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C Lugowski

Publications and source records attributed to C Lugowski.

51 records · Page 3Linked to original sources
PubMed1981

Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates.

The successful coupling of the meningococcal groups A, B, and C polysaccharides to tetanus toxoid to yield water soluble conjugates is described. Reactive aldehyde groups were strategically introduced into the terminal residues of the polysaccharides by the controlled periodate oxidation of the native groups B and C polysaccharides and of the group A polysaccharide previously modified by the reduction of its terminal reducing N-acetyl-mannosamine residue. This produced essentially monovalent polysaccharide molecules, which were subsequently covalently linked to tetanus toxoid by means of reductive amination. Although the groups A and C polysaccharides proved to be poor immunogens in rabbits and mice, their tetanus toxoid conjugates produced high levels of polysaccharide-specific antibodies in both animals. By contrast, even in the form of its tetanus toxoid conjugate, the group B polysaccharide failed to elicit homologous polysaccharide-specific antibodies in either animal; a major proportion of the antibodies actually produced had a specificity for the linkage area of the conjugate. This evidence is compatible with the hypothesis of the poor immunogenicity of the group B polysaccharide being structure related. Hyperimmunization of mice with the groups A and C polysaccharide-tetanus toxoid conjugates produced antisera with good bactericidal activity against their respective homologous organisms, and indicated the potential of these conjugates as potential human vaccines.

Aldehydes↗
PubMed1980

Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1.

The complex cell-wall polysaccharide, C-substance, was isolated from Streptococcus pneumoniae type 1 and purified by DEAE-cellulose (HCO3(-) form) and Sephadex column chromatography. The complete structure of this antigen was obtained by the application of methylation and 1H NMR and 13C NMR spectroscopic techniques to a series of oligosaccharide fragments obtained by the selective degradation of the N-acetylated antigen. Native C-substance is composed of the following repeating unit: beta-D-Glup-1 leads to 3-alpha-AAT-Galp-1 leads to 4-alpha-D-GalNAcp-1 leads to 3-beta-D-GalNH2p-1 leads to 1'-ribitol-5-phosphate where AATGal is 2-acetamido-4-amino-2,4,6-trideoxygalactose. Phosphocholine substituents are situated at O(6) of the unacetylated galactosamine residues, and the repeating units are linked through a diphosphate ester from ribitol to O(6) of the beta-D-glucopyranose residue. This structure has also been shown to be common to C-substances prepared from a number of other pneumococcal types based on the criterion of their identical 13C NMR spectra.

Carbohydrate Conformation↗
PubMed1978

Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization.

In the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from Shigella sonnei has been elaborated. The method is based on sonification of bacterial suspension in the presence of lysozyme and EDTA and subsequent extraction of the pellet with boiling water. The crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and Sephadex LH-20. The comparison of several extraction procedures of enterobacterial common antigen from Shigella sonnei proved that the method described above is most effective. The purified enterobacterial common antigen preparation obtained preserved full biological activity: antigenicity (precipitation and activity in enzyme-linked immunosorbent assay), immunogenicity in rabbits, ability to coat erythrocytes (passive hemagglutination) and inhibitory activity in passive hemagglutination. The pure enterobacterial common antigen was identified to 90% as a polymer of N-acetyl-D-mannosaminuronic acid and N-acetyl-D-glucosamine (2:1, molar ratio), O-acetylated and containing 3.2% fatty acids (C16:0 and C18:1, not oleic). It contains 5.3% nitrogen, less than 4% protein, less than 0.5% phosphorus and less than 1.6% neutral sugar; glycerol and RNA were not found in the preparation.

Antigen-Antibody Reactions↗
PubMed1978

Immunochemical characteristics of Shigella sonnei and serotype 6 Shigella flexneri lipopolysaccharides and enterobacterial common antigen.

Immunochemical studies on Shigella sonnei and serotype 6 Shigella flexneri 0 antigens (lipopolysaccharides) and enterobacterial common antigen (ECA) isolated from Shigella sonnei were carried out. Oligosaccharide structure of 0-specific chain of serotype 6 Shigella fiexneri lipopolysaccharide was defined and beta-L-rhamnosyl-1,3-N-acetyl-D-galactosamin as immunodeterminant of type VI specificity was recognized. The structures of core regions of lipopolysaccharides isolated from R mutants of both Shigella subgroups were established. On the base of the serological and structural results it has been suggested that these core regions are identical and very close to RI core structure of E. coli C. The effective method of isolation and purification of enterobacterial common antigen from Shigella sonnei was elaborated and its immunological properties as well as chemical character defined.

Antigens, Bacterial↗
PubMed1975

Immunochemical studies on the serotype of Shigella flexneri and its recombinant.

Chemical and serologic analysis of polysaccharides of Shigella flexneri 2b and its recombinant revealed complete defect of the gene responsible for type II specificity as a result of recombination. At the same time, function of the gene in the same locus responsible for 7,8 group specificity was partially inhibited. Attenuation of group antigen 7,8, associated with partial uncovering of the structure of the basic chain, causes appearance of a distinct 3,4 group antigen. Changes of this type in Shigella flexneri have not been described hitherto.

Conjugation, Genetic↗
PubMedDate not supplied

[An antigenic analysis and the protective properties of the R-form lipopolysaccharides of gram-negative bacteria].

The antigenic and immunogenic properties of the R-form lipopolysaccharides (R-LPS) of Pseudomonas aeruginosa, Salmonella minnesota, Escherichia coli and Shigella were studied. The results of the study revealed the existence of antigenic relationship between P.aeruginosa R-LPS and R-LPS Escherichia and Shigella. In serological tests no antigenic relationship between P. aeruginosa R-LPS and Salmonella R-LPS was revealed, but as shown in earlier experiments of the protection of mice, Re-glycolipid stimulated protective immunity against Pseudomonas infection in the animals. On the basis of R-LPS obtained from selected P. aeruginosa and Salmonella strains a vaccine was prepared which proved to be effective against infection caused by P. aeruginosa S-strain in experiments on mice. The vaccine induced protection in 40-100% of immunized mice, depending on the scheme of immunization. The vaccine may probably be effective against infections caused by other gram-negative bacteria.

Animals↗