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C J Stratton

Publications and source records attributed to C J Stratton.

25 records · Page 2Linked to original sources
PubMed1976

The three-dimensional aspect of mammalian lung multilamellar bodies.

The three-dimensional aspect of rat and monkey lung multilamellar bodies was demonstrated in lipid retained thin sections. The glutaraldehyde and urea lipid retention embedment and an Epon 812 resin polar dehydrant procedure were utilized to retain lamellar lipids for precise morphological study. The unextracted multilamellar bodies were found to conform to a general, though complex, three-dimensional structure. A model that demonstrated that structure was derived. Freeze-etch and extracted material were shown to support the model. Mature multilamellar bodies were from 1-2-1-6 mu in diameter and were 1-0-1-6 mu high. Each body contained a matrix core that included from 2-25 vesicular bodies and was in contact with the limiting membrane at the matrix plate. Most bodies had from 25-70 lamellae attached for 360 degrees to the projection plate. Microtubules were seen in communication with the matrix core. When sectioned in longitudinal section, lamellae projected from the base plate and coursed parallel to the limited membrane of the top half of the body. Any cross-section produced circular lamellae without apparent attachment. Oblique sections sometimes produced both 'stacked' and 'circular' lamellae. Four postulates of multilamellar body formation were discussed in light of these findings.

Animals↗
PubMed1976

The high resolution ultrastructure of the periodicity and architecture of lipid-retained and extracted lung multilamellar body laminations.

High resolution electron microscopy was utilized to study the periodicity and architecture of rat and monkey, lung multilamellar bodies. In lipid retention embedments and polar dehydrants, the lamellar width (LW) was defined as a lipid bilayer. The osmiophilic band that contained two layers of phospholipid heads (PH) of adjacent lipid bilayers in immediate opposition and the translucent band or fatty ah = 35-38 a, fa = 31 a; hydroxyethyl methacrylate glutaraldehyde and urea embedment or fatty acid tail layer(FA) were also measured: glutaraldehyde and urea embedment, LW=66 A, PH=35-38 A, FA=37 A; Epon 812 resin polar dehydrant, LW=66 A, PH=35-38 A, FA=31 A; hydroxypropyl methacrylate polar dehydrant, semihomogeneous matrix without laminations. There was no interlamellar space, so that lamellar width was also the periodicity. In slightly extracted material the PH layer was usually the site of extraction. These results were considered in light of the previously reported measurements of extracted, lipid retained and frozen etched material. In vivo lamellae are probably 66 A wide, composed of 35-38 A PH and 31 A FA layers and do not exhibit an interlamellar space; Lamellae were observed to fracture at the FA layer at bends, to bifurcate and to anastamose.

Animals↗
PubMed1976

The investigation of vinylcyclohexane dioxide as a polar dehydrant for the improved retention of lipids in the Spurr embedment.

The use of vinylcyclohexane dioxide (VCD) as a polar dehydrant with subsequent embedment in Spurr was studied. The utilization of Epon 812 resin (E 812), hydroxyethyl methacrylate (HEM) and hydroxypropyl methacrylate (HPM) as polar dehydrants for Epon embedment were re-examined, and a polar Epon mix was introduced. The most effective dehydration sequence was: first 90%, then 95% VCD in water for 5 min. followed by two 20 min changes of 100% VCD. After 1 hr in equal quantities of VCD and Spurr mix, tissues were infiltrated with Spurr embedment (two 1 hr changes and overnight) and finally embedded in Spurr and polymerized at 60 degrees C for 16 hr. The most utilizable polar Epon mix was determined to be Epon 812 = 50 ml, NMA=42 ml, DMP-30=1-2 ml. It was somewhat brittle but cut well with both glass and diamond knives. All four polar dehydrants were found to retain lipids and carbohydrates equally well in thin section in striated and cardiac muscle, liver, kidney and brain from the rat. The E 812 was the only dehydrant that retained lung multilamellar bodies. The possible carcinogenic effects of VCD were considered and the probably metabolism and excretion of VCD were discussed.

Animals↗