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C Kruse

Publications and source records attributed to C Kruse.

39 records · Page 3Linked to original sources

Detection of hyperbilirubinemia by skin color measurements in icteric newborn infants at 5 to 14 days of age.

The relation between the yellow color of the skin, as read by the Minolta jaundice meter, and total bilirubin concentration, was compared in two groups of newborn infants suspected of having hyperbilirubinemia, but otherwise well. The study group comprised 73 neonates, icteric at the time of discharge from the nursery, and who were seen in the outpatient clinic at 5 to 14 days of age for checkup and blood sampling. The control group consisted of 52 neonates tested for hyperbilirubinemia at 1 to 4 days of age. Additionally, the relation between skin color and conjugated plasma bilirubin concentration was investigated in the study group. No significant differences in meter response to total bilirubin concentration was found between the two groups. The conjugated bilirubin concentration correlated significantly to the yellow skin color. The jaundice meter can be used as a test for hyperbilirubinemia in neonates at 5 to 14 days of age.

Bilirubin↗

Isolation of two immunosuppressive metabolites after in vitro metabolism of rapamycin.

Rapamycin was incubated with human liver microsomes and an NADPH regenerating system, the metabolites were purified by semipreparative HPLC, and their structures were elucidated by direct chemical ionization and FAB-MS. At least six fractions were isolated containing rapamycin metabolites, indicating that rapamycin is metabolized by the human liver cytochrome P-450 system. One of these metabolites was identified as 41-O-demethyl-rapamycin. A second metabolite was hydroxylated in a yet unknown position. These two metabolites retained immunosuppressive activity in a phytohemagglutinin-stimulated human lymphocyte assay with IC50S of 1 and 1.5 nmol/liter, respectively. Rapamycin was metabolized by rat small intestinal microsomes to at least two metabolites, indicating extra-hepatic metabolism of rapamycin.

Animals↗

Cochlear vascular changes in streptozotocin diabetes in chinchillas.

Chinchillas were made diabetic by streptozotocin intraperitoneal injection, and the cochlear blood vessels were studied using surface preparation techniques after 3, 6, and 12 months of established diabetes. The following findings were reported: (1) a decrease of alkaline phosphatase activity in the walls of cochlear capillaries after six months of established diabetes, (2) a generalized involvement of the cochlear microcirculation as evidenced by the decrease in alkaline phosphatase activity and by narrowing and irregularities of the capillary lumen, and (3) areas of complete capillary closure in the stria vascularis of the basal turn after 6 and 12 months of established diabetes.

Alkaline Phosphatase↗