Biomedical subjects
C Lawrence
Publications and source records attributed to C Lawrence.
A tale of two sciences: bedside and bench in twentieth-century Britain.
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System memory in violent incidents: evidence from patterns of reoccurrence.
For 20% of a sample of 1,078 violent incidents reported in British bars and pubs, another incident was reported at the same premises within 6 months. Log-survival analysis revealed nonrandom sequences that demonstrate a system memory effect separate from any biases involving particular venues. The rate of reoccurrence was not constant during the 6 months following incidents but was significantly higher for Weeks 1-4, approximated to the mean value for Weeks 5-12, and declined for Weeks 13-26. Reoccurrence was particularly likely in the first 3-4 days after an incident. Risk of reoccurrence was further increased for incidents that involved either threats or the exit and return of the assailants within that original incident. Results support the view that "violence breeds violence" and demonstrate the need for increased staff vigilance for up to 12 weeks following a violent incident, and particularly during the first few days and weeks.
The effects of income inequality on health.
Much of the discussion about individual and group differences in illness and life expectancy has focused on the effects of individual characteristics, both status and behavioural. This is also characteristic of much of the literature, which attempts to explain why men have higher rates of disease and lower life expectancy than women. After a period in which 'social policy was no longer such an important part of preventive health policy', there is now renewed interest in the influence of the socioeconomic environment on health. Indeed, recently compiled evidence indicates that increasing income inequality is likely to have adverse effects on the community's health. These findings highlight the potential dangers of policy changes which accelerate social and economic divisions.
Diffuse axonal injury in a rugby player.
Sections of the cerebrum, cerebellum, and brain stem of a rugby player who died 15 hours after being tackled were stained using an immunoperoxidase technique to detect beta-amyloid protein. The sections of the pons showed axonal spheroids in the base, and those of the cerebellum showed axonal spheroids in deep white matter. The findings demonstrated axonal injury following a fall from the victim's height in a team sporting event.
The valve of Vieussens: an important cause of difficulty in advancing catheters into the cardiac veins.
INTRODUCTION: The coronary sinus and cardiac veins are useful conduits for the passage of electrode catheters for mapping the origin of cardiac arrhythmias. However, sometimes it is difficult to advance catheters an adequate distance into the cardiac veins. The aim of this study was to determine the reasons for this. METHODS AND RESULTS: In 50 cadaveric hearts, a deflectable 7-French electrode catheter was passed from the right atrium into the coronary sinus and advanced to the anterior interventricular portion of the great cardiac vein (GCV). Causes of obstruction were determined. The catheter was obstructed by the valve of Vieussens in 23 of 50 hearts (46%). Once the valve was negotiated, obstruction was caused by an acute bend in the GCV in 28 of 50 hearts (56%). Clinical studies were undertaken in 10 patients in whom electrode catheters could not be advanced as far as required. Using contrast venography, the most frequent cause of obstruction was determined to be the valve of Vieussens in 8 of 10 cases (80%). An acute bend in the GCV caused obstruction in 2 cases (20%). CONCLUSIONS: The valve of Vieussens is a frequent cause of obstruction to passage of a catheter in postmortem and in vivo studies. An acute bend in the vein, with or without lodgment in a tributary, is the other common cause. In adults, venous luminal diameter is not a cause of obstruction to the passage of a 7-French catheter in the coronary sinus or proximal GCV.
Single clonal origin of a high proportion of Legionella pneumophila serogroup 1 isolates from patients and the environment in the area of Paris, France, over a 10-year period.
Arbitrarily primed PCR with three primers and pulsed-field gel electrophoresis were used to characterize a set of 75 clinical Legionella pneumophila serogroup 1 isolates, with no apparent epidemiological link, obtained from 24 hospitals in Paris, France, from 1987 to 1997. Unexpectedly, 25 clinical isolates from 15 hospitals had an identical profile (termed type A) by both methods. The same profile was subsequently found in 16 of 64 randomly selected environmental L. pneumophila serogroup 1 isolates from 15 different sites in the Paris area. There was no evidence of geographic clustering or a peak incidence of type A isolation. Type A has not been found in France outside the Paris area, suggesting that a particular type of L. pneumophila serogroup 1 is specifically present in the Paris water distribution network.
Group A streptococcal multifocal septic arthritis: a case report.
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Pitfalls of the golden rule in caregiving.
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Personal choices on private health insurance.
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Expression of feline recombinant interferon-gamma in baculovirus and demonstration of biological activity.
We have previously reported the cloning of the coding sequence for feline-specific interferon-gamma. Here, we describe the expression of this sequence in a baculovirus system and demonstrate the biological activity of the recombinant protein. The coding sequence for feline interferon was directionally cloned into the baculovirus transfer vector pAcCL29-1. Transfer vector and linearized wild-type AcMNPV (BacPAK6) were used to co-transfect Sf9 cells by calcium phosphate coprecipitation. Subsequently, wild-type and recombinant viruses were separated by plaque assay. Recombinant plaques were expanded and a master stock of virus is produced. Production of biologically active interferon-gamma from infected Sf9 cells was demonstrated using a standard cytopathic effect reduction assay, utilising vesicular stomatitis virus (VSV), and an MHC class II induction assay.
Temporal architecture of violent incidents.
Five hundred five reports of violent incidents in British pubs and bars were studied by using logical pathway modeling to provide information on the processes underlying work-related violence. Logical pathway modeling is innovative in examining and mapping sequences in real incidents at a population level. The data reveal the most common pathway to be misbehavior by customers, intervention by staff (before any physically violent act), physical attack on staff, and injury to staff. The data also highlight the likelihood of further action after assailants have exited and identify stages in incidents at which most staff and customer injuries and damage to property occur. Results assist in the design of strategies to reduce the risk from future violence, particularly by training staff to recognize and to handle potentially violent situations and to maintain vigilance and security following problem incidents.
Production of interleukin-12 by murine macrophages in response to bacterial peptidoglycan.
Peptidoglycan (PG), a component of the bacterial cell wall, has various immunomodulating activities, including the capacity to induce delayed-type hypersensitivity reactions to antigens administered in Freund's adjuvant. We report that PG induces interleukin-12 (IL-12) mRNA production and IL-12 secretion by mouse macrophages. The capacity of PG to induce IL-12 production, like its previously reported immunomodulating activities, was dependent on the structure of its peptide subunit. PG from Bacillus megaterium and Staphylococcus aureus induced IL-12 production, whereas PG from Micrococcus luteus and Corynebacterium poinsettiae did not. The ability of most bacterial PGs to induce IL-12 production suggests that they play an important role in triggering host defense mechanisms against bacterial infections.
Essential skills for paediatric nurses.
Concerns about the varied nature of practical learning, experiences available to students prompted this study of the skills required to nurse children. Experienced paediatric nurses were surveyed to identify which skills they considered to be essential and which desirable in child branch diplomates. Out of 74 practical skills. 47 were believed to be essential on qualification. This list of skills could provide an expected baseline for students to achieve during their educational programme.
[Comparative activity of cefepime, ceftazidime and imipenem in a mouse infection caused by Klebsiella pneumoniae producing a broad spectrum beta-lactamase].
Mice experimentally infected with extended-spectrum beta-lactamase-producing Klebsiella pneumoniae strains were injected twice daily for three days with ceftazidime, cefepime, or imipenem (25, 50, or 100 mg/kg/injection). Treatment efficacy was based on five-day survival and on the spleen viable bacteria count 16 hours after the last treatment dose. Under these experimental conditions, ceftazidime showed some activity on strains with low levels of resistance to ceftazidime. Cefepime used in a dose of 50 or 100 mg per injection demonstrated good activity but was slightly less effective than imipenem.
Molecular interactions between Hb alpha-G Philadelphia, HbC, and HbS: phenotypic implications for SC alpha-G Philadelphia disease.
We show here that alpha2(G-Phila.) beta2(C) has an increased rate of crystal nucleation compared to alpha2 beta2(C) (HbC). We conclude from this finding that position alpha68, the mutation site of alpha2(G-Phila.) beta2 (HbG(Philadelphia)), is a contact site in the crystal of HbC. In addition, that HbS enhances HbC crystallization (additive to the effect of alpha(G-Phila.) as shown here) and that alpha(G-Phila.) inhibits polymerization of HbS are pathogenically relevant previously known facts. All of these findings help explain the phenotype of an individual simultaneously heterozygous for the betaS, betaC, and the alpha(G-Phila.) genes (SC alpha-G Philadelphia disease). This disease is characterized by a mild clinical course, abundant circulating intraerythrocytic crystals, and increased folded red cells. This phenotype seems to be the result of increased crystallization and decreased polymerization brought about by the opposite effects of the gene product of the alpha(G-Phila.) gene on the betaC and betaS gene products. Some of the intraerythrocytic crystals in this syndrome are unusually long and thin, resembling sugar canes, unlike those seen in SC disease. The mild clinical course associated with increased crystallization implies that, in SC disease, polymerization of HbS is pathogenically more important than the crystallization induced by betaC chains. The SC alpha-G Philadelphia disease is an example of multiple hemoglobin chain interactions (epistatic effect among globin genes) creating a unique phenotype.
Non-mutagenic repair of (6-4)photoproducts by (6-4)photolyase purified from Drosophila melanogaster.
The (6-4)photoproduct DNA photolyase ((6-4)photolyase) repairs UV-induced pyrimidine (6-4) pyrimidone photoproduct ((6-4)photoproduct, pyr[6,4]pyr) in a light dependent manner. Drosophila (6-4)photolyase was purified to near homogeneity from Drosophila embryonic cells and is shown to be a 62 kDa protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified (6-4)photolyase repairs (6-4)photoproducts induced at 5'-CC-3' site (C[6,4]C) as well as T[6,4]T and T[6,4]C. Photoreactivation of (6-4)photoproduct constructed in M13 phage eliminates the replication block and abolishes induced mutagenesis in E. coli cells, suggesting that the (6-4)photolyase repairs the photoproduct to the unmodified form.