PubMed Health⌕ Search

Biomedical subjects

C K Ching

Publications and source records attributed to C K Ching.

66 records · Page 4Linked to original sources
PubMed1988

Identification and partial characterization of a new pancreatic cancer-related serum glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and lectin blotting.

The combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peanut agglutinin lectin blotting has been used to identify a pancreatic cancer-related glycoprotein in serum. Its high molecular weight and its lectin-binding characteristics suggest that it probably is a mucin. Comparison with immunoblotting with Ca 19-9 antibody suggests that the epitopes for Ca 19-9 and peanut agglutinin may be variably expressed on different sites of the same mucin. The peanut agglutinin-binding glycoprotein was demonstrated in 12 of 34 pancreatic cancer sera, including four with normal Ca 19-9 assay, and none of 96 control sera, including sera from patients with obstructive jaundice and other cancers. These preliminary results suggest that it may become a useful addition to the current serum markers for pancreatic cancer. The technique of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and lectin blotting may well prove valuable in the search for other cancer-related glycoprotein markers.

Antigens, Neoplasm↗
PubMed1988

Use of lectin histochemistry in pancreatic cancer.

Lectin peroxidase histochemical analysis was carried out on pancreatic tissue from patients with pancreatic carcinoma and chronic pancreatitis and from subjects with normal pancreas to find a tumour specific pattern of lectin binding that would aid histological and cytological diagnosis. There were striking differences between the lectin binding characteristics of the different cell types in the normal pancreas. Acinar cells were uniformly positive for binding with wheat germ agglutinin and soy bean agglutinin while islet cells were usually negative for these lectins. Ulex europaeus I lectin however, was found not to be specific for endothelium, showing positivity also for acinar and ductal tissue. Griffonia simplicifolia II lectin was found to be highly specific for ductal epithelium, and because of this was tested in a hamster pancreatic cancer model where it was not specific for ductal epithelium, reflecting differing carbohydrate expression in the hamster pancreas. Pancreatic carcinomas and chronic pancreatitis bound all five lectins without any qualitative distinction from each other or from normal pancreatic tissue, but there was increased intensity of peanut agglutinin binding to secreted mucins in pancreatic carcinoma, which may be of potential use in radiolabelled lectin scanning.

Animals↗
PubMedDate not supplied

Gastric cytoprotection by amoxycillin in the rat.

Amoxycillin in combination with anti-ulcer agents has been shown to prolong duodenal ulcer remission. While this effect can be related to the eradication of Helicobacter pylori, it is not known if amoxycillin might possess cytoprotective properties. Protection against ethanol-induced gastric mucosal damage by intragastric instillation of amoxycillin suspension (prepared from capsule form) and solution (from injection form, 100% pure amoxycillin), and by intraperitoneal injection of amoxycillin solution was studied using an ex vivo gastric chamber in the rat. Intragastric and intraperitoneal administration of amoxycillin dose-dependently protected the rat gastric mucosa from damage by absolute ethanol. This protection was lost when the rats were pretreated with indomethacin. Gastric mucosal blood flow as measured by laser Doppler flowmetry and gastric acid output were unaffected by amoxycillin. Amoxycillin imparts gastric cytoprotection, and one possible mechanism is by the release of prostaglandins.

Amoxicillin↗
PubMedDate not supplied

Drug therapy of peptic ulcer disease.

Healing of peptic ulcers can be achieved by using a variety of anti-ulcer medications. The most commonly used agents include the histamine-2 receptor antagonists (H2RAs) and the proton pump inhibitors. They are also efficacious in preventing ulcer recurrence providing maintenance treatment is given. The ideal treatment for peptic ulcers today is aiming at eradication of Helicobacter pylori infection. Successful elimination of the latter not only heals the ulcers but also provides a cure for the disease, so that the patients will no longer require lifelong maintenance medical therapy.

2-Pyridinylmethylsulfinylbenzimidazoles↗