Biomedical subjects

C Kuzmiak

Publications and source records attributed to C Kuzmiak.

3 recordsLinked to original sources

PubMed2001

Perspective on digital mammography.

Digital mammography, particularly through its advanced applications, holds great promise for improved diagnostic accuracy, but the display of the images is not ideal at present. Clinical softcopy workstations are somewhat unwieldy to use, and image processing has not yet been optimized for each machine or for each clinical task. In addition, the cost-effectiveness and accuracy of the technology warrant careful study before digital mammography becomes widely disseminated and potentially replaces screen-film mammography, a technology that has been well documented to reduce breast cancer mortality.

Clinical Trials as Topic↗

PubMed2000

Microtubule organization and the effects of GFP-tubulin expression in dictyostelium discoideum.

We have labeled microtubules in living Dictyostelium amoebae by incorporation of a GFP-alpha-tubulin fusion protein. The GFP-alpha-tubulin incorporates into microtubules and, as reported by others [Neujahr et al., 1998], the labeled microtubules are highly motile. Electron microscopy (EM) analysis of the distribution and organization of microtubules in the amoebae shows that some cytoplasmic microtubules form close associations. These associations could allow motor proteins attached to one microtubule to walk along an adjacent microtubule and thus generate some of the observed motility. Protein blot analysis indicates that the GFP-alpha-tubulin incorporates into microtubules at a lower efficiency than does the endogenous alpha-tubulin. EM and immunofluorescence (IF) analyses suggest that the GFP-alpha-tubulin interferes with microtubule nucleation. We have also observed an increased sensitivity of the GFP-alpha-tubulin expressing cells to blue light, as compared to wild-type cells. These results suggest that although GFP-alpha-tubulin can be used as a marker for microtubules in living cells, the use of this marker is not recommended for certain types of studies such as assembly dynamics.

Animals↗

PubMedDate not supplied

What every surgical oncologist should know about digital mammography.

This article reviews the available information on digital mammography for surgeons who care for patients with breast cancer. The limitations of the current film-based technology and why digital mammography promises to improve breast cancer detection and breast lesion diagnosis are described. The basics of digital imaging technology are reviewed, including a description of image contrast and spatial resolution and its variance from currently available clinical digital mammography systems. The results of clinical trials completed to date are reported. An upcoming large screening trial for digital mammography, sponsored by the National Cancer Institute, is described. Future technological developments, including improvements in softcopy display, image processing, computer-aided detection and diagnosis (CADD), tomosynthesis, and digital subtraction mammography (DSM), are briefly discussed.

Breast Neoplasms↗