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C Joyce

Publications and source records attributed to C Joyce.

41 records · Page 3Linked to original sources
PubMedDate not supplied

Release, extraction, and stability of hyaluronidase associated with human spermatozoa. Comparisons with the rabbit.

In contrast to the rabbit: 1) no hyaluronidase could be detected in fresh or frozen human seminal plasma, all color formation on assay being due to a dialyzable, heat stable factor; 2) almost no hyaluronidase could be extracted from frozen-thawed human testicles; 3) the hyaluronidase from human spermatozoa was rapidly inactivated upon release, either spontaneously or on extraction; and 4) a large decrease in hyaluronidase activity occurred when human spermatozoa were stored under various conditions. Rabbit spermatozoa contained six to 13 times more hyaluronidase than human spermatozoa. These results show that distinct species differences exist in the hyaluronidase associated with spermatozoa. None of the human genital tract sources studied could be used to obtain adequate amounts of hyaluronidase for the further isolation and purification of the enzyme. For both human and rabbit spermatozoa, it was optimal to add the cells directly to the assay system for the quantitation of hyaluronidase on spermatozoa rather than extracting the spermatozoa and testing the extracts. The spermatozoa of both species appear to be capable of digesting hyaluronic acid directly. As had previously been found for acrosin, a higher amount of hyaluronidase was maintained when human spermatozoa were cryopreserved in a zwitter buffer (TESTCY) rather than in glycerol.

Animals↗
PubMedDate not supplied

Applications of the polymerase chain reaction (PCR) in diagnosis.

This paper reports on three applications of the polymerase chain reaction (PCR) in the pathology laboratory, cystic fibrosis carrier status investigation, Staphylococcus aureus identification and HLA-DQ alpha tissue typing. Allele specific PCR was used to detect the common cystic fibrosis mutation (delta F508) in an Irish family. The genomic status of each member was shown to be either heterozygous (carrier) or homozygous (affected) for the mutation, based on the resolution of allelic amplifications in an agarose gel. Five staphylococci were subjected to PCR designed to amplify a 137 bp DNA fragment from the S. aureus protein A gene (spa). Four isolates gave a coloured "dot-blot" positive signal, the fifth was negative. In the final application the HLA-DQ alpha region was amplified using DNA obtained from four unrelated individuals. Amplified DNA was tissue typed in a reverse "dot-blot" format. All individuals had unique HLA-DQ alpha types. These examples have been chosen to demonstrate the versatility of this technique and to illustrate some of its potential applications.

Alleles↗
PubMedDate not supplied

Utilization of somatosensory evoked cortical potentials in spinal cord injury. Prognostic limitations.

The prognostic value of somatosensory evoked cortical potentials (SECP) for clinical recovery was studied in 71 patients with complete (28) and incomplete (43) spinal injuries. While the absence of an SECP was associated with no clinical recovery, the presence of an SECP was of little value in predicting the clinical state at the time of examination or the potential for recovery.

Adolescent↗