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Biomedical subjects

C Jimenez

Publications and source records attributed to C Jimenez.

At least 91 records · Page 5Linked to original sources
PubMed1990

Amniotic fluid glucose concentration: a rapid and simple method for the detection of intraamniotic infection in preterm labor.

The purpose of this study was to determine whether amniotic fluid glucose concentrations is of value in the rapid diagnosis of intraamniotic infection. Amniocenteses were performed in 168 patients with preterm labor and intact membranes. Amniotic fluid was cultured for aerobic and anaerobic bacteria, as well as Mycoplasma species. The prevalence of positive amniotic fluid cultures was 13.6% (23/168). Patients with positive amniotic fluid cultures for microorganisms had significantly lower median amniotic fluid glucose concentrations than patients with negative amniotic fluid cultures (median 11 mg/dl, range 2 to 30 mg/dl vs median 28 mg/dl, range 3 to 74, respectively; p less than 0.001). Amniotic fluid glucose concentrations below 14 mg/dl had a sensitivity of 86.9% (20/23), a specificity of 91.7% (133/145), a positive predictive value of 62.5% (20/32), and a negative predictive value of 97.8% (133/136) in the detection of a positive amniotic fluid culture. Amniotic fluid glucose determination is a rapid, sensitive, inexpensive, and simple test for the detection of intraamniotic infection in women with preterm labor and intact membranes.

Amniotic Fluid↗
PubMed1990

Immunochemical localization and association with spermatozoa of androgen-regulated proteins of MR 24000 secreted by the mouse epididymis.

A polyclonal monospecific immune serum was raised against androgen-regulated proteins with Mr 24000 secreted by the mouse caput epididymidis. Sections of frozen tissues from the different regions of the epididymis have been studied by indirect immuno- fluorescence. Results indicate that the antigens are secretory proteins produced by the epithelial cells of the caput epididymidis, essentially in the medial and distal segments. Accumulation of the antigens was observed in the lumen of the caput and the corpus epididymal duct. Subsequently, their association with the sperm surface occurred and persisted down to the cauda epididymidis.

Animals↗
PubMed1990

Isolated angiitis of the central nervous system in childhood.

Isolated angiitis of the central nervous system, a rare inflammatory condition of the nervous system, characterized by vasculitis of the small vessels, has not, to the best of our knowledge, been described in childhood. It usually presents with a diversity of neurological symptoms in the fifth to eighth decades of life. We reviewed the case of an 8 year old male with autopsy proven isolated cerebral angiitis, to encourage the consideration of this disorder in children; and to emphasize the lack of sensitivity of present modalities of neurological investigation in the diagnosis.

Brain Diseases↗
PubMed1990

Molecular cloning of a cDNA for androgen-regulated proteins secreted by the mouse epididymis.

A cDNA clone encoding androgen-dependent proteins secreted by the mouse epididymis was isolated by screening a cDNA library using differential hybridization, according to the selective expression of the mRNA in the normal but not in the castrated mouse. Translation of mRNA hybrid-selected by this 1.4 kb clone (M53) yielded proteins of Mr 26,000 which were processed in vitro in the presence of microsomal membranes into proteins of Mr 24,000. Northern blot analysis of epididymal total RNA revealed at least two populations of mRNA (1.4 and 1.8 kb) homologous to the M53 clone, which were restricted to the caput epididymidis. Studies in vivo demonstrated that testosterone regulates the concentration of these mRNA populations. Analysis of epididymal total RNA from ten individual animals provided no evidence that the M53 mRNA populations are the products of allelic variants of a gene. Southern analysis of mouse genomic DNA revealed single bands with most of the tested restriction enzymes. Furthermore, cross-hybridization to the M53 cDNA revealed homologous mRNA species in rat, human, rabbit, ram and boar epididymal RNA.

Androgens↗
PubMed1989

Androgen-dependent messenger RNA(s) related to secretory proteins in the mouse epididymis.

Total RNA from mouse epididymides was translated in a cell-free system derived from rabbit reticulocyte lysate. The androgen dependence of a highly represented mRNA(s) was detected. This mRNA(s) encoded for a band of Mr 26,000 visualized by denaturing gel electrophoresis (SDS-PAGE). No other mouse sexual tissues (testis, vas deferens and seminal vesicle), liver, kidney or striated muscle presented such a band. Furthermore, this mRNA(s) was restricted to the caput epididymidis. Two-dimensional gel electrophoresis (2D-PAGE) showed that this band of Mr 26,000 was composed of 6 basic polypeptides. This translated protein may correspond to a newly synthesized secretory protein of Mr 24,000. The concentration of translatable mRNA(s) encoding for the band of Mr 26,000 dropped to 31% of the normal level at 3 days after castration and to 7%, 20 days later. Administration of testosterone to 30-day-castrated mice partly reversed these changes: 32% of the control value was reached after 10 days of treatment. Accumulation of this mRNA(s) was also just detectable (7% of the mature level) at 10 days of age. The high levels attained by 20 days of age (60% of the control value) suggest that the greatest increase of accumulation for this mRNA(s) occurs during the prepubertal period.

Animals↗
PubMed1989

A new method for demonstrating argyrophil cells of the pancreas and intestines.

A new method for demonstrating argyrophil cells of the pancreas and intestinal tract using a combined silver and reducing solution in sections of formaldehyde fixed tissue is described. Impregnating sections in a 60 C water bath, the procedure takes about 25 min. A microwave version that takes about 5 min is also given. Results are similar to those obtained with the Grimelius method for argyrophil cells.

Formaldehyde↗
PubMed1988

Plasmacytoma and gastric amyloidosis associated with nodular pulmonary amyloidosis.

The authors report an association of gastric and pulmonary amyloidosis in a 74 year old woman. The patient had had a gastric plasmacytoma for the last 11 years. On autopsy, the stomach was found to contain intramural deposits of amyloid, and was infiltrated by plasma cells secreting monoclonal IgG lambda light chain. In the lungs, the plasma cells in the vicinity of the amyloid deposits proved to be polytypic, suggesting that these deposits originated from circulating precursor proteins.

Amyloidosis↗
PubMed1988

[Generalized mucormycosis. Apropos of a case caused by Absidia corymbifera].

This is the case of a woman, 49 year old, suffering from acute lymphoblastic common leukemia, who died from respiratory and cardiac insufficiency 47 days after the onset of aplasia. Autopsy revealed cardiac and cerebral dissemination of pulmonary mycosis. Absidia corymbifera (Mucoraceae, Phycomycete) was shown by culture to be the pathogen involved.

Female↗
PubMed1987

Occurrence of immunoblastic B-cell lymphoma in hairy cell leukemia.

A 47-year-old man, was referred for evaluation of asymptomatic splenomegaly in September 1981, and a diagnosis of hairy cell leukemia (HCL) at the initial clinical stage was made. The patient remained asymptomatic until May 1985, when splenectomy was performed because of anemia and splenomegaly. Bone marrow and liver biopsy specimens showed diffuse infiltration by abnormal tartase resistant acid phosphatase (TRAP) positive lymphocytes with typical aspect of hairy cells. Four months later, he developed fever of unknown origin and, at laparotomy, diffuse retroperitoneal lymph node enlargement and metastatic liver nodules were seen. Lymph node and liver biopsy specimens showed diffuse infiltration by abnormal large lymphocytes, which bore monoclonal surface immunoglobulin M and light chain kappa. Only six cases of non-Hodgkin's lymphoma associated with HCL have been published to date. This report describes an additional case of immunoblastic B-cell lymphoma, preceded 4 years earlier by the diagnosis of HCL.

B-Lymphocytes↗
PubMed1987

A combined elastic, fibrin and collagen stain.

A method is described which demonstrates nuclei, elastic fibers, red blood cells, collagen and fibrin. Nuclei and elastic fibers are stained by a modified Verhoeff's elastic tissue stain which was previously developed and used in the elastic-Masson combination. Both early fibrin and red blood cells are shown by lissamine fast yellow. Mature fibrin, some types of collagen and other cytoplasmic changes are stained by a combination of acid fuchsin, Biebrich scarlet and ponceau 2R, while old fibrin is demonstrated by the collagen stain. This method takes about 1 hr to perform and has the added advantage that several entities are clearly shown in a single slide.

Cell Nucleus↗
PubMed1987

Incidence and clinical significance of peripheral and bone marrow basophilia.

We reviewed 2110 bone marrow aspirations from the same number of patients to establish the incidence and associations of peripheral and bone marrow basophilia. Of these, 125 cases of marrow basophilia (5.9% incidence) and 63 cases of peripheral basophilia (3.0% incidence) were identified. There were 33 patients with simultaneous marrow and peripheral basophilia, which was only significantly associated with chronic myelogenous leukemia (24 cases). Isolated peripheral basophilia was rarely seen (30 patients, 1.4% incidence) and it did not reflect any significant pathologic association. Marrow basophilia was significantly present in chronic myeloproliferative disorders, idiopathic myelodysplasia, certain erythrocyte disorders, such as iron deficiency anemia, and aplastic anemia. The incidence of marrow basophilia in patients with lymphoma, acute leukemia, or solid carcinoma was not significantly different from what it would be as a chance occurrence. Our findings suggest that marrow basophilia is a specific, but not sensitive, marker of myeloproliferative and dysmyelopoietic syndromes.

Adult↗
PubMed1986

Improving results from nerve and muscle biopsy in children: technical considerations in the harvesting of specimens.

The convergence of expertise in the neurological sciences, special interest in myoneuropathology, and improved surgical technique has resulted in a significantly improved yield from muscle and nerve biopsies in children with suspected neuromuscular disorders. We present a synthesis of all those factors that we have identified as contributing to optimal results, particularly site selection, sample size, a meticulous biopsy technique that avoids the introduction of artefacts, the use of the muscle biopsy clamp, prompt processing of the specimen, and participation of the neurologist in the biopsy procedure and the interpretation of the histologic sections.

Biopsy↗
PubMed1986

Infantile mammary duct ectasia: a cause of bloody nipple discharge.

Bloody nipple discharge in infancy has been rarely reported in the medical literature. Its cause is unknown. We report a three-year-old male infant and a five-month-old female infant with bloody nipple discharge. Because of persistent bloody discharge, a subcutaneous mastectomy was performed in the boy; the problem resolved in the girl after a period of observation. The specimen showed histologic changes identical to those seen in adult mammary duct ectasia. All the endocrinologic work-up was normal. We suspect that bloody nipple discharge in infancy is underreported. This is a benign condition with histologic changes similar to adult mammary duct ectasia and if persistent, should be properly investigated; biopsy or excision are not indicated.

Blood↗