Intracranial granuloma caused by asporogenic Aspergillus fumigatus in a harbour porpoise (Phocoena phocoena).
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Animals↗
Biomedical subjects
C Linton
Publications and source records attributed to C Linton.
Isotope effects and Born-Oppenheimer breakdown in excited singlet states of the lithium dimer.
Observation of infrared electronic transitions involving the 1 (1)Deltag state of 7Li2 has instigated an investigation of Born-Oppenheimer breakdown in four singlet electronic states correlating with (2s+2s), (2s+2p), and (2p+2p) lithium atoms. The 1 (1)Deltag state, which correlates at long range with (2p+2p) atoms, has been observed in emission from the (5p) (1)Piu Rydberg state and in 1 (1)Deltag-B (1)Piu bands, in both instances following optical-optical double-resonance excitation. The latter transition was observed previously for the lighter isotopomer, 6Li2 [C. Linton, F. Martin, P. Crozet, A. J. Ross, and R. Bacis, J. Mol. Spectrosc. 158, 445 (1993)]. By analyzing multiple-isotopomer data for several electronic systems simultaneously, we have determined the electronic isotope shifts and the leading vibrational and/or rotational Born-Oppenheimer breakdown terms for the X (1)Sigmag+, A (1)Sigmau+, B (1)Piu, and 1 (1)Deltag states of the lithium dimer. This paper also reports Fourier transform measurements of the B-X absorption spectra of 6Li2 and 7Li2, which were required to better define the bottom portion of the B (1)Piu state potential.
Journal Article↗
Travel-induced psychosis in the elderly.
We present three cases of travel-induced psychosis in an elderly population. The clinical presentations are described with a review of the literature.
Affective Disorders, Psychotic↗
High-Resolution Laser Spectroscopy of YbCl: The A(2)Pi-X(2)Sigma(+) Transition.
The A(2)Pi-X(2)Sigma(+) transition of (174)Yb(35)Cl and (172)Yb(35)Cl has been rotationally analyzed for the first time. Doppler-limited laser excitation spectroscopy with selective detection of fluorescence was used to obtain spectra of the 0-0 and 1-0 bands with a measurement accuracy of approximately 0.0035 cm(-1). Resolved fluorescence was used to record the 0-1, 0-2, and 0-3 bands and to unequivocally assign the rotational numbering, N, to the laser excitation spectra. In total, over 1300 line positions have been measured and assigned for each of the two isotopomers and employed in least-squares fits of molecular parameters. The principal results for the A(2)Pi state are A(e) = 1491.494(2) cm(-1) and R(e) = 2.4433(1) Å, and for the X(2)Sigma(+) state, R(e) = 2.4883(2) Å and gamma(e) = 4.59(2) x 10(-3) cm(-1). The interaction between the X(2)Sigma(+) and A(2)Pi states has been investigated and is shown to be the main contributor to the spin-rotation splitting in the ground state. Copyright 2000 Academic Press.
Journal Article↗
Predissociation in the B0(+) State of YbS and a Combined Isotopomer Fit to the X0(+) State Potential Energy Curve.
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Journal Article↗
The High-Lying Vibrational Levels and Dissociation Energy of the a3Sigma+u State of 7Li2.
Perturbation-facilitated optical-optical double resonance (PFOODR) has been used to access the 2(3)Pig state of 7Li2 via the excitation scheme using two single-mode tunable lasers. The selected () mixed level provides a gateway through which the triplet manifold can be accessed. Fluorescence from single rovibrational levels of 2(3)Pig to the state was detected at high resolution using a Fourier transform spectrometer. Transitions to v = 0-9 in the state were observed, covering the potential well almost to the dissociation limit. The data were analyzed using a near dissociation expansion (NDE) technique and the resulting vibrational and rotational parameters were used to calculate a new RKR potential curve which reproduced the observed energy levels to within a rms error of 0.02 cm-1. The following parameters were obtained for the state: D0 = 301.829 +/- 0.015 cm-1, De = 333.69 +/- 0.10 cm-1, Te = 8183.12 +/- 0.12 cm-1. Copyright 1999 Academic Press.
Journal Article↗
On the 5d 1Pig --> 2 (1)Sigma+ u and 5d 1Pig --> C 1Piu Fluorescence in 7Li2.
Following excitation of the 5d 1Pig Rydberg state of 7Li2 by optical-optical double resonance, fluorescence has been observed in the infrared region to the 2 (1)Sigma+ u and C 1Piu states. Analysis of high-resolution Fourier transform spectra yields term energies and rotational constants of the lowest seven vibrational levels of the inner well of the 2 (1)Sigma+ u "double minimum" state. The equilibrium term value and dissociation energy have been determined to be Te = 30101.45 +/- 0.12 cm-1 and De = 5621.3 +/- 0.2 cm-1. The v = 0 and 1 levels of the C 1Piu state have been analyzed, resulting in new values of Te = 30551.0 +/- 0.1 cm-1 and De = 7773.3 +/- 0.2 cm-1. Copyright 1998 Academic Press.
Journal Article↗
Changes in Bcl-2 and p53 expression in recurrent B-cell lymphomas.
The aim of this study was to investigate the changes involved in the evolution of nine cases of recurrent B-cell lymphomas. Using the polymerase chain reaction (PCR) on formalin-fixed, paraffin-embedded tissue from both the primary and the recurrent lymphoma of each case, monoclonality was demonstrated in every tumour. In all nine cases, the recurrent lymphoma was shown to belong to the same clone as the primary lymphoma. Eight of these cases were then investigated by immunohistochemistry for changes in Bcl-2 and p53 expression. Five out of eight of the primary lymphomas showed Bcl-2 overexpression. Two of the three cases initially negative for Bcl-2 expression became positive in the recurrence. One out of eight of the primary lymphomas was positive for p53 expression. Of the seven negative cases, one became positive for p53 expression in the recurrence. Both of the p53-positive cases showed high-grade histology. This study shows that Bcl-2 overexpression is probably an important early event in the development of B-cell lymphomas, although it may occur as a post-neoplastic event. p53 mutation is probably more important as a late event and may be associated with high-grade transformation.
Gene Expression Regulation, Neoplastic↗
The use of the polymerase chain reaction in the diagnosis of B-cell lymphomas from formalin-fixed paraffin-embedded tissue.
In this study the use of the polymerase chain reaction (PCR) to detect monoclonality in B-cell lymphoid proliferations in archival formalin-fixed paraffin-embedded tissue was assessed. Using consensus primers against the framework 3 (FR 3) region of the immunoglobulin heavy chain gene (IgH), PCR analysis was performed on 29 low grade B-cell non-Hodgkin's lymphomas. Cases of benign lymphoid hyperplasia served as polyclonal controls. Sequenced cases of acute lymphoblastic leukaemia served as positive controls. In the lymphomas, monoclonality could be demonstrated in 18 of 29 (62%) cases. Only five of 11 (45%) follicle centre cell lymphomas were positive by this method whilst the success rate for the remainder was 13 of 18 (72%). None of the polyclonal controls gave false positive results although occasional non-specific dominant bands were present which disappeared on repeating the experiments. These results show that this method will identify monoclonality in 62% of low grade B-cell non-Hodgkin's lymphomas in archival material. The success rate is increased to 72% if follicle centre cell lymphomas are excluded. Thus, this method is a useful adjunctive test to aid diagnosis in lymphoid infiltrates when standard morphology and immunohistochemistry are equivocal.
Base Sequence↗
Neuroprotective properties of lifarizine compared with those of other agents in a mouse model of focal cerebral ischaemia.
1. Changes in the peripheral type benzodiazepine binding site density following middle cerebral artery occlusion in the mouse, have been used as a marker of neuronal damage. These sites can be identified using the selective ligand [3H]-PK 11195 located on non neuronal cells, macrophages and astroglia, within the CNS. Glial cell proliferation and macrophage invasion is an unvoidable sequelae to cerebral ischaemic injury, secondary to neuronal loss. Following occlusion of the left middle cerebral artery (left MCA) a reproducible lesion was found in the parietal cortex within 7 days which gave rise to a significant increase in [3H]-PK 11195 binding. 2. Treatment of animals with the sodium channel blocker, lifarizine, significantly reduced the ischaemia-induced increase in [3H]-PK 11195 binding when given either 30 min pre-ischaemia and three times daily for 7 days at 0.5 mg kg-1, i.p. (P < 0.01) or delayed until 15 min post-ischaemia and three times daily for 7 days at 0.5 mg kg-1, i.p. (P < 0.001). Lifarizine was an effective neuroprotective agent in this model of focal ischaemia in the mouse. 3. Lifarizine also showed a dose-related protection against the ischaemia-induced increase in [3H]-PK 11195 binding with significant protection at doses of 0.1 mg kg-1, i.p. (P < 0.05), 0.25 mg kg-1, i.p. (P < 0.01) or 0.5 mg kg-1, i.p. (P < 0.01) 15 min post-ischaemia and b.i.d. for 7 days. No significant change is seen in the Kd for [3H]-PK 11195. The first dose could be delayed for up to 4 h after cerebralartery cauterization and protection was maintained.4. Phenytoin (28 mg kg-1, i.v. 15 min and 24 h post-ischaemia) was also neuroprotective in this model(P<0.01). This agent is thought to interact with voltage-dependent sodium channels to effect its anticonvulsantactions and this mechanism may also underlie its neuroprotective actions in focal cerebralischaemia.5. Agents with other mechanisms of action were also shown to have significant neuroprotection in this model. The non-competitive NMDA antagonist, MK 801, showed significant neuroprotection in the model when given at 0.5 mg kg-1, i.p. 30 min pre-ischaemia with t.i.d. dosing for 7 days (P< 0.001). The dihydropyridine calcium antagonist, nimodipine was not protective when given using the same dosing protocol as MK 801, 0.5 mg kg-1 30 min pre-occlusion and three times daily for 7 days but showed significant protection when given at 0.05 mg kg-1 15 min post-ischaemia and three times daily for 7days. The lipid peroxidation inhibitor, tirilazad (single dose 1 mg kg-1, i.v.) showed significant neuroprotection when given 5 min post-ischaemia but not when the first dose was delayed for 4 h.
Animals↗
RS-45041-190: a selective, high-affinity ligand for I2 imidazoline receptors.
1. RS-45041-190 (4-chloro-2-(imidazolin-2-yl)isoindoline) showed high affinity for I2 imidazoline receptors labelled by [3H]-idazoxan in rat (pKi = 8.66 +/- 0.09), rabbit (pKi = 9.37 +/- 0.07), dog (pKi = 9.32 +/- 0.18) and baboon kidney (pKi = 8.85 +/- 0.12), but had very low affinity for alpha 2-adrenoceptors in rat cerebral cortex (pKi = 5.7 +/- 0.09). 2. RS-45041-190 showed low affinity for other adrenoceptors, dopamine, 5-hydroxytryptamine, and muscarinic receptors and dihydropyridine binding sites (selectivity ratio > 1000). 3. RS-45041-190 showed moderate potency for the inhibition of monoamine oxidase A in vitro (pIC50 = 6.12), but had much lower potency for monoamine oxidase B (pIC50 = 4.47), neither of which equated with its affinity for I2 receptors. 4. RS-45041-190 (0.001 to 3 mg kg-1, i.v. and 1 ng-50 micrograms i.c.v.) had only small, transient effects on blood pressure and heart rate in anaesthetized rats. In conscious rats, RS-45041-190 had no effect on body core temperature or tail skin temperature (1 mg kg-1, s.c.) or on activity or rotarod performance (10 mg kg-1, i.p.). There were also no effects on barbiturate sleeping time in mice after doses of 1-10 mg kg-1, i.p. 5. RS-45041-190 (10 and 25 mg kg-1, i.p.) significantly increased food consumption in rats for up to 4 h after dosing, but unlike idazoxan (10 mg kg-1, i.p.) did not increase water consumption. RS-45041-190 is therefore a selective, high-affinity ligand at I2 imidazoline receptors and its hyperphagic effect may suggest a role for I2 imidazoline receptors in the modulation of appetite.However, in the absence of a selective agonist it is unclear whether this ligand is an agonist or an antagonist at I2 receptors.
Adrenergic alpha-Antagonists↗
Absence of Mycobacterium tuberculosis DNA in synovial fluid from patients with rheumatoid arthritis.
OBJECTIVES: To determine whether Mycobacterium tuberculosis DNA can be detected in synovial fluid of patients with rheumatoid arthritis (RA). METHODS: The polymerase chain reaction was applied to cellular components of synovial fluid. RESULTS: No evidence of M tuberculosis DNA was found in synovial fluid from 31 patients with RA and 13 control patients. CONCLUSION: The findings do not support a role for persistent M tuberculosis infection in the pathogenesis of RA.
Adolescent↗