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C L Garrett

Publications and source records attributed to C L Garrett.

5 recordsLinked to original sources

Heat distribution in the lower leg from pulsed short-wave diathermy and ultrasound treatments.

OBJECTIVE: To compare tissue temperature rise and decay after 20-minute diathermy and ultrasound treatments. DESIGN AND SETTING: We inserted 3 26-gauge thermistor microprobes into the medial aspect of the anesthetized triceps surae muscle at a depth of 3 cm and spaced 5 cm apart. Eight subjects received the diathermy treatment first, followed by the ultrasound treatment. This sequence was reversed for the remaining 8 subjects. The diathermy was applied at a frequency of 27.12 MHz at the following settings: 800 bursts per second, 400-microsecond burst duration, 850-microsecond interburst interval, peak root mean square amplitude of 150 W per burst, and an average root mean square output of 48 W per burst. The ultrasound was delivered at a frequency of 1 MHz and an intensity of 1.5 W/cm(2) in the continuous mode for 20 minutes over an area of 40 times the effective radiating area. The study was performed in a ventilated research laboratory. SUBJECTS: Sixteen (11 men, 5 women) healthy subjects (mean age = 23.56 +/- 4.73 years) volunteered to participate in this study. MEASUREMENTS: We recorded baseline, final, and decay temperatures for each of the 3 sites. RESULTS: The average temperature increases over baseline temperature after pulsed short-wave diathermy were 3.02 degrees C +/- 1.02 degrees C in site 1, 4.58 degrees C +/- 0.87 degrees C in site 2, and 3.28 degrees C +/- 1.64 degrees C in site 3. The average temperature increases over baseline temperature after ultrasound were only 0.17 degrees C +/- 0.40 degrees C, 0.09 degrees C +/- 0.56 degrees C, and -0.43 degrees C +/- 0.41 degrees C in sites 1, 2, and 3, respectively. The temperature dropped only 1 degrees C in 7.65 +/- 4.96 minutes after pulsed short-wave diathermy. CONCLUSIONS: We conclude that pulsed short-wave diathermy was more effective than 1-MHz ultrasound in heating a large muscle mass and resulted in the muscles' retaining heat longer.

Journal Article↗

A bacterial artificial chromosome-based framework contig map of human chromosome 22q.

We have constructed a physical map of human chromosome 22q using bacterial artificial chromosome (BAC) clones. The map consists of 613 chromosome 22-specific BAC clones that have been localized and assembled into contigs using 452 landmarks, 346 of which were previously ordered and mapped to specific regions of the q arm of the chromosome by means of chromosome 22-specific yeast artificial chromosome clones. The BAC-based map provides immediate access to clones that are stable and convenient for direct genome analysis. The approach to rapidly developing marker-specific BAC contigs is relatively straightforward and can be extended to generate scaffold BAC contig maps of the rest of the chromosomes. These contigs will provide substrates for sequencing the entire human genome. We discuss how to efficiently close contig gaps using the end sequences of BAC clone inserts.

Cell Line↗

A high-density YAC contig map of human chromosome 22.

We have constructed a high-resolution clone map of human chromosome 22 which integrates the available physical and genetic information, establishing a single consensus. The map consists of all classes of DNA landmarks ordered on 705 yeast artificial chromosomes (YACs) at an average landmark density of more than one per 70 kilobases. This map represents the practical limits of currently available YAC resources and provides the basis for determination of the entire gene content and genomic DNA sequence of human chromosome 22.

Chromosome Mapping↗