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C Lobo

Publications and source records attributed to C Lobo.

26 records · Page 2Linked to original sources
PubMed1995

Glutamine-glutamate exchange between placenta and fetal liver.

The hypothesis that glutamine shuttles nitrogen between placenta and fetal liver via interconversion with glutamate was explored by infusing L-[1,2-13C2]glutamine in six fetal sheep chronically catheterized for sampling of the umbilical and hepatic circulations. Fetal plasma glutamine disposal rate was 19.9 +/- 1.3 mumol.min-1.kg fetus-1. Entry of glutamine from the placenta accounted for approximately 60% of the total glutamine entry rate in fetal plasma. Glutamine was taken up by fetal liver, and 45.3 +/- 7.9% of the glutamine taken up was released as glutamate. The fetal liver released large quantities of glutamate, as evidenced by a sixfold increase in plasma glutamate concentration in the blood flowing through the left hepatic lobe and a hepatic glutamate output-to-O2 uptake molar ratio of 0.149 +/- 0.013. In conjunction with a previous study of fetal glutamate metabolism, these data demonstrate that glutamine entering the fetal circulation is converted to glutamate by the fetal liver at a rate of approximately 3-4 mumol.min-1.kg fetus-1.

Animals↗
PubMed1991

[Pulmonary Kaposi's sarcoma in a heterosexual parenteral drug addict patient with the acquired immunodeficiency syndrome].

A case of Kaposi syndrome is described in a 28-year-old heterosexual male with acquired immunodeficiency syndrome. The disease began clinically with pulmonary disease, without mucocutaneous lesions. This form of presentation is extremely infrequent and has not been described in non-homosexual subjects. Clinical manifestations were fever, cough and dyspnea. Thoracic radiography observed a perihilar interstitial pattern which evolved to a bilateral nodular pattern with perihilar adenopathy. There was endobronchial disease, however pulmonary biopsy was required for diagnosis. Complete tumoral remission was achieved with adriamycin, bleomycin and vincristine.

Acquired Immunodeficiency Syndrome↗
PubMed1981

[Sycosis of the beard (tinea barbae). Analysis of 42 cases].

42 patients suffering from dermatophytic fungus infection involving the bearded area, face and neck (tinea barbae) were seen in the Clinic between 1970 and 1977. The clinical diagnosis was confirmed by microscopic examination (KOH) of skin and hair scrapings in all the patients. The material obtained from 39 patients and inoculated gave a positive culture; in were isolated and following fungi: T rubrum-18, T. mentagrophytes-16, T. megninii-1, T. violaceum-2, M. canis-2. From the clinical point of veiw it was possible to classify the patients in 3 main types: --Superficial type-11 patients. --Kerion-10 patients (T. mentagrophytes-9, M. canis-1). --Nodular type-deep seated nodules, without discharging, very slow evolution - 21 patients (T. mentagrophytes-4, T. rubrum-13, T. megninii-1, T. violaceum-1). It was obtained material for histology in 13 patients: 3 suffering from kerion and 10 with the nodular clinical type. The histology of the Kerion type showed a marked inflammatory reaction consisting largely of neutrophils surrounding the hair follicles. Only in one examination, out of the 3 patients studied with Kerion, showed fungi, on PAS. The 10 patients suffering from the nodular type of infection who were studied histologically showed a more or less uniform picture, which consisted in a more deep seated infiltration specially consisting of lymphocytes and plasmocytes, on the derme but not so perifollicular as in Kerion and, in 2 cases, there was a tendency to form a tuberculoid structure. The PAS was positive in 6 patients. The fungi were always intrafollicular. All the patients were treated with griseofulvin 1 gr. per day. All of them healed after a period of 4-8 weeks of treatment.

Adolescent↗
PubMedDate not supplied

Effect of dehydrodidemnin B on human colon carcinoma cell lines.

Didemnins are cytotoxic agents belonging to a depsipeptide family isolated from marine tunicates. In the present study, a new member, dehydrodidemnin B (DDB), isolated from the mediterranean tunicate Aplidium albicans, was used. The effect of the drug on human colon cultured cell lines was tested using multiple approaches: proliferation studies, long term survival after three hours of exposure to DDB by means of a clonogenic assay and the decrease of the protooncogen, ornithine decarboxylase, activity. A dehydrodidemnin B concentration of 10(-8) M completely inhibited cell growth. The IC50 obtained using the MTT proliferation test, indicated that the most proliferative cell line (CT-2) was the most sensitive to the drug. Using a clonogenic assay a clear dose-response was obtained for the three cell lines used; HT-29 cell line showed the minimum survival after 3 hours of dehydrodidemnin B treatment. A dose-dependent decrease in ornithine decarboxylase activity was also observed in three cell lines assayed. The data presented indicate that the dehydrodidemnin B is a potent cytotoxic agent on rapidly dividing human colon cancer cells.

Antineoplastic Agents↗