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Biomedical subjects

C L Bailey

Publications and source records attributed to C L Bailey.

63 records · Page 4Linked to original sources

Winter survival of blood-fed and nonblood-fed Culex pipiens L.

Comparisons were made between groups of Culex pipiens L. with different physiologic histories to test their ability to sucessfully overwinter under field conditions. On 14 December 1978, each group of mosquitoes was marked with a distinctive fluorescent dust and released inside an abandoned ammunition bunker at Fort Washington, Maryland. To insure that dead mosquitoes could be dissected and information obtained on their ovarian development, a sample of females from each group was also released into a plexiglass cage that was attached to the inside wall of the room. The physiologic histories of each group of mosquitoes were as follows: (a) "wild caught", those which had entered the bunker prior to the release date, (b) "lab-reared diapausing nonblood-fed," (c) "lab-reared diapausing blood-fed nongravid, " (d)"lab-reared diapausing blood-fed gravid," (e) "lab-reared nondiapausing nonblood-fed," and (f) "lab-reared nondiapausing blood-fed." By 8 March 1979, all of the lab-reared nondiapausing groups, of mosquitoes released in the room had died, whereas 15.7, 22.4 and 24.7% were recovered from the "lab-reared diapausing nonblood-fed," "lab-reared diapausing blood-fed" (gravid and nongravid) and "wild caught" mosquitoes, respectively. For the mosquitoes in the cage, only 0, 2.1 and 7.0% of the "lab-reared nondiapausing blood-fed," "lab-reared nondiapausing nonblood-fed" and "lab-reared diapausing blood-fed gravid," respectively, survived. This compared to 45.4, 56.8 and 58.0%, respectively, for the "lab-reared diapausing nonblood-fed," "lab-reared diapausing blood-fed nongravid" and the "wild caught" groups. These data provide evidence to support the theory that a significant number of diapausing Cx. pipiens which have taken a prehibernation (possibly viremic) blood meal do not develop eggs and can survive the winter at rates comparable to diapausing nonblood-fed mosquitoes.

Animals↗

Serologic evidence of Jamestown Canyon and Keystone virus infection in vertebrates of the DelMarVa Peninsula.

Serological data accumulated during the past decade indicated that a variety of feral and domestic animals of the Delaware-Maryland-Virginia (DelMarVa) Peninsula were infected with Jamestown Canyon (JC) and/or Keystone (KEY) viruses (Bunyaviridae, California serogroup). Neutralizing (N) antibody to JC virus was most prevalent in white-tailed deer, sika deer, cottontail rabbits and horses. KEY virus N antibody was detected most frequently in gray squirrels and domestic goats. N antibody indicative of past infection by one or both viruses also was found in raccoons, horses and humans. JC and/or KEY virus N antibodies were not demonstrable in sera of several other species of small mammals and reptiles. Investigations were extended to evaluate the role of domestic goats as an amplifying host of JC and KEY viruses and to assess their potential as sentinels of virus transmission. Goats maintained in the Pocomoke Cypress Swamp during the summer season of 1978, acquired N antibodies to JC and KEY viruses. Following experimental inoculation with either JC or KEY virus, all goats developed N antibody despite the absence of a demonstrable viremia in most animals. Goats proved to be effective as sentinels for monitoring the transmission of JC and KEY viruses; however, the exceptionally low titers or absence of viremia following inoculation with these viruses would seem to preclude a potential virus-amplifying role for this species. Although findings implicated primarily gray squirrels and white-tailed deer as possible amplifying hosts of KEY and JC virus, respectively, further investigations will be required to clarify their role, particularly since both viruses may be maintained entirely by transovarial transmission.

Aedes↗

Spectral analysis of allophycocyanin I, II, III and B from Nostoc sp. phycobilisomes.

Low temperature (-196C) and room temperature (25C) absorption spectra of a family of allophycocyanin spectral forms isolated from Nostoc sp. phycobilisomes as well as of the phycobilisomes themselves have been analyzed by Gaussian curve-fitting. Allophycocyanin I and B share long wavelength components at 668 and 679 nm, bands that are absent from allophycocyanin II and III. These long wavelength absorption components are apparently responsible for the 20 nm difference between the 680 nm fluorescence emission maximum of allophycocyanin I and B and the 660 nm maximum of II and III. This indicates that allophycocyanin I and B are the final acceptors of excitation energy in the phycobilisome and the excitation energy transfer bridge linking the phycobilisome with the chlorophyll-containing thylakoid membranes. These Gaussian components are also found in resolved spectra of phycobilisomes, are arguing against this family of allophycocyanin molecules being artifactual products of protein purification procedures.

Cyanobacteria↗