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Biomedical subjects

C M Caldarera

Publications and source records attributed to C M Caldarera.

At least 127 records · Page 7Linked to original sources
PubMed1968

Polyamines and nucleic acid metabolism in chick embryo. Incorporation of labelled precursors into nucleic acids of subcellular fractions and polyribosomal patterns.

1. An increase in polyamine concentration, caused by inhibiting the amine oxidase activities with iproniazid, increased the incorporation of [(3)H]orotic acid into chick-embryo RNA and DNA. On the other hand, a decrease in polyamine concentration, obtained by causing an increase in amine oxidase activities, decreased [(3)H]orotic acid incorporation into nucleic acids. This was particularly evident for nuclear DNA and ribosomal RNA. 2. Polyribosomal patterns obtained by sucrose-density-gradient centrifugation showed highest radioactivity in the regions of 259s and 280s aggregates in those embryos in which the polyamine contents were enhanced, whereas a decrease in the radioactivity was observed when the polyamine concentrations were decreased. 3. The activity of DNA-dependent RNA polymerase, assayed in the same experimental conditions, also varied in the same fashion with changes in polyamine concentration.

Journal Article↗
PubMed1967

Studies on ethionine-induced fatty liver.

1. The effects of the administration of dl-ethionine on some aspects of lipid and nucleotide metabolism in rat liver were studied. 2. In ethionine-treated animals neutral fat was increased, whereas phospholipids and cholesterol were unchanged. Lipogenesis in vitro was inhibited. 3. The concentration of nicotinamide nucleotides, purine nucleotides and pyrimidine nucleotides was decreased. The decrease was due to free adenine nucleotides, inosine nucleotides, uridine nucleotides and cytidine nucleotides. Also, the protein-bound biotin content was lower. 4. In biotin-deficient rats the development of ethionine-induced fatty liver was inhibited. 5. The possibility was considered that ethionine might produce an inhibition of the synthesis of biotin-dependent acetyl-CoA carboxylase.

Acetates↗