Biomedical subjects

C Juby

Publications and source records attributed to C Juby.

2 recordsLinked to original sources

PubMed1994

Specific targeting of antisense oligonucleotides to neutrophils.

The ability of three different hydrophobic ligands (cholic acid, cholesterol, and the tetrapeptide fMLFY) to increase the uptake of an antisense (anti-actin) oligomer into neutrophils was analyzed. In agreement with the literature (Boutorin et al., 1989; Letsinger et al., 1989), we found that cholic acid and cholesterol conjugates greatly enhance the uptake of anti-actin oligomer. When fMLFY is the ligand, the cellular uptake is much less than that of anti-actin oligomer alone, but the biological consequences are much more significant. Our results are consistent with the hypothesis that the fMLFY conjugate of the anti-actin oligomer is internalized via a different route, and reaches its target site most efficiently.

Actins↗

PubMed1988

A high capacity, reusable oligodeoxythymidine affinity column.

A high capacity, reusable oligodeoxythymidine (oligo(dT)) affinity column suitable for purification of polyadenylated mRNA is described. Oligo(dT) molecules, 30 nucleotides in length, were chemically synthesized on an Applied Biosystems DNA synthesizer. Deprotection of the oligo(dT) molecules was achieved by thiophenol/dioxane/triethylamine treatment. Since no hot ammonia treatment was required, the oligo(dT) molecules remained covalently attached to the support matrix. The resulting column has very high oligo(dT) density and can be used directly for purifying polyadenylated mRNA. Column-purified mRNAs were shown to be intact and functional in Northern blot analysis, in vitro translation, and reverse transcription assays.

Animals↗