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Biomedical subjects

C L Cole

Publications and source records attributed to C L Cole.

14 recordsLinked to original sources
PubMed1996

The lobster shaw gene: cloning, sequence analysis and comparison to fly shaw.

We cloned and sequenced the cDNA for the shaw gene, encoding a voltage-dependent potassium (K+) channel, from the spiny lobster, Panulirus interruptus. The deduced amino acid sequence has a high degree of homology to the Drosophila melanogaster Shaw protein. In addition, lobster Shaw has several putative sites for post-translational modifications.

Amino Acid Sequence↗
PubMed1996

Lobster shal: comparison with Drosophila shal and native potassium currents in identified neurons.

The transient potassium (K+) current, or A-current (IA), plays an essential role in shaping the firing properties of identified neurons in the 14-cell pyloric network in the stomatogastric ganglion of the spiny lobster, Panulirus interruptus. The different cells in the pyloric network have distinct IAs. To begin to understand the molecular basis for IA heterogeneity, we examined the relationship between the Panulirus shal current, the IAs in the lateral pyloric (LP) and pyloric dilator (PY) cells, and the Drosophila shal current. After isolating a complete open reading frame for lobster shal 1, which shows significant sequence homology to the fly, mouse, and rat shal homologs, we used a single-cell reverse transcription polymerase chain reaction method to demonstrate that the shal 1 gene was expressed in the LP and PY cells. Next, we compared the lobster shal 1 current generated in a Xenopus oocyte expression system to the IAs in the LP and PY neurons as well as to the Drosophila shal current in Xenopus oocytes. While the transient K+ lobster shal 1 current was similar to the IAs in pyloric neurons, a detailed comparison shows that they are not identical and differ in kinetic and voltage-dependent parameters. The highly homologous lobster and fly shal genes also produce currents with some significant similarities and differences in an oocyte expression system.

Amino Acid Sequence↗
PubMed1996

RT-PCR analysis of shaker, shab, shaw, and shal gene expression in single neurons and glial cells.

We have developed a reverse transcription-polymerase chain reaction (RT-PCR) method to examine single neurons and glial cells in the stomatogastric ganglion of the spiny lobster Panulirus interruptus for the expression of four members of the Shaker family of potassium channel genes. Using this technique we found that shaker, shab, shaw, and shal are expressed in 100%, 78%, 100%, and 94% of stomatogastric neurons. Furthermore, neuronal shab, shaw, and shal transcript levels vary among cells in a manner which is independent of cell size. We also detected Shaker family gene expression in glial cells. Shaker, shaw, and shal are detectably expressed in 100%, 63%, and 100% of the glial caps examined, respectively, while shab gene expression could not be detected in glial cells.

Animals↗
PubMed1994

Shab gene expression in identified neurons of the pyloric network in the lobster stomatogastric ganglion.

A single shab gene exists in the lobster, Panulirus interruptus, and undergoes alternate splicing to produce multiple transcripts. Using in situ hybridization we have determined the expression pattern of the shab gene in identified neurons of the pyloric network. The shab gene is consistently expressed at a low level in the Ventricular Dilator cell, a high level in the Pyloric Dilator cell, and is not detectably expressed in the Lateral Pyloric or Inferior Cardiac cells. Shab gene expression in the Anterior Burster cell varies from animal to animal. The electrophysiologically heterogeneous group of eight Pyloric Constrictor cells also shows differences in shab gene expression. These results support the idea that differences in shab gene expression contribute to the unique electrophysiological phenotypes displayed by each cell type.

Amino Acid Sequence↗
PubMed1985

Self-management training of mentally retarded adults presenting severe conduct difficulties.

Chronic and clinically significant conduct difficulties of mildly and moderately mentally retarded adults in a vocational setting were reduced with a multicomponent self-management package. Skills of self-monitoring, self-evaluation, self-consequation, and self-instruction were trained and practiced in vivo. Positive effects on behaviors not specifically treated also were noted for some subjects. A combined treatment withdrawal and multiple baseline design was used to assess changes. Nine-month follow-up under different work conditions revealed continued maintenance of treatment gains.

Adult↗
PubMed1983

Self-management of disruptive verbal ruminations by a mentally retarded adult.

Chronic and high-rate disruptive verbal ruminations of a mentally retarded adult in a vocational training setting were successfully reduced following introduction of a multi-component, self-management intervention program. Self-management skills of self-monitoring, self-evaluation, self-consequation, and self-instruction were trained and then practiced in vivo. In addition to influencing the specific dependent disruptive rumination behaviors targeted for intervention, three collateral behaviors not specifically treated showed positive effects. A combined treatment withdrawal and modified changing criterion design was used to assess changes throughout phases of the study. Follow-up data obtained 6 months and again 12 months after termination of the program revealed durability of intervention effects. The practical, conceptual, and philosophic significance of these findings are noted.

Adult↗
PubMed1975

Mate swapping: Perceptions, value orientations, and participation in a midwestern community.

This article attempts to illuminate the understanding of swinging, or mate swapping, an increasingly common form of extramarital sexual activity. A theoretical formulation argues that swinging is a form of extramarital sexual activity which serves to define as good and acceptable a behavior that in other forms and in the past has been considered deviant or immoral. A stratified area probability sample of 579 married adults was drawn from a Midwestern community of 40,000. Areas investigated included community knowledge and perception of swinging, values of respondents with regard to participation in and acceptance of swinging, and the incidence of swinging in the community. Most respondents dispproved of mate swapping as well as other forms of extramarital sex. Over half of the respondents knew about mate swapping, although less than 7% of the sample would consider participating. Swinging was found to exist in the community, but less than 2% of the respondents had ever participted. The data analysis is descriptive and exploratory, focusing on social correlates and characterisitics.

Adolescent↗