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Biomedical subjects

C L Moore

Publications and source records attributed to C L Moore.

At least 91 records · Page 5Linked to original sources
PubMed1977

The isolation of cerebral neurons with partial retention of processes.

A novel tissue disaggregation technique has been devised which permits the isolation of neurons with fairly extensive processes attached. Cortex is dissociated by aspiration through nozzles of decreasing size followed by agitation on a vortex mixer, rather than by the usual technique of forcing tissue through sieves. After each aspiration step, dissociated cells are separated from undisrupted tissue by coarse filtration and the latter is subjected to repeated treatment. This prevents unnecessary trauma to the free cells. After disruption is complete, small pieces of undisrupted tissue are removed from the cell suspension by floating on the foam created by degassing the suspension under vacuum. Cells are purified by conventional velocity-gradient centrifugation. This procedure has been applied successfully to fresh rat brain, with or without a preincubation with trypsin, frozen human brain and frozen bovine brain. The cell yields from rat brain were comparable to or better than, those obtained by other procedures (37 X 10(6) cells/g brain) while the purity was comparable. Cell yields from human brain were similar to those from rat brain but the purity was lower. The lowered particle purity of human and bovine cells can probably be attributed to the conditions of storage of the tissue and to trapping of free nuclei in the meshwork of dendritic processes. Values are given for the amount of protein, RNA and DNA per cell.

Animals↗
PubMed1977

Effects of triiodothyronine on oxidative phosphorylation in immature rat brain mitochondria.

Oxidative phosphorylation was measured polarographically in brain mitochondria isolated from 1 to 3-week-old normal and triiodothyronine-treated rat pups. Adenosine diphosphate (ADP)/oxygen ratios with nicotinamide-adenine dinucleotide (NAD)-linked substrates, but not with succinate, were increased in brain mitochondria from experimental animals at each age. Control ratios and respiratory rates were not affected. Thus, the normal maturational increase in ADP/oxygen ratios with NAD-linked substrates is accelerated in brain mitochondria from rats treated with triiodothyronnie from birth. This effect on efficiency of oxidative phosphorylation is similar to that of throid hormones on other properties of the maturing brain.

Age Factors↗
PubMed1977

Brief screening for developmentally delayed preschoolers.

23 preschool boys and girls with developmental delays were administered the Peabody-A, Riley Design, McCarthy Designs, and the Riley human figure. Correlations among scores ranged from .58 to .80 suggesting preliminary screening might be undertaken by a qualified teacher though this sample was very small and special.

Child↗
PubMed1976

Development of myelination in optic tract of the cat.

The postnatal development of myelin in the optic nerve and tract of normal and dark reared cats has been studied quantitatively with light and electron microscopy. In the newborn cat few myelinated fibers (3% of the population) are seen in the optic tract. Until the end of the second postnatal week, the total number of myelinated axons in the tract remains low (23%). At this time, however, there is an explosive increase in the rate of myelination and by the end of the fourth postnatal week 80% of the optic tract axons have acquired a myelin sheath. Thereafter, the number of myelinated axons increases gradually, reaching adult levels (100%) at 12 weeks. During the initial period of myelination, the average axon diameter is 0.6 mu for unmyelinated fibers and a.2 mu for myelinated fibers. Both of these means remain substantially unchanged until myelination is completed, suggesting that initial myelination of an axon is not a continuous process but rather proceeds in a step-wise manner. Dark rearing appears to have no effect on the initiation of myelination.

Aging↗
PubMed1976

The transition from sitting on eggs to sitting on young in ring doves, Streptopelia risoria: squab-egg preferences during the normal cycle.

Ring doves of both sexes sit on young squabs after hatching in much the same manner as they sit on eggs before hatching, but this study demonstrates that the preferred stimulus varies with the state of the animal. A simultaneous squab--egg choice test was given on days 1, 4, 10, and 13 of incubation and on the day following hatching in normal reproductive cycles of experienced and naïve male and female ring doves. Naïve doves were more likely than experienced doves to choose eggs throughout the cycle (P less than 0-005) and, overall, eggs were more likely to be chosen during early incubation and squabs posthatching (P less than 0-005).

Animals↗
PubMed1976

Experiential and hormonal conditions affect squab-egg choice in ring doves (Streptopelia risoria).

Noncycling, progesterone-injected, experienced male ring doves prefer eggs to squabs in a simultaneous squab-egg choice test, while experienced male doves injected with both progesterone and prolactin prefer squabs to eggs. When exogenous prolactin is administered to either naive or experienced male ring doves during the early phase of a normal incubation cycle, squab choice is significantly increased. Squab choice is also significantly increased in incubating doves by 24 hr of exposure to a squab in the home nest. This effect is greater in naive than in experienced doves. Squabs introduced during late incubation have more of a positive effect on squab choice than when introduced during early incubation. It is concluded that intracyclic experience organizes successive behavioral changes within a reproductive cycle and that naive doves are more affected by some changes in intracyclic events than are experienced doves.

Animals↗
PubMed1975

Ligandin reverses bilirubin inhibition of liver mitochondrial respiration in vitro.

Ligandin, an abundant cytoplasmic binding protein of bilirubin and other ligands in liver cells, completely prevented the inhibitory effect of bilirubin on respiration and oxidative phosphorylation by isolated rat liver mitochondria. At equimolar concentrations of bilirubin and ligandin or human serum albumin, mitochondrial respiration was fully restored to control values. At greater ratios of bilirubin and ligandin or human serum albumin, the latter had a stronger protective effect. These studies suggest that ligandin may have a physiologic role in protecting mitochondrial systems against bilirubin toxicity.

Animals↗
PubMed1973

Peroxisomal and mitochondrial defects in the cerebro-hepato-renal syndrome.

The cerebro-hepato-renal syndrome is a rare familial malady with cerebral, renal, and skeletal abnormalities, severe hypotonia, cirrhosis, iron and lipid storage, and death within 6 months. Correlated electron microscopic, histochemical, and biochemical studies demonstrate defects in two oxidative organelles. Peroxisomes cannot be found in hepatocytes and renal proximal tubules. In hepatocytes and cortical astrocytes, mitochondria are distorted in their appearance and glycogen stores are increased. Oxygen consumnption of brain and liver mitochondrial preparations with succinate and with substrates reducing nicotinamide adenine dinucleotide is markedly diminished, but the consumption is normal with ascorbate and tetramethylphenylenediamine, which suggests a defect in electron transport prior to the cytochromes. Histochemical studies of mitochondrial oxidation point to a defect between the succinate dehydrogenase flavoprotein and coenzyme Q, possibly in the region of nonheme iron protein.

Acidosis↗