Biomedical subjects

C Kennes

Publications and source records attributed to C Kennes.

At least 19 recordsLinked to original sources

PubMed2006

Combined post-ozonation and biological treatment of recalcitrant wastewater from a resin-producing factory.

In this study, effluent from the biological treatment of wastewater from a resin-producing factory containing recalcitrant compounds was ozonated under different conditions. Afterwards, the biodegradability of the ozonated effluent was studied under anoxic conditions. The post-ozonation of the industrial effluent was performed using a wide range of ozone doses, from 1.8 to 29.5 mg L(-1)min(-1). After the biological treatment of the ozonated effluent, organic carbon and nitrogen removals from 27 to 97% and from 27 to 80%, respectively, were achieved. The effect of the contact time was studied at a constant ozone dose of 13.0+/-1.2 mg L(-1)min(-1) and contact times ranging from 30 to 180 min. In this case, organic carbon removals from 55 to 100% and organic nitrogen removals from 41 to 77% were obtained after biological treatment.

Biodegradation, Environmental↗

PubMed2006

Effect of key parameters on the removal of formaldehyde and methanol in gas-phase biotrickling filters.

The effect of some important operation parameters, as pH, pollutant load and composition of the nutrient media, on the biodegradation of a mixture of formaldehyde and methanol in a gas-phase biotrickling filter was studied. pH proved to affect the degradation of both compounds at moderately acidic values. Replacing ammonium with nitrate as nitrogen source in the liquid solution led to a slight decrease in performance, though this difference was not really significant. A slight decrease in the elimination rate was also observed when reducing the N-NO(3)(-) concentration to 60% of its original value. No interactions between the two pollutants were found under our working conditions.

Bioreactors↗

PubMed2006

Development of a novel monolith-bioreactor for the treatment of VOC-polluted air.

A novel bioreactor packed with ceramic monolith colonized by a toluene-degrading culture was investigated in order to assess its suitability for waste gas treatment. Operational parameters that were considered included start-up of the bioreactor, toluene loading rate, changes in gas flow rate, liquid feed mode, and steady-state operation. This is the first report on the treatment of toluene-polluted air in such a biological monolith reactor. Data on performance and stability have been obtained showing that this system is suitable for waste gas treatment. Removal efficiencies around 90% could be maintained at different gas flow rates, although this value gradually dropped when increasing the load above 30 g m(-3) h(-1). Interestingly, omitting the continuous feed of a liquid trickling phase improved the reactor's performance. One potential drawback that needs to be minimized is related to clogging after long term operation. Further studies aimed at optimizing this novel application would allow reaching a high performance under long-term, stable conditions.

Air Pollutants↗

PubMed2005

Simultaneous nitrification and formaldehyde biodegradation in an activated sludge unit.

The simultaneous removal of formaldehyde and ammonium in a lab-scale activated sludge unit was investigated. The unit was operated at a hydraulic retention time of 2.4 days with an ammonium concentration in the influent of 350 mg NH4+-N/L, maintaining the ammonium loading rate at 0.15 g NH4+-N/Ld during the operation time. However, the applied organic loading rate was increased stepwise by increasing the formaldehyde concentration from 26 up to 3168 mg/L, corresponding to 0.01-1.40 g COD/Ld. High formaldehyde removal efficiencies, around 99.5% (+/-0.38), were maintained at all the formaldehyde concentrations. Ammonium removal was also very high during the operation period, around 99.9% (+/-0.01). The ammonium concentration in the effluent was lower than 0.1 mg NH4+-N/L at all applied organic loading rates, indicating that there was no inhibition of nitrification by formaldehyde.

Ammonia↗

PubMed2005

Phenol biodegradation and its effect on the nitrification process.

Phenol biodegradation under aerobic conditions and its effect on the nitrification process were studied, first in batch assays and then in an activated sludge reactor. In batch assays, phenol was completely biodegraded at concentrations ranging from 100 to 2500 mg l(-1). Phenol was inhibitory to the nitrification process, showing more inhibition at higher initial phenol concentrations. At initial phenol concentrations above 1000 mg l(-1), the level of nitrification decreased. In the activated sludge reactor, the applied ammonium loading rate was maintained at 140 mg N-NH(4)(+)l(-1)d(-1) (350 mg N-NH(4)(+)l(-1)) during the operation time. However, the applied organic loading rate was increased stepwise from 30 to 2700 mg COD l(-1)d(-1) by increasing the phenol concentration from 35 up to 2800 mg l(-1). High phenol removal efficiencies, above 99.9%, were maintained at all the applied organic loading rates. Ammonium removal was also very high during the operation period, around 99.8%, indicating that there was no inhibition of nitrification by phenol.

Bacteria, Aerobic↗

PubMed2005

Partial nitrification of wastewater from an aminoplastic resin producing factory.

Nitrification via nitrite was studied in two aerobic reactors treating wastewater from an aminoplastic resin producing factory at HRT varying between 1.37-1.89 and 2.45-3.63 days. Both eactors were fed with concentrations of 366, 450, 1099 and 1899 mg N-NH4+/L. In general in the reactor operated at a lower HRT, the nitritation percentage decreased from 87.2 to 21.6%, while the nitratation percentage remained always lower than 2.5% (except in the last period) when the ammonium concentration was increased. This behaviour could be due to the inhibition of the ammonium and nitrite oxidation produced by high free ammonia concentrations up to 179.3 mg N-NH3/L. In the reactor operated at a higher HRT, the nitritation percentage decreased and the nitratation percentage increased from 88.6 to 39.6% and from 0.65 to 35.7%, respectively, due to an increase of the dissolved oxygen concentration from 0.76 to 1.02 mg O2/L. However, when ammonium was fed at a concentration of 1898.7 mg N-NH4+/L, the nitritation increased and the nitratation decreased, probably as a result of the accumulation of free ammonia up to 2.04 mg N-NH3/L, meaning that nitrite oxidizers were inhibited. Nitrite build-up was observed after each modification of ammonium concentration in the feed.

Aerobiosis↗

PubMed2004

Biodegradation and effect of formaldehyde and phenol on the denitrification process.

Formaldehyde and phenol biodegradation during the denitrification process was studied at lab-scale, first in anoxic batch assays and then in a continuous anoxic reactor. The biodegradation of formaldehyde (260 mgl(-1)) as single carbon source and at phenol concentrations ranging from 30 to 580 mgl(-1) was investigated in batch assays, obtaining an initial biodegradation rate around 0.5g CH(2)OgVSS(-1)d(-1). With regard to phenol, its complete biodegradation was only observed at initial concentrations of 30 and 180 mgl(-1). The denitrification process was inhibited at phenol concentrations higher than 360 mgl(-1). Studies were also done using a continuous anoxic upflow sludge blanket reactor in which formaldehyde removal efficiencies above 99.5% were obtained at all the applied formaldehyde loading rates, between 0.89 and 0.14g COD (CH(2)O)l(-1)d(-1). The phenol loading rate was increased from 0.03 to 1.3g COD (C(6)H(6)O)l(-1)d(-1). Phenol removal efficiencies above 90.6% were obtained at phenol concentrations in the influent between 27 and 755 mgl(-1). However, when the phenol concentration was increased to 1010 mgl(-1), its removal efficiency decreased. Denitrification percentages around 98.4% were obtained with phenol concentrations in the influent up to 755 mgl(-1). After increasing phenol concentration to 1010 mgl(-1), the denitrification percentage decreased because of the inhibition caused by phenol.

Biodegradation, Environmental↗

PubMed2004

Hydrodynamic behaviour and comparison of technologies for the removal of excess biomass in gas-phase biofilters.

The hydrodynamic behaviour of a biofilter fed toluene and packed with an inert carrier was evaluated on start-up and after long-term operation, using both methane and styrene as tracers in Residence Time Distribution experiments. Results indicated some deviation from ideal plug flow behaviour after 2-year operation. It was also observed that the retention time of VOCs gradually increased with time and was significantly longer than the average residence time of the bulk gas phase. Non-ideal hydrodynamic behaviour in packed beds may be due to excess biomass accumulation and affects both reactor modeling and performance. Therefore, several methods were studied for the removal of biomass after long-term biofilter operation: filling with water and draining, backwashing, and air sparging. Several flow rates and temperatures (20-60 degrees C) were applied using either water or different chemicals (NaOH, NaOCl, HTAB) in aqueous solution. Usually, higher flow rates and higher temperatures allowed the removal of more biomass, but the efficiency of biomass removal was highly dependent on the pressure drop reached before the treatment. The filling/draining method was the least efficient for biomass removal, although the treatment did basically not generate any biological inhibition. The efficiency of backwashing and air sparging was relatively similar and was more effective when adding chemicals. However, treatments with chemicals resulted in a significant decrease of the biofilter's performance immediately after applying the treatment, needing periods of several days to recover the original performance. The effect of manually mixing the packing material was also evaluated in duplicate experiments. Quite large amounts of biomass were removed but disruption of the filter bed was observed. Batch assays were performed simultaneously in order to support and quantify the observed inhibitory effects of the different chemicals and temperatures used during the treatments.

Biodegradation, Environmental↗

PubMed2004

Formaldehyde and urea removal in a denitrifying granular sludge blanket reactor.

Simultaneous formaldehyde biodegradation, urea hydrolysis and denitrification in anoxic batch assays and in a continuous laboratory anoxic reactor were investigated. In batch assays, the initial formaldehyde biodegradation rate was around 0.7 g CH(2)Og VSS(-1)d(-1) and independent of the urea concentration (90- 370 mg N-NH(2)CONH(2)l(-1)). Urea was completely hydrolyzed to ammonium in the presence of 430 mg l(-1) formaldehyde and complete denitrification took place in all cases (125 mg N-NO(-)(3)l(-1)). Formaldehyde removal efficiencies above 99.5% were obtained in a lab-scale denitrifying upflow sludge blanket reactor at organic loading rates between 0.37 and 2.96 kg CODm(-3)d(-1) (625-5000 mg CH(2)Ol(-1)). The urea loading rate was increased from 0.06 to 0.44 kg Nm(-3)d(-1) (100-800 mg N-NH(2)CONH(2)l(-1)) and hydrolysis to ammonium was around 77.5% at all loading rates. The denitrification process was always almost complete (100-800 mg N-NO(3)(-)l(-1)), due to the high COD/N ratio of 6.7 in the influent. A minimum value of 3.5 was found to be required for full denitrification. The composition of the biogas indicated that denitrification and methanogenesis occurred simultaneously in the same unit. A good granulation of the sludge was observed.

Biodegradation, Environmental↗

PubMed2002

Optimization of nutrient supply in a downflow gas-phase biofilter packed with an inert carrier.

Several methodologies were tested to supply nutrients to a downflow biofilter packed with perlite and used to treat toluene-polluted air. Despite the presence of an inorganic carrier, elimination capacities of up to around 60 g/m(3) per hour could be maintained when a basal medium, containing nitrogen, phosphorus and potassium, was supplied once every fortnight or even once a month rather than once a week. Experimental results also indicated that the addition of vitamins or trace minerals to the basal aqueous medium hardly improved biofilter performance. Furthermore, the nutrient supply could be combined with a biomass control strategy, using air sparging, without any adverse effect on biofilter performance compared to supplying nutrients alone, and limiting the accumulation of excess biomass on the packing material. The performance of the biofilter was not significantly affected by temperature fluctuations between 25 and 33 degrees C.

Air Pollutants↗

PubMed2001

Parameters affecting performance and modeling of biofilters treating alkylbenzene-polluted air.

Both short-term and long-term biofiltration experiments were undertaken with a biofilter inoculated with a defined microbial consortium and treating an alkylbenzene mixture. The results obtained with such a biofilter in short-term experiments were very similar to those obtained with a biofilter inoculated with a non-defined mixed culture, in terms of maximum elimination capacities (70-72 g m(-3) h(-1)) and the corresponding removal efficiencies (>95%). However, in long-term experiments, a better performance was reached, with a maximum elimination capacity of 120 g m(-3) h(-1), corresponding to a removal efficiency >99% after 2 years of operation. Inoculation proved to be useful for shortening the start-up period. In the long term, it appeared that biomass distribution was not homogenous along the biofilter, which in some cases resulted in a bad fit between simple model equations and experimental data.

Air Pollutants↗

PubMed2001

Kinetics of inhibition in the biodegradation of monoaromatic hydrocarbons in presence of heavy metals.

The toxicity and inhibitory effects of heavy metals such as cadmium, nickel and zinc on alkylbenzene removal were evaluated with a Bacillus strain. The kinetics of alkylbenzene biodegradation with the different heavy metals at various concentrations were modeled using the Andrews equation which yielded a good fit between model and experimental data. Additional experiments undertaken with a Pseudomonas sp. in presence of nickel confirmed a good fit between experimental data and the Andrews model for this strain as well. The heavy metals inhibition constants (Ki) were calculated for different combinations of volatile organic compounds (VOC) and heavy metals. The present approach provides a method for evaluating and quantifying the inhibition effect of heavy metals on the biodegradtion of pollutants by specific microbial strains.

Bacillus↗

PubMed2001

Start-up and long-term performance of a gas phase biofilter packed with an inert carrier.

Several parameters affecting the performance and characteristics of alkylbenzene fed biofilters were checked when using perlite as inert carrier material. The influence of the inoculum on startup and long term performance was studied using both non defined as well as defined cultures. The inoculated pure cultures, which were still detected in the biofilter after several months operation, allowed shortening the start-up period Qualitative and quantitative biomass distribution appeared to be uneven along the biofilter bed in long term operation studies. The biofilter could withstand a drying period of several weeks although such operating condition led to a shift in dominant microbial populations in the biofilm which was followed by an increase of the biofilter performance. Although a water content of 55-60% appeared to be optimal with perlite, peformance data were still satisfactory down to a water content around 35-40%.

Benzene Derivatives↗

PubMed1999

Biofilter performance and characterization of a biocatalyst degrading alkylbenzene gases.

A biofilter treating alkylbenzene vapors was characterized for its optimal running conditions and kinetic parameters. Kinetics of the continuous biofilter were compared to batch kinetic data obtained with biofilm samples as well as with defined microbial consortia and with pure culture isolates from the biofilter. Both bacteria and fungi were present in the bioreactor. Five strains were isolated. Two bacteria, Bacillus and Pseudomonas, were shown to be dominant, as well as a Trichosporon strain which could, however, hardly grow on alkylbenzenes in pure culture. The remaining two strains were most often overgrown by the other three organisms in liquid phase batch cultures. mu max, KS, KI values and biodegradation rates were calculated and compared for the different mixed and pure cultures. Since filter bed acidification was observed during biofiltration studies reaching a pH of about 4, experiments were also undertaken to study the influence of pH on performance of the different cultures. Biodegradation and growth were possible in all cases, over the pH range 3.5-7.0 at appreciable rates, both with mixed cultures and with pure bacterial cultures. Under certain conditions, microbial activity was even observed in the presence of alkylbenzenes down to pH 2.5 with mixed cultures, which is quite unusual and explains the ability of the present biocatalyst to remove alkylbenzenes with high efficiency in biofilters under acidic conditions.

Bacillus↗

PubMed1998

Methanogenic and perchloroethylene-dechlorinating activity of anaerobic granular sludge.

The biodegradation and toxicity of tetrachlorethylene (C2Cl4) and trichloroethylene (C2HCl3) were studied with different anaerobic enrichment cultures using the following electron donors: acetate, propionate, butyrate, methanol, formate and hydrogen. All of them sustained dechlorination except propionate, for which C2Cl4 biodegradation rates were not significant. The best results were obtained with butyrate. Hydrogen appeared to be a relevant electron donor for dechlorination with the present cultures. In the presence of specific inhibitors such as bromoethanesulphonate or molybdate, a slight inhibition of dechlorination was observed. According to dechlorination kinetics, Monod-type behaviour was observed up to 120 microM C2Cl4 or 200 microM C2HCl3 with Ks values around 7 microM for both compounds. Dechlorination was partially inhibited at higher concentrations. In contrast, methanogens, or at least methane production, were more sensitive to the presence of chlorinated ethylenes and inhibitions of methanogenesis was observed to different extents over all the C2Cl4/C2HCl3 concentration range tested, even at the lowest concentrations.

Acetates↗

PubMed1996

Anaerobic dechlorination and mineralization of pentachlorophenol and 2,4,6-trichlorophenol by methanogenic pentachlorophenol-degrading granules.

Anaerobic granules developed for the treatment of pentachlorophenol (PCP) completely mineralized 14C-labeled PCP to 14CH4 and 14CO2. Release of chloride ions from PCP was performed by live cells in the granules under anaerobic conditions. No chloride ions were released under aerobic conditions or by autoclaved cells. Addition of sulfate enhanced the initial chloride release rate and accelerated the process of mineralization of 14C-labeled PCP. Addition of molybdate (10 mM) inhibited the chloride release rate and severely inhibited PCP mineralization. This suggests involvement of sulfate-reducing bacteria in PCP dechlorination and mineralization. Addition of 2-bromoethane sulfonate slightly decreased the chloride release rate and completely stopped production of 14CH4 and 14CO2 from [14C]PCP. 2,4,6-trichlorophenol was observed as an intermediate during PCP dechlorination. On the basis of experimental results, dechlorination of 2,4,6-trichlorophanol by the granules was conducted through 2,4-dichlorophenol, 4-chlorophenol or 2-chlorophenol to phenol at pH 7.0-7.2.

Alkanesulfonates↗

PubMed1995

Replacement of tryptophan residues in haloalkane dehalogenase reduces halide binding and catalytic activity.

Haloalkane dehalogenase catalyzes the hydrolytic cleavage of carbon-halogen bonds in short-chain haloalkanes. Two tryptophan residues of the enzyme (Trp125 and Trp175) form a halide-binding site in the active-site cavity, and were proposed to play a role in catalysis. The function of these residues was studied by replacing Trp125 with phenylalanine, glutamine or arginine and Trp175 by glutamine using site-directed mutagenesis. All mutants except Trp125-->Phe showed a more than 10-fold reduced kcat and much higher Km values with 1,2-dichloroethane and 1,2-dibromoethane than the wild-type enzyme. Fluorescence quenching experiments showed a decrease in the affinity of the mutant enzymes for halide ions. The 2H kinetic isotope effect observed with the wild-type enzyme in deuterium oxide was lost in the active mutants, except the Trp125-->Phe enzyme. The results indicate that both tryptophans are involved in stabilizing the transition state during the nucleophilic substitution reaction that causes carbon-halogen bond cleavage.

Amino Acid Sequence↗

PubMed1995

Palliative therapy of melanoma patients with fotemustine. Inverse relationship between tumour load and treatment effectiveness. A multicentre phase II trial of the EORTC-Melanoma Cooperative Group (MCG).

Fotemustine (FM) is a new chloronitrosurea (CNU), chemically characterized by the graft of an aminophosphonic acid on the CNU radical, which makes it highly lipophilic. Following single-institution phase I and II studies with remarkably high response rates of some 40%, including brain metastases of 25% and more, the EORTC-MCG started a multicentre phase II trial to confirm these results according to EORTC guidelines. Treatment consisted of an induction cycle of FM (100 mg/m2 on days 1 + 8 + 15), followed by maintenance courses (q3w). Fifty-four patients were entered by 11 institutions. General interest in this promising new agent, however, led clinicians of six additional institutions to join the EORTC trial and 90 more patients were included in only 4 months. This rapidly rising accrual rate became inversely related to the physicians' adherence to the eligibility criteria: palliation of symptoms rather than clinical research was the dominant reason to start treatment. Clinical characteristics and results in the eligible vs non-eligible patient group (in parentheses) were as follows: male/female 29/26 (68/65), mean age 54 years (53), ECOG-PS 0-1 (0-4), CR 2 (0), PR 10 (2), NC 17 (5) and for brain metastases: PR 4 (1), NC 2 (1), for an ORR of 12% (5%). Median duration of response was 6 months (range 4-16). The clinically relevant toxicity was limited to the haematopoiesis with delayed platelet nadirs (30% grade III+IV), granulocyte (25% grade III + IV) and the gastrointestinal tract: nausea and vomiting (26% grade II, 18% III, 1% IV). This study confirms that FM is active in melanoma including brain metastases.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗