Safety aspects in preparing suspensions of field strains of Brucella abortus for serological identification.
Forty field strains of Brucella abortus, obtained from milk and vaginal samples of naturally infected cattle, were heated in a waterbath at 65 degrees C, 75 degrees C, 80 degrees C for 60 to 120 minutes. Ninety-five per cent survived 65 degrees C for 120 minutes, 55 per cent survived 75 degrees C for 120 minutes, 25 per cent survived 80 degrees C for 120 minutes and 12.5 per cent survived 85 degrees C for 60 minutes. No isolates survived 85 degrees C for 75 minutes.
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