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Biomedical subjects

C Juste

Publications and source records attributed to C Juste.

31 records · Page 2Linked to original sources
PubMed1986

Enterohepatic circulation of bile acids in pigs: diurnal pattern and effect of a reentrant biliary fistula.

The present studies were undertaken 1) to assess the quantitative absorption of total bile acids in the portal vein of normally fed, unanesthetized pigs with an intact biliary tract and 2) to evaluate the effect of a chronic reentrant biliary fistula on absorption. In the intact biliary tract group (5 pigs), mean total bile acid concentration averaged 83.9 +/- 2.0 and 15.6 +/- 0.6 microM in portal and arterial blood, respectively, and 216.9 +/- 20.4 mmol of total bile acids were absorbed over 24 h. Time courses of blood concentration and absorption were described all along the light-dark cycle, and hepatic uptake averaged 79.3 +/- 0.5%. A reentrant choledocal fistula in the upper duodenum (5 pigs) did not affect mean daily blood concentrations (87.5 +/- 2.4 and 19.1 +/- 0.8 microM in portal and arterial blood, respectively), daily absorption (226.4 +/- 23.7 mmol, i.e., 93.5% of the daily secretion), or hepatic uptake (76.4 +/- 1.1%), but it did seem to influence the daily kinetics of blood concentration and absorption. These studies supply quantitative and kinetic data about the absorptive stage of the enterohepatic cycle in pigs and show that a bile fistula is suitable for the study of biliary dynamics.

Animals↗
PubMed1983

Bile secretion in the fistulated pig: effect of the method used for bile reinfusion.

The aim of this work was to investigate the effects on bile secretion of flow rate and site of reinfusion of the collected bile to the animal. Thirty-two pigs weighing 50 +/- 3 kg at the beginning of the experiment were fitted with a reentrant fistula in the lower common bile duct and in the upper duodenum. Bile collected from the bile duct was reinfused in four different ways (four groups of 8 animals each): into the duodenum or the lower common bile duct at a constant flow rate using a peristaltic pump, or into the duodenum or the lower common bile duct at a rate mimicking the flow rate of the secretion using an automatic apparatus. Reinfusing the bile into the lower common bile duct at a rate mimicking the secretion rate provided a daily bile acid production about 21% higher than the level recorded with the other three methods. This was mainly due to a higher bile acid concentration since the bile flow was only slightly affected by the treatment.

Animals↗
PubMed1983

By-passing the sphincter of Oddi does not affect gallbladder emptying in the pig.

A study of the relationship between bile secretion and nutrition in the pig requires a complete and continuous collection of the bile and its reinfusion to the animal. In most of the studies performed in different species, bile has been directly reinfused into the duodenum, leading to the exclusion of the sphincter of Oddi from the biliary pathway. It has been postulated that such an exclusion could inhibit gallbladder emptying. The aim of the present work was to study postprandial gallbladder emptying in the pig, depending on the site of bile reinfusion, i.e. the duodenum or the lower bile duct. The gallbladder bile was coloured with indocyanine green (ICG) and marker secretion was recorded after a test-meal. The results showed that after meal intake, the gallbladder emptied over a similar period of time and according to similar kinetics, whatever the site of bile reinfusion.

Ampulla of Vater↗
PubMed1983

Response of bile flow, biliary lipids and bile acid pool in the pig to quantitative variations in dietary fat.

Bile flow, biliary lipids and bile acid pool have been studied in growing pigs adapted to a semipurified diet of 2, 10 or 20% lard. After a 5-day period of apparent digestibility measurement, bile secretion was studied in previously fistulated pigs. The apparent digestibility of lard was maintained at a very high level, whatever quantity of lard was ingested. Daily bile acid output was 49% higher with the 10% lard diet than with the 2% lard diet, whereas no further increase was observed with the 20% lard diet. There was a direct relationship between bile acid output and pool size. Biliary phospholipid output increased by 29.0% between 2 and 10% dietary lard and by 69.5% between 10 and 20% lard. The corresponding rise in biliary cholesterol output was +33% between 2 and 10 or 10 and 20% lard in the diet. A study of biliary kinetics showed that this adaptation of biliary lipid to dietary fat was observed whatever time of the light/dark cycle was considered. These results, together with a recent demonstration of the implication of endogenous phospholipids in fat digestion, suggested that, in the course of biliary lipid adaptation to dietary fat content, biliary phospholipid could act in synergy with bile salt, resulting in the maintenance of excellent dietary fat digestion.

Animals↗
PubMed1983

Bile restitution procedures for studying bile secretion in fistulated pigs.

In order to study bile secretion in the fistulated pig, an apparatus which reinfused the secreted bile at a rate reflecting the secretion rate, was used. The essential parts were a glass container equipped with two platinum wire electrodes, an electronic relay, a stop valve, and a peristaltic pump. This apparatus allowed a reinfusion of collected bile in a volume determined by the position of one of the electrodes, the recording of bile flow, and a continuous sampling for chemical analysis.

Animals↗
PubMed1982

[Diversion of bile into the distal ileum in swine: effect on bile acid secretion and digestive utilization of the diet].

Bile salt output and digestive utilization of a high-lipid diet were studied in pig bile collected from the common bile duct and infused into the duodenum then into the distal ileum of the animal. Five Large-White pigs of 50 kg body-weight were fitted with three permanent fistulae, one in the common bile duct for complete collection of bile secretion, and the other two in the duodenum and the ileum (at about 80 cm from the ileo-caecal valve) for returning the secretion to the animal. The diversion of the bile into the distal ileum led to a significant 82 p. 100 decrease in daily bile acid output and a 36 p. 100 decrease in apparent digestibility of dietary energy when compared with values obtained when bile was infused into the duodenum. The latter decrease was mainly due to a marked diminution ( - 60.4 p. 100) of the digestive utilization of the dietary lipids. The results show the role of the pig small intestine in maintaining an efficient enterohepatic circulation of bile acid as a result of optimal utilization of the dietary lipids.

Animals↗
PubMed1981

Digestive enzymes in the germ-free animal.

The digestive physiology of the germ-free animal has a number of characteristics (cecal hypertrophy, slower small intestine cell renewal, slower gastric emptying and intestinal transit) which distinguish it from that of the conventional animal. If the germ-free model is to be used to determine the role of gastrointestinal microflora in the nutrition of the conventional animal, it is essential to complete the study of these characteristics by data on digestive enzymes in the germ-free. The present paper analyzes these data. There is little information on salivary amylase and none on gastric proteolytic enzymes and intestinal peptidases. More complete data on exocrine pancreas enzymes and intestinal disaccharidases show that the digestive equipment is similar in germ-free and conventional animals. Bile salts, not considered as digestive enzymes, are qualitatively and quantitatively different, depending on the digestive tract bacterial environment. In general, the germ-free animal has some characteristics which should permit better utilization of the diet ingested. Measurements of apparent digestibility do not confirm this hypothesis since results obtained in germ-free and conventional animals of the same species are contradictory.

Animals↗
PubMed1980

[Treatment of acute brucellosis with cotrimoxazole, doxicyclin and streptomycin. A comparative study (author's transl)].

Human brucellosis can be managed with different therapeutic measures. The present study compares the therapeutic efficacy of the classical treatment with streptomycin, doxicyclin and cotrimoxazole, every one of the treatment regimes being applied to 19 patients with acute brucellosis. The results do not demonstrate an advantage of the new association over the classical treatment. Relapses, time elapsed until the patient was afebrile and drug tolerance were similar for both treatments. The classical treatment has a lower cost but the new association implies more patient comfort because the number of pills to be ingested daily is reduced to less than half, a fact that could make it the treatment of choice.

Acute Disease↗
PubMedDate not supplied

Effects of soy protein diet on digestive lumenal polyamines and colonic cell proliferation in pigs.

This study was performed to determine whether erythrocyte and digestive lumenal polyamine concentrations are affected by a soy protein diet when compared to a casein diet. We also determined the effects of these diets on colonic cell proliferation. Sixteen pigs received either a 16% soy protein or casein diet for 25 days. The erythrocyte putrescine was higher in pigs fed the soy protein diet. Significant levels of polyamines were observed in the digestive lumen on both diets. Lumenal putrescine and cadaverine were higher in the proximal colon in the casein group. Lumenal spermidine was higher in the caecum and colon in the soy protein group. No significant differences in the ornithine decarboxylase activity nor in the proliferative cell nuclear antigen labelling index were observed in the colonic mucosa regardless of the regimen. These results indicate that the dietary source of protein induces significant changes in lumenal polyamines in the colon. The physiological effects of these changes need to be further investigated.

Animals↗