoid synovial membranes. These cells have exclusively T cell membrane characteristics, like identical cells previously found in rheumatoid synovival fluids. AMC were predominantly present in transitional areas between nodular lymphocytic aggregates and plasma cell rich areas. In ultrathin sections AMC accounted for approximately 30% of all lymphocyte cells; in studies of cells isolated from rheumatoid synovial membranes AMC comprised 30--40% of the E-rosette-forming cells. The hypothesis that these cells represent reactive T cells is discussed.

Arthritis, Rheumatoid↗